产肠毒素大肠杆菌K88诱导仔猪炎症反应的分子机制

doi:10.16431/j.cnki.1671-7236.2017.01.037

《中国畜牧兽医》 ›› 2017, Vol. 44 ›› Issue (1): 262-267.doi: 10.16431/j.cnki.1671-7236.2017.01.037

产肠毒素大肠杆菌K88诱导仔猪炎症反应的分子机制

李海花^1,2, 朱琪^1,2, 王世琼^1,2, 杨春蕾^1,2, 赵向华^1,2, 乔家运^1,2, 王文杰^1,2

1. 天津市畜牧兽医研究所, 天津 300381;
2. 天津市畜禽健康养殖技术工程中心, 天津 300381

收稿日期:2016-06-14 出版日期:2017-01-20 发布日期:2017-01-19
通讯作者: 乔家运 E-mail:qiaojy1979@126.com
作者简介:李海花(1981-),女,河南周口人,博士,助理研究员,研究方向:微生物学与免疫学,E-mail:lihaihuaok@126.com
基金资助:
国家自然科学基金（31402087）

Molecular Mechanism of the Inflammatory Response Induced by Enterotrxigenic E. coli K88 in Piglets

LI Hai-hua^1,2, ZHU Qi^1,2, WANG Shi-qiong^1,2, YANG Chun-lei^1,2, ZHAO Xiang-hua^1,2, QIAO Jia-yun^1,2, WANG Wen-jie^1,2

1. Tianjin Institute of Animal Husbandry and Veterinary Science, Tianjin 300381, China;
2. Tianjin Engineering Research Center for Livestock and Poultry Health Breeding, Tianjin 300381, China

Received:2016-06-14 Online:2017-01-20 Published:2017-01-19

摘要/Abstract

摘要：

为探讨产肠毒素大肠杆菌（ETEC） K88感染仔猪发生炎症反应的分子机制，试验用ETEC K88灌服断奶仔猪，ELISA法检测攻毒后仔猪血清中白细胞介素8（IL-8）含量，实时荧光定量PCR方法检测淋巴结中Toll样受体2（TLR2）、Toll样受体4（TLR4）及其信号通路相关基因（髓样分化因子88（MyD88）、Toll相互作用蛋白（Tollip）、B细胞淋巴瘤因子3（Bcl3））的mRNA相对表达水平。结果发现，仔猪攻毒ETEC K88后6和24 h血清IL-8含量和淋巴结TLR2/4的表达水平均极显著或显著高于对照组（P<0.01；P<0.05），且感染后24 h显著低于感染后6 h（P<0.05）；仔猪感染ETEC K88后24 h淋巴结中MyD88、Tollip和Bcl3的表达水平均极显著高于对照组（P<0.01），但是感染后6 h时与对照组相比均无显著差异（P>0.05）。综上所述，ETEC K88感染仔猪可能是通过TLR2/4-MyD88信号通路产生炎症因子IL-8，促使仔猪出现炎症反应，且该炎症反应可能受Tollip和Bcl3蛋白的调控而被减弱。

关键词: 大肠杆菌K88; 仔猪; 炎症反应; Toll-样受体; 炎症调控

Abstract:

To investigate the molecular mechanism of the inflammatory response in the piglets infected with enterotoxigenic E. coli (ETEC) K88, piglets were infected with ETEC K88,the IL-8 content in serum of piglets were assayed by ELISA,and the mRNA relative expression levels of TLR2/4 and its signal transduction pathway related genes (MyD88,Tollip and Bcl3) in mesenteric lymph nodes were detected by quantitative Real-time PCR. The results showed that compared with control group,the content of IL-8 in serum and the expressions of TLR2/4 in lymph nodes were all extremely significantly or significantly increased at 6 and 24 h after infection (P<0.01;P<0.05),and the IL-8 content and TLR2/4 mRNA expression at 24 h after infection were all significantly lower than those at 6 h after infection (P<0.05).In addition,the expressions of MyD88,Tollip and Bcl3 in lymph nodes were all extremely significantly increased at 24 h after infection compared with control group (P<0.01), but there was no significant difference between experimental group and control group at 6 h after infection (P>0.05). In conclusion,ETEC K88 infected piglets might produce inflammatory cytokines IL-8 through the TLR2/4-MyD88 signaling pathway,which could promote the inflammatory reaction in piglets. This inflammatory response might be regulated by Tollip and Bcl3,which could weak the inflammatory intensity in piglets.

Key words: E. coli K88; piglet; inflammatory response; Toll-like receptors; inflammatory regulation

中图分类号:

S858.28

李海花, 朱琪, 王世琼, 杨春蕾, 赵向华, 乔家运, 王文杰. 产肠毒素大肠杆菌K88诱导仔猪炎症反应的分子机制[J]. 《中国畜牧兽医》, 2017, 44(1): 262-267.

LI Hai-hua, ZHU Qi, WANG Shi-qiong, YANG Chun-lei, ZHAO Xiang-hua, QIAO Jia-yun, WANG Wen-jie. Molecular Mechanism of the Inflammatory Response Induced by Enterotrxigenic E. coli K88 in Piglets[J]. , 2017, 44(1): 262-267.

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产肠毒素大肠杆菌K88诱导仔猪炎症反应的分子机制

Molecular Mechanism of the Inflammatory Response Induced by Enterotrxigenic E. coli K88 in Piglets

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