MUC4和MUC13基因在ETEC F18抗性型和易感型梅山断奶仔猪间的差异表达分析

doi:10.16431/j.cnki.1671-7236.2017.04.003

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (4): 959-964.doi: 10.16431/j.cnki.1671-7236.2017.04.003

MUC4和MUC13基因在ETEC F18抗性型和易感型梅山断奶仔猪间的差异表达分析

钦伟云¹, 甘丽娜¹, 魏宗友², 叶兰², 包文斌¹, 吴圣龙¹

1. 扬州大学江苏省动物遗传繁育与分子设计重点实验室, 扬州 225009;
2. 江苏省太仓市畜牧兽医站, 太仓 215400

收稿日期:2016-10-14 出版日期:2017-04-20 发布日期:2017-05-03
通讯作者: 吴圣龙 E-mail:pigbreeding@163.com
作者简介:钦伟云(1992-),男,浙江湖州人,硕士,研究方向:猪抗病育种,E-mail:qinwy24@163.com
基金资助:
江苏省科技支撑计划重点项目（BE2014357）；苏州市科技局攻关项目（SNG201628）

Differential Expression Analysis of MUC4 and MUC13 Genes Between Resistant and Sensitive Weaned Meishan Piglets to ETEC F18

QIN Wei-yun¹, GAN Li-na¹, WEI Zong-you², YE Lan², BAO Wen-bin¹, WU Sheng-long¹

1. Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design of Jiangsu Province, Yangzhou University, Yangzhou 225009, China;
2. Taicang Animal Husbandry and Veterinary Station of Jiangsu Province, Taicang 215400, China

Received:2016-10-14 Online:2017-04-20 Published:2017-05-03

摘要/Abstract

摘要：

MUC4和MUC13基因作为产肠毒素大肠杆菌（enterotoxigenic Escherichia coil，ETEC） F4的重要抗性候选基因，可能在断奶仔猪抗ETEC F18菌株的感染过程中发挥重要作用，因此本试验运用实时荧光定量PCR法分析了MUC4和MUC13基因在梅山猪ETEC F18抗性型和易感型个体11个组织（心脏、肝脏、脾脏、肺脏、肾脏、胃、肌肉、胸腺、淋巴结、十二指肠和空肠）中的表达水平及差异。结果显示，MUC4和MUC13基因在检测的11个组织中均有不同程度的表达。其中，在胸腺和淋巴组织中，MUC4基因在抗性型个体中的表达水平显著高于易感型个体（P<0.05）；在肺脏组织中，MUC13基因在抗性型个体中的表达水平显著高于易感型个体（P<0.05），且MUC13基因在抗性群体肠道中的表达水平较高。由此推测，MUC4基因在免疫组织、MUC13基因在肠道组织的高表达可在一定程度上提高了机体的免疫能力，保护和润滑肠道，从而抵抗ETEC F18菌株对机体的感染。

关键词: 猪; MUC4基因; MUC13基因; 产肠毒素大肠杆菌F18

Abstract:

MUC4 and MUC13 genes as important candidate genes for enterotoxigenic Escherichia coil (ETEC) F4 resistance,may play an important role in the process of against ETEC F18 infection in weaned piglets. In this study,ETEC F18-resistant and -sensitive weaned Meishan piglets were used,and the expression levels of MUC4 and MUC13 genes in 11 tissues (heart,liver,spleen,lung,kidney,stomach,muscle,thymus,lymph nodes,duodenum and jejunum) were determined by quantitative Real-time PCR. The results showed that MUC4 and MUC13 genes were broadly expressed with different expression levels in all the 11 tissues. In the thymus and lymph tissues,the expression of MUC4 gene in resistant piglets was significantly higher than that in sensitive piglets (P<0.05);In the lung tissue,theMUC13 gene expression level in resistant individuals was significantly higher than that in sensitive individuals (P<0.05),and in the intestinal tissues of duodenum and jejunum, the expression level of MUC13 gene was relatively higher in resistant individuals. Thus we speculated that the high expression of MUC4 gene in immune tissues and MUC13 gene in intestinal tissues might improve the immune ability of piglets,protect and lubricate the intestinal tract, and resist ETEC F18 infection.

Key words: pig; MUC4 gene; MUC13 gene; ETEC F18

中图分类号:

Q786

钦伟云, 甘丽娜, 魏宗友, 叶兰, 包文斌, 吴圣龙. MUC4和MUC13基因在ETEC F18抗性型和易感型梅山断奶仔猪间的差异表达分析[J]. 《中国畜牧兽医》, 2017, 44(4): 959-964.

QIN Wei-yun, GAN Li-na, WEI Zong-you, YE Lan, BAO Wen-bin, WU Sheng-long. Differential Expression Analysis of MUC4 and MUC13 Genes Between Resistant and Sensitive Weaned Meishan Piglets to ETEC F18[J]. , 2017, 44(4): 959-964.

参考文献

[1] Moniaux N,Escande F,Porchet N,et al. Structural organization and classification of the human mucingenes[J].Front Biosci,2001,6:1192-1206.
[2] Corfield A,Myerscough N,Gough M,et al. Glycosylation patterns of mucins in colonic disease[J].Biochem Soc Trans,1995,23(4):840-852.
[3] Satoh S,Hinoda Y,Hayashi T,et al. Enhancement of metastatic properties of pancreatic cancer cells by MUC1 gene encoding an anti-adhesion molecule[J].Int J Cancer,2000,88(4):507-518.
[4] 杨国栋,赵乐.MUC13基因在卵巢癌中的表达及其与临床病理因素的关系[J].中国妇幼保健,2015,30(24):4228-4230.
[5] 朱华.肿瘤分子标志物——MUC4研究进展[J].生物医学工程学杂志,2014,31(1):233-236.
[6] Joller D,Jørgensen C B,Bertschinger H,et al. Refined localization of theEscherichia coli F4ab/F4ac receptor locus on pig chromosome 13[J].Anim Genet,2009,40(5):749-752.
[7] 任军,晏学明,艾华水,等.仔猪断奶前腹泻抗病基因育种技术的创建及应用[J].猪业科学,2012,29(1):44-48.
[8] Jacobsen M,Kracht S S,Esteso G,et al. Refined candidate region specified by haplotype sharing for Escherichia coli F4ab/F4ac susceptibility alleles in pigs[J].Anim Genet,2010,41(1):21-25.
[9] Ren J,Tang H,Yan X,et al.A pig-human comparative RH map comprising 20 genes on pig chromosome 13q41 that harbours the ETEC F4ac receptor locus[J].J Anim Breed Genet,2009,126(1):30-36.
[10] Liu Y,Yin X M,Xia R W,et al. Association between the MUC4 g.243A>G polymorphism and immune and production traits in Large White pigs[J].Turkish Journal of Veterinary and Animal Sciences,2015,39(2):141-146.
[11] 阮国荣,何晓芳,黄晶,等.MUC13腹泻抗性纯合杜洛克种群的选育研究[J].江西农业大学学报,2014,36(1):174-180.
[12] Bao W B,Wu S L,Musa H H,et al.Genetic variation at the alpha-1-fucosyltransferase (FUT1) gene in Asian wild boar and Chinese and Western commercial pig breeds[J].J Anim Breed Genet,2008,125(6):427-430.
[13] 吴正常,董文华,刘颖,等.梅山猪E.coli F18菌株攻毒试验及其表型的鉴定分析[J].畜牧兽医学报,2014,45(10):1608-1615.
[14] Oparina N Y,Sadritdinova A F,Snezhkina A V,et al.Increase in NETO2 gene expression is a potential molecular genetic marker in renal and lung cancers[J].Russ J Genet,2012,48(5):506-512.
[15] 黄礼燕.MUC13研究进展[J].临床与病理杂志,2014,34(3):312-316.
[16] Ren J,Yan X,Ai H,et al. Susceptibility towards enterotoxigenic Escherichia coli F4ac diarrhea is governed by the MUC13 gene in pigs[J].PLoS One,2012,7(9):e44573.
[17] 李俊,许钟峯,张海航,等.环江香猪黏附素13基因多态性及其与仔猪腹泻的相关性分析[J].中国畜牧兽医,2016,43(7):1818-1825.
[18] 董文华,黄小国,夏日炜,等.大白猪MUC4基因多态性及其对mRNA表达和细胞因子水平的作用分析[J].中国畜牧杂志,2016,52(3):1-6.
[19] Zhang B,Ren J,Yan X,et al. Investigation of the porcine MUC13 gene:Isolation,expression,polymorphisms and strong association with susceptibility to enterotoxigenic Escherichia coli F4ab/ac[J].Anim Genet,2008,39(3):258-266.
[20] Bertin A M,Duchet-Suchaux M F.Relationship between virulence and adherence of various enterotoxigenic Escherichia coli:Strains to isolated intestinal epithelial cells from Chinese Meishan and European large white pigs[J].Am J Vet Res,1991,52(1):45-49.

MUC4和MUC13基因在ETEC F18抗性型和易感型梅山断奶仔猪间的差异表达分析

Differential Expression Analysis of MUC4 and MUC13 Genes Between Resistant and Sensitive Weaned Meishan Piglets to ETEC F18

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	陈楷文, 赵云娇, 王磊, 张剑搏, 马玉红, 吴国芳. 不同氧浓度下猪睾丸间质细胞转录组分析[J]. 中国畜牧兽医, 2023, 50(6): 2185-2195.
[2]	韦明邦, 边巴琼达, 肖青青, 段梦琪, 强巴央宗, 商鹏. 藏猪CDKN1B基因克隆、生物信息学及表达分析[J]. 中国畜牧兽医, 2023, 50(6): 2196-2206.
[3]	张方玮, 张琪, 雷亮亮, 孙武胜, 张迪, 张云鹏, 张净搏, 王修权, 张晶, 张树敏. 松辽黑猪HBEGF基因多态性及其与繁殖性状的关联分析[J]. 中国畜牧兽医, 2023, 50(6): 2370-2379.
[4]	孙守湖, 陈志飞, 郭智轩, 俞婷, 王修武, 李松倍, 魏文康, 贺东生. 3种猪冠状病毒N基因密码子偏好性及宿主适应机制研究[J]. 中国畜牧兽医, 2023, 50(6): 2403-2413.
[5]	曹琪, 金清, 袁意, 冯逸雪, 孔丹妮, 陈翔鸿, 吴斌, 宋文博, 汤细彪. 规模化猪场常见呼吸道细菌性病原的流行病学调查[J]. 中国畜牧兽医, 2023, 50(6): 2439-2449.
[6]	史秋颖, 段宝敏, 李颖, 牛登云, 耿晨阳, 周佳伟, 王蕊, 冯敬敬. 2020—2021年中国部分地区猪流行性腹泻病毒S1基因遗传变异分析[J]. 中国畜牧兽医, 2023, 50(6): 2468-2478.
[7]	欧云文, 潘琴, 汪洋, 代军飞, 任绍科, 张洋, 张杰. 猪细小病毒6型ORF2基因的截短表达及多克隆抗体制备[J]. 中国畜牧兽医, 2023, 50(6): 2487-2495.
[8]	潘鹏丞, 雷宗全, 李显, 胡湘云, 秦谦涛, 覃兆鲜, 关志惠, 陈宝剑, 谢炳坤. 陆川猪MYL2基因生物信息学分析、真核表达载体构建及组织表达研究[J]. 中国畜牧兽医, 2023, 50(5): 1796-1806.
[9]	欧阳坤, 魏立民, 孙瑞萍, 解娅立, 齐琪, 李想, 刘圈炜, 晁哲, 张永亮, 陈婷. 姜黄素对海南屯昌猪生长性能、饲料养分表观消化率和血清生化指标的影响[J]. 中国畜牧兽医, 2023, 50(5): 1867-1875.
[10]	吴静璇, 孙征, 马雪莲, 张丽阳, 廖秀冬, 罗绪刚, 李素芬, 吕林. 中国畜禽饲料资源中镁含量的调查研究[J]. 中国畜牧兽医, 2023, 50(5): 1888-1898.
[11]	黎明国, 李清春, 陈鑫, 卢世豪, 何凡, 祁梦凡, 张化鹏, 任玉军, 张庆泽, 符彬彬, 徐梦思, 艾子凯, 闫坤, 冯赟, 华再东, 黄涛, 毕延震. TET去甲基化酶对猪卵母细胞发育的影响[J]. 中国畜牧兽医, 2023, 50(5): 1907-1917.
[12]	王泽平, 王飞飞, 张芮铭, 付言峰, 赵为民, 戴超辉, 程金花, 廖超, 李辉. 苏紫猪SLA-1基因多态性及其抗病潜能分析[J]. 中国畜牧兽医, 2023, 50(5): 1918-1927.
[13]	宋承磊, 张哲, 韦怡林, 杨松柏, 豆雅晴, 李晨雷, 刘蓥珂, 李新建, 杨峰, 李秀领, 王克君, 乔瑞敏, 韩雪蕾. 不同品种、季节和月龄对公猪精液品质的影响[J]. 中国畜牧兽医, 2023, 50(5): 1938-1946.
[14]	卓娜, 朱忠武, 李静, 胡世享, 王建昌, 林华, 陈朝林, 韩佃刚, 艾军, 陈培富. 猪源戊型肝炎病毒ORF2蛋白生物信息学分析及真核表达研究[J]. 中国畜牧兽医, 2023, 50(5): 1959-1970.
[15]	康溥, 任照文, 蔡汝健, 吕宗吉, 黄育浩, 王晓虎. 广东及周边省份猪繁殖与呼吸综合征病毒2型谱系8毒株变异规律和传播研究[J]. 中国畜牧兽医, 2023, 50(5): 2001-2011.