猪附红细胞体小鼠感染模型建立条件的优化

doi:10.16431/j.cnki.1671-7236.2017.09.031

摘要/Abstract

摘要：

为探究猪附红细胞体感染特性，进而优化猪附红细胞体小鼠感染模型建立的条件，本试验设计了不同小鼠处理方式感染试验（A试验）、不同病原形式感染试验（B试验）、冻存病原感染试验（C试验）和小鼠模型血液再感染小鼠试验（D试验）。通过对每个试验中各组小鼠血液感染情况、感染首现时间、临床症状和平均感染时间的综合评估，分析不同小鼠处理方式、不同病原形式、冻存病原及小鼠模型血液再感染是否对小鼠模型的建立有影响。通过PCR检测、电镜观察和特异基因片段序列测定，进一步鉴定小鼠模型感染的病原与猪附红细胞体是否一致。结果显示，A试验中，昆明小鼠切除脾脏组感染效果最优；B试验中，猪附红细胞体阳性血液样本组感染效果最优；C试验中，阳性血液未冻存组感染效果优于冻存组；D试验中，猪阳性血液样本组、小鼠模型阳性血液样本组无明显差异。PCR检测、电镜观察和特异基因片段序列测定结果显示，小鼠模型感染的病原与猪附红细胞体一致。结果表明，不同小鼠处理方式、不同病原形式和病原的冻存等条件对猪附红细胞体小鼠感染模型的感染效果均有影响，通过对比，以猪阳性血液感染切除脾脏的昆明小鼠的方式建立小鼠感染模型效果最优。

关键词: 猪附红细胞体; 小鼠; 感染模型

Abstract:

In order to explore the infection characteristics of Mycoplasma suis(M.suis), and optimize the establishment of infection model of M.suis,this research designed the infection test with different mice (A test), different pathogen forms (B test), frozen pathogens (C test), and studied on mice model of blood reinfection (D test). On the basis of a comprehensive assessment of each test in the blood of mice infection, the first infection time, the clinical symptoms and the average duration of infection, to analyze whether the different treatments and different pathogenic mice forms, cryopreservation of pathogen and the mouse model of blood infection had influence on the establishment of mouse model. PCR detection, electron microscope observation and specific gene fragment sequencing were used to determine whether the pathogen of mice infected with the model was consistent with that of M.suis. The results showed that the infection effect of the spleen group was the best in the A test. In the B test, the infection effect of the M.suis positive blood samples group was the best. In the C test, the infection effect of the non-frozen positive blood group was better than that of the cryopreserved group. In the D test, the positive model of pig blood samples and mice model positive blood group had no significant difference. The results of PCR detection, electron microscope observation and specific gene fragment sequencing showed that the pathogen of mouse model was identical with that of M.suis. The results showed that the infection condition of the mice infected with the mouse model was influenced by the different treatment of the mice, the different forms of pathogens and the cryopreservation of the pathogen,and the model of Kunming mice without spleen infected with M.suis positive blood samples was the best.

Key words: Mycoplasma suis; mouse; infection model

中图分类号:

S855

倪婷婷, 伍生军, 梁晚枫, 许应天, 吴金桐, 薛书江. 猪附红细胞体小鼠感染模型建立条件的优化[J]. 《中国畜牧兽医》, 2017, 44(9): 2761-2766.

NI Ting-ting, WU Sheng-jun, LIANG Wan-feng, XU Ying-tian, WU Jin-tong, XUE Shu-jiang. Optimization of Establishment Conditions of Mouse Model of Mycoplasma suis[J]. , 2017, 44(9): 2761-2766.

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