《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (4): 1189-1194.doi: 10.16431/j.cnki.1671-7236.2017.04.035

• 预防兽医 • 上一篇    下一篇

免疫磁珠富集法对牛源大肠杆菌O157∶H7分离鉴定的影响

丁浩, 苏战强, 夏利宁, 王雨朦, 王栋, 张瑾瑜, 张桃, 况玲, 姚刚   

  1. 新疆农业大学动物医学学院, 乌鲁木齐 830052
  • 收稿日期:2016-09-19 出版日期:2017-04-20 发布日期:2017-05-03
  • 通讯作者: 姚刚 E-mail:yaogang516@ sina.com
  • 作者简介:丁浩(1991-),男,新疆霍城人,硕士生,研究方向:肉品质微生物安全,E-mail:1484611364@qq.com;苏战强(1974-),男,新疆乌鲁木齐人,博士,副教授,研究方向:肉品质微生物安全,E-mail:szq00009@163.com丁浩和苏战强对本文有同等贡献,并列为第一作者
  • 基金资助:

    国家自然科学基金项目(31560485)

Influence of Immunomagnetic Enrichment Method for Isolation and Identification of E.coli O157∶H7 from Bovine

DING Hao, SU Zhan-qiang, XIA Li-ning, WANG Yu-meng, WANG Dong, ZHANG Jin-yu, ZHANG Tao, KUANG Ling, YAO Gang   

  1. College of Veterinary Medicine, Xinjiang Agricultural University, Urumqi 830052, China
  • Received:2016-09-19 Online:2017-04-20 Published:2017-05-03

摘要:

为证明免疫磁珠吸附技术对牛源大肠杆菌 O157∶H7分离效率的影响,从新疆五家渠市、伊宁县、昌吉市3个牛场的采集18份粪样、162份肛拭子、10份饲料样、17份水样和36份胴体表面棉拭子样本,经EC肉汤增菌后,分别采用免疫磁珠富集后和直接进行SMAC和MUG试验进行选择性培养,然后对菌体rfbE基因和鞭毛fliC基因进行PCR检测,最后对疑似大肠杆菌 O157∶H7菌用生化试验进行符合性检测。结果2种方法分别从243份样品中分离到8株和4株大肠杆菌 O157∶H7,统计学分析该差异不显著。本试验结果表明,在实践中免疫磁珠吸附技术和普通方法相比虽然在统计学上差异不显著,但确实能够增加大肠杆菌O157∶H7的分离数量,这与以前的相关研究结果基本一致。

关键词: 免疫磁珠; 大肠杆菌 O157∶H7; 分离; 鉴定;

Abstract:

In order to prove the effect of immunomagnetic beads adsorption technology on separation efficiency of bovine E.coli O157∶H7 in practice,a total of 18 fecal samples, 162 anal samples,10 fodder samples,17 water samples and 36 samples of carcass surface cotton were collected from three cattle farms in Wujiaqu,Yining and Changji. The samples were taken by EC bead enrichment and direct SMAC and MUG test,respectively. Then,the bacterial rfbE gene and flagellarfliC gene were detected by PCR. Finally,the suspected E.coli O157∶H7 bacteria were detected by biochemical test. 8 and 4 strains of E.coliO157∶H7 were isolated from 243 samples,respectively. There was no statistical difference between the two methods. This experiment showed that the immunomagnetic beads adsorption technique in practice was not statistically significant compared with the conventional method, but it did increase the number of isolated E.coli O157∶H7,which was consistent with previous research results.

Key words: immunomagnetic beads; E.coli O157∶H7; isolation; identification; bovine

中图分类号: