PGI2对缺少内源性PGs的绵羊早期胚胎体外发育的影响

doi:10.16431/j.cnki.1671-7236.2016.11.031

《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (11): 3019-3023.doi: 10.16431/j.cnki.1671-7236.2016.11.031

PGI₂对缺少内源性PGs的绵羊早期胚胎体外发育的影响

李贺娟, 秦国嵩, 齐晓龙, 盛熙晖, 刘云海, 郭勇, 倪和民

北京农学院, 北京 102206

收稿日期:2016-04-15 出版日期:2016-11-20 发布日期:2016-11-18
通讯作者: 倪和民 E-mail:nihemin@aliyun.com
作者简介:李贺娟(1982-),女,天津人,硕士,助理研究员,研究方向:产科与胚胎工程,E-mail:lihejuan918@163.com
基金资助:
2013年度北京市教委北京市属高等学校创新团队建设与教师职业发展计划项目“体细胞转基因克隆肉牛新品系培育与利用”（PXM2013_014207_000067）

Effect of PGI₂ on Potential Development of in vitro Produced Ovine Embryos Which Lacking with Their Endocrinal PGs

LI He-juan, QIN Guo-song, QI Xiao-long, SHENG Xi-hui, LIU Yun-hai, GUO Yong, NI He-min

Beijing University of Agriculture, Beijing 102206, China

Received:2016-04-15 Online:2016-11-20 Published:2016-11-18

摘要/Abstract

摘要：

试验旨在探讨添加外源Iloprost（PGI₂的稳定类似物）对缺少内源性前列腺素（PGs）的绵羊早期胚胎体外发育的影响。常规体外受精，通过在体外培养液中单独或联合添加前列腺素合成限速酶COX-1和COX-2的特异性抑制剂SC560和NS398，观察COX-1和COX-2对绵羊早期受精胚胎体外发育的影响。添加抑制剂NS398后，再协同添加不同浓度Iloprost，观察PGI₂对绵羊早期体外胚胎发育的具体作用，并对孵化囊胚的细胞数进行分析。结果显示，在卵裂率和囊胚率方面，单独添加NS398与同时添加SC560和NS398组间差异不显著（P>0.05），而与对照组间差异显著（P<0.05）。添加NS398后，再添加不同浓度的外源性Iloprost可代替胚胎内源性PGI₂的作用，基本消除COX-2抑制剂对绵羊早期胚胎体外发育的不利影响。Iloprost的浓度以1×10^-6 mol/L为宜，H33342染色结果差异不显著（P>0.05）。本试验结果表明，胚胎内源性COX-2对绵羊早期胚胎中PGI₂的合成起主要调控作用。外源添加Iloprost可补偿胚胎内源性PGI₂的缺失作用，解除NS398对COX-2的特异性抑制作用，最终促进绵羊早期胚胎的体外发育。

关键词: 前列环素(PGI₂); 绵羊; 早期胚胎; 体外发育

Abstract:

This study was conducted to investigate the effect of PGI₂ on early embryo development of sheep in vitro lacking of endogenous prostaglandins（PGs).The specific inhibitor NS398 and SC560（specifically inhibiting the activity of COX-1 and/or COX-2）were added into the culture medium respectively to study the effect of COX-1 and COX-2 on embryonic development of sheep.Different concentrations of Iioprost were added respectively into the culture medium to investigate the effect of PGI₂ on early embryo development of sheep in vitro.The results showed that there were no significant differences on the cleavage and blastocyst rates between the group only with NS398 and that with both NS398 and SC560（P>0.05).There were significant differences between the group only with NS398 and control group（P<0.05).After adding NS398,different concentrations of exogenous Iloprost could replace the role of embryonic-derived PGI₂,which substantially eliminated the adverse effects of COX-2 inhibitor on early embryo development in vitro.The appropriate concentration of Iloprost was 1×10^-6 mol/L.H33342 staining showed no significant difference（P>0.05).The results demonstrated that embryonic endogenous COX-2 played a major role in regulating synthesis of PGI₂ in early embryo of sheep.The exogenous Iloprost was able to compensate for the loss of endogenous PGI₂ in embryos and remove the inhibition of NS398 on COX-2,ultimately promoting the development of early sheep embryos in vitro.

Key words: PGI₂; ovine; early embryo; in vitro development

中图分类号:

S814.8

李贺娟, 秦国嵩, 齐晓龙, 盛熙晖, 刘云海, 郭勇, 倪和民. PGI₂对缺少内源性PGs的绵羊早期胚胎体外发育的影响[J]. 《中国畜牧兽医》, 2016, 43(11): 3019-3023.

LI He-juan, QIN Guo-song, QI Xiao-long, SHENG Xi-hui, LIU Yun-hai, GUO Yong, NI He-min. Effect of PGI₂ on Potential Development of in vitro Produced Ovine Embryos Which Lacking with Their Endocrinal PGs[J]. , 2016, 43(11): 3019-3023.

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PGI₂对缺少内源性PGs的绵羊早期胚胎体外发育的影响

Effect of PGI₂ on Potential Development of in vitro Produced Ovine Embryos Which Lacking with Their Endocrinal PGs

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