米非司酮对小鼠精子穿透卵丘细胞层的影响

doi:10.16431/j.cnki.1671-7236.2017.05.003

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (5): 1275-1280.doi: 10.16431/j.cnki.1671-7236.2017.05.003

米非司酮对小鼠精子穿透卵丘细胞层的影响

周崇¹, 黄莉², 石德顺¹, 蒋建荣¹

1. 广西大学亚热带生物资源保护利用国家重点实验室, 南宁 530004;
2. 广西壮族自治区兽医研究所, 广西兽医生物技术重点实验室, 南宁 530001

收稿日期:2016-11-22 出版日期:2017-05-20 发布日期:2017-05-18
通讯作者: 周崇, 石德顺 E-mail:czhou720@126.com;ardsshi@gxu.edu.cn
作者简介:周崇(1976-),男,广西南宁人,博士,研究方向:动物生殖生理学;黄莉(1978-),女,广西南宁人,博士,副研究员,研究方向:病毒分子生物学,E-mail:lhuang405@126.com
基金资助:
广西自然科学基金（2014GXNSFCA118008）；广西博士后基金（Y334002001）

Effects of Mifepristone (RU486) on Sperm/Cumulus Penetration in Mice

ZHOU Chong¹, HUANG Li², SHI De-shun¹, JIANG Jian-rong¹

1. State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, Guangxi University, Nanning 530004, China;
2. Guangxi Key Laboratory of Veterinary Biotechnology, Guangxi Veterinary Research Institute, Nanning 530001, China

Received:2016-11-22 Online:2017-05-20 Published:2017-05-18

摘要/Abstract

摘要：

本研究旨在探讨米非司酮（mifepristone，RU486）对小鼠精子穿透卵丘细胞层的影响。分别添加20 μg/mL RU486到精子获能液或穿卵培养液，检测小鼠精子顶体反应发生比率和穿透卵丘细胞层能力。结果显示，添加RU486能够极显著抑制孕酮（P₄，5 μg/mL）诱导的精子顶体反应（P ＜0.01）；并极显著减少穿透卵丘细胞层到达卵子透明带的精子数量及在卵子-卵丘细胞复合体（OCC）中精子发生顶体反应的比率（P＜0.01）；同时添加P₄和RU486情况下，相比单独添加RU486，虽然P₄能够极显著地促进精子穿透卵丘细胞层到达透明带，但对OCC中的精子顶体反应没有明显作用。综上表明，RU486能够抑制P₄诱导的顶体反应并影响小鼠精子穿透卵丘细胞层过程，揭示P₄/孕酮受体（PGR）通路在小鼠精子穿卵过程中具有重要作用。

关键词: 米非司酮; 孕酮; 精子-卵丘互作; 顶体反应; 精子

Abstract:

This study was aimed to investigate the effects of RU486 (mifepristone) on sperm penetration through the cumulus cells layer during fertilization in mice. After 20 μg/mL RU486 was added into the capacitation or the sperm/cumulus penetration medium, respectively, the experiments were conducted to evaluate the ratio of sperm acrosome reaction and ability of sperm/cumulus penetration. The results showed that the addition of RU486 significantly suppressed 5 μg/mL P₄-induced acrosome reaction in the capacitated sperm (P <0.01), and decreased sperm penetrating through the cumulus matrix and reaching the oocyte zona pellucid (ZP) and also remarkably reduced the percentage of acrosome-reacted sperm within the oocyte-cumulus complex (OCC)(P <0.01). Compared with the addition of RU486 alone, P₄ did not reverse the inhibitory effects of RU486 on the acrosome reaction of sperm within the OCC, though it still improved sperm penetration through the cumulus matrix and reaching the ZP (P <0.01). Therefore, RU486 could inhibit P₄-induced acrosome reaction and decrease sperm penetration through the cumulus matrix, which suggested that P₄/progesteron receptor (PGR) pathway might be very important for sperm penetration through the cumulus cell layer.

Key words: RU486; P₄; sperm-cumulus interaction; acrosome reaction; sperm

中图分类号:

S814.4

周崇, 黄莉, 石德顺, 蒋建荣. 米非司酮对小鼠精子穿透卵丘细胞层的影响[J]. 《中国畜牧兽医》, 2017, 44(5): 1275-1280.

ZHOU Chong, HUANG Li, SHI De-shun, JIANG Jian-rong. Effects of Mifepristone (RU486) on Sperm/Cumulus Penetration in Mice[J]. , 2017, 44(5): 1275-1280.

参考文献

[1] Knobil E, Neill J D. The Physiology of Reproduction[M]. 2nd. New York:Raven Press,1994.
[2] Primakoff P, Myles D G. Penetration, adhesion, and fusion in mammalian sperm-egg interaction[J]. Science, 2002, 296(5576):2183-2185.
[3] Ikawa M, Inoue N, Benham A M, et al. Fertilization:A sperm's journey to and interaction with the oocyte[J]. J Clin Invest, 2010, 120(4):984-994.
[4] Oren-Benaroya R, Orvieto R, Gakamsky A, et al. The sperm chemoattractant secreted from human cumulus cells is progesterone[J].Hum Reprod, 2008, 23(10):2339-2345.
[5] Lishko P V, Botchkina I L, Kirichok Y. Progesterone activates the principal Ca²⁺ channel of human sperm[J].Nature, 2011, 471(7338):387-391.
[6] Hong S J, Chiu P C, Lee K F, et al. Cumulus cells and their extracellular matrix affect the quality of the spermatozoa penetrating the cumulus mass[J]. Fertil Steril, 2009, 92(3):971-978.
[7] Fatehi A N, Zeinstra E C, Kooij R V, et al. Effect of cumulus cell removal of in vitro matured bovine oocytes prior to in vitro fertilization on subsequent cleavage rate[J]. Theriogenolngy, 2002, 57(4):1347-1355.
[8] Li R, Norman G R, Armstrong D T, et al. Oocyte-secreted factor(s) determine functional differences between bovine mural granulosa cells and cumulus cells[J]. Biol Reprod, 2000, 63(3):839-845.
[9] Guidobaldi H A, Teves M E, Uñates D R, et al. Progesterone from the cumulus cells is the sperm chemoattractant secreted by the rabbit oocyte cumulus complex[J].PLoS One, 2008, 3(8):e3040.
[10] Aparicio I M, Garcia-Herreros M, O'Shea L C, et al. Expression, regulation, and function of progesterone receptors in bovine cumulus oocyte complexes during in vitromaturation[J]. Biol Reprod, 2011, 84(5):910-921.
[11] Arck P, Hansen P J, Mulac Jericevic B, et al. Progesterone during pregnancy:Endocrine-immune cross talk in mammalian species and the role of stress[J].Am J Reprod Immunol, 2007, 58(3):268-279.
[12] Falsetti C, Baldi E, Krausz C, et al. Decreased responsiveness to progesterone of spermatozoa in oligozoospermic patients[J]. J Androl, 1993, 14(1):17-22.
[13] Shimada M, Yanai Y, Okazaki T, et al. Hyaluronan fragments generated by sperm-secreted hyaluronidase stimulate cytokine/chemokine production via the TLR2 and TLR4 pathway in cumulus cells of ovulated COCs, which may enhance fertilization[J]. Development, 2008, 135(11):2001-2011.
[14] Baulieu E E. Contragestion and other clinical applications of RU486, an antiprogesterone at the receptor[J]. Science, 1989, 245(4924):1351-1357.
[15] Baulieu E E. RU486 as an antiprogesterone steroid:From receptor to contragestion and beyond[J]. JAMA, 1989, 262(13):808-814.
[16] Juneja S C, Dodson M G. In vitro effect of RU486 on sperm-egg interaction in mice[J]. Am J Obstet Gynecol, 1990, 163(Pt1):216-221.
[17] Zhou C, Kang W, Baba T. Functional characterization of double-knockout mouse sperm lacking SPAM1 and ACR or SPAM1 and PRSS21 in fertilization[J]. J Reprod Dev, 2012, 58(3):330-337.
[18] Richards J S. Ovulation:New factors that prepare the oocyte for fertilization[J]. Mol Cell Endocrinol, 2005, 234(1-2):75-79.
[19] Knobil E, Neill J D. The Physiology of Reproduction[M]. New York:Raven Press,1988.
[20] Jin M, Fujiwara E, Kakiuchi Y, et al. Most fertilizing mouse spermatozoa begin their acrosome reaction before contact with the zona pellucida during in vitro fertilization[J]. Proc Natl Acad Sci USA, 2011, 108(12):4892-4896.
[21] Jang S, Yi L S. RU486 suppresses progesterone-induced acrosome reaction in boar spermatozoa[J]. J Biochem Mol Biol, 2002, 35(6):604-608.
[22] Barón L, Fara K, Zapata-Carmona H, et al. Participation of protein kinases and phosphatases in the progesterone-induced acrosome reaction and calcium influx in human spermatozoa[J]. Andrology, 2016, 4(6):1073-1083.
[23] Chen S H, Dharmarajan A M, Wallach E E, et al. RU486 inhibits ovulation, fertilization and early embryonic development in rabbits:In vivo and in vitrostudies[J]. Fertil Steril, 1995, 64(3):627-633.
[24] Yang J, Serres C, Baulieu E E, et al. RU486 inhibits penetration of human spermatozoa into zona-free hamster oocytes[J]. Int J Androl, 1996, 19(1):61-66.

米非司酮对小鼠精子穿透卵丘细胞层的影响

Effects of Mifepristone (RU486) on Sperm/Cumulus Penetration in Mice

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	雷奇, 任杰, 康杰. 黄芩对ZEA暴露雌性小鼠抗氧化能力、性激素水平和ERα mRNA表达的影响[J]. 中国畜牧兽医, 2023, 50(3): 989-997.
[2]	尹伊, 吕艳秋, 陈璇, 曹立朋, 张君正, 金一. 获能处理对猪精子质量及泛素对顶体酶抑制剂水平调控的影响[J]. 中国畜牧兽医, 2023, 50(1): 174-185.
[3]	曾治钦, 娄文琦, 许静漪, 马烨桦, 张永福, 任小丽, 陈少侃, 闫磊, 郭刚, 张震, 王雅春. 泌乳期中国荷斯坦牛转化孕酮浓度的影响因素分析及其表型预测[J]. 中国畜牧兽医, 2023, 50(1): 186-197.
[4]	张雨阳, 曲星霖, 陈璇, 吕艳秋, 曹立朋, 张君正, 金一. 泛素激活酶抑制剂对猪精子获能状态、膜重构及体外受精的影响[J]. 中国畜牧兽医, 2023, 50(1): 210-219.
[5]	杜鑫泽, 马鑫浩, 张殿琦, 杜嘉伟, 马婧, 谢琨成, 何杰, 昝林森. bta-miR-34b/c和bta-miR-449a/b/c靶基因预测及生物信息学分析[J]. 中国畜牧兽医, 2022, 49(7): 2451-2461.
[6]	任秀美奥, 姚旭东, 蒙亚琦, 郭延华, 唐红, 张译元, 赵兴旺, 王立民, 周平. miR-449b对绵羊早期胚胎发育的影响[J]. 中国畜牧兽医, 2022, 49(7): 2661-2668.
[7]	梁家充, 吕春荣, 洪琼花, 吴国权, 权国波. 云南半细毛羊精子冷冻前后转录组表达差异分析[J]. 中国畜牧兽医, 2022, 49(3): 973-981.
[8]	王轶男, 孔令敏, 徐海峰, 张正文, 李春宇, 尹伊, 金一, 吕艳秋. 解冻稀释液中添加精浆对冻融猪精子品质的影响[J]. 中国畜牧兽医, 2022, 49(2): 615-623.
[9]	黄晓刚, 韩贝贝, 李菊, 张守全. miRNA在哺乳动物配子发生中的作用研究进展[J]. 中国畜牧兽医, 2022, 49(2): 624-630.
[10]	李梦杰, 赵伟刚, 郭肖兰, 顾士钢, 赵全民, 徐超. 冷冻稀释液中添加大豆卵磷脂对梅花鹿冻融精子质量的影响[J]. 中国畜牧兽医, 2022, 49(11): 4327-4334.
[11]	刘在霞, 段仕, 孙燕勇, 吕琦, 付绍印, 何小龙, 张文广, 刘永斌. 绵羊睾丸差异基因及蛋白质互作网络关系研究[J]. 中国畜牧兽医, 2022, 49(1): 1-11.
[12]	许娇霞, 张家新. 附睾精子抗氧化保护机制研究进展[J]. 中国畜牧兽医, 2022, 49(1): 91-97.
[13]	陈璇, 吕艳秋, 韩越, 徐一超, 金一. 海藻糖替代部分甘油对延边黄牛冻融精子质量的影响[J]. 中国畜牧兽医, 2022, 49(1): 256-264.
[14]	蓝群, 谢颖瑜, 曹嘉程, 薛丽娥, 李家权, 赵芳露, 林瑞意, 方绍明, 肖天放. 对香豆酸影响猪常温保存精液品质的研究[J]. 中国畜牧兽医, 2021, 48(8): 2928-2936.
[15]	王娜, 张洁, 海超, 李欣, 李光鹏, 赵跃芳. 牛精子冷冻损伤及其改善方法[J]. 中国畜牧兽医, 2021, 48(6): 2101-2112.