大雁、斑头雁和天鹅源沙门氏菌血清及脉冲场凝胶电泳分型研究

doi:10.16431/j.cnki.1671-7236.2016.11.010

《中国畜牧兽医》 ›› 2016, Vol. 43 ›› Issue (11): 2873-2879.doi: 10.16431/j.cnki.1671-7236.2016.11.010

大雁、斑头雁和天鹅源沙门氏菌血清及脉冲场凝胶电泳分型研究

杨怀珍, 罗薇, 孔雪英, 葛润洲, 牟亚

西南民族大学生命科学与技术学院, 成都 610041

收稿日期:2016-05-13 出版日期:2016-11-20 发布日期:2016-11-18
通讯作者: 罗薇 E-mail:13678006660@163.com
作者简介:杨怀珍(1990-),女,四川通江人,硕士生,研究方向:动物传染病的诊断与防治,E-mail:52583123@qq.com
基金资助:
四川科技厅应用研究丙型副伤寒沙门氏菌感染动物模型的建立及其致病机理的研究（2013JY0045）；西南民族大学研究生创新型科研项目（CX2016SZ078）

PFGE Genotyping and Serotype Analysis of Salmonella Isolated from Goose,Anserindicus and Cygnus

YANG Huai-zhen, LUO Wei, KONG Xue-ying, GE Run-zhou, MOU Ya

College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China

Received:2016-05-13 Online:2016-11-20 Published:2016-11-18

摘要/Abstract

摘要：

为了解大雁、斑头雁及天鹅源沙门氏菌流行特征和脉冲场凝胶电泳（PFGE）分型，本研究对33株沙门氏菌进行血清型鉴定，采用PFGE对其进行基因分型，并用BioNumerics 6.6软件进行聚类分析。鉴定出丙型副伤寒沙门氏菌6株、汤卜逊沙门氏菌19株、乙型副伤寒沙门氏菌1株、伊鲁木沙门氏菌1株、奥斯陆沙门氏菌1株、5株未定型。PFGE聚类分型共分为16个型别，其相似度为53.7%~100.0%，且大多数菌株与人源菌株相似度超过80%。结果表明，大雁、斑头雁及天鹅源沙门氏菌相同血清型PFGE带型高度相似，可能来自同一克隆株，不同血清型PFGE带型差别较大。

关键词: 沙门氏菌; PFGE分型; 血清型

Abstract:

To understand the Salmonella epidemic characteristics and pulsed field gel electrophoresis（PFGE）genotyping of goose,Anserindicus and Cygnus,33 strains of Salmonella were serotype typed by PFGE and genotyped with BioNumerics 6.6 cluster analysis software.The results showed that there were 16 PFGE patterns,which displayed 6 strains Salmonella paratyphi C,19 strains Salmonella thompson,1 strains Salmonella paratyphi B,1 strain Salmonella irumu,1 strain Salmonella oslo,5 undifferentiated.PFGE similarity is about 53.7% to 100.0%,and most of the strains and anthropogenic strains similarity was more than 80%.The results showed that goose,Anserindicus and Cygnus Salmonella serotypes same PFGE type with a high degree of similarity,and possibly from the same clone,quite different serotypes PFGE band pattern difference.

Key words: Salmonella; PFGE genotyping; serotype

中图分类号:

S852.61

杨怀珍, 罗薇, 孔雪英, 葛润洲, 牟亚. 大雁、斑头雁和天鹅源沙门氏菌血清及脉冲场凝胶电泳分型研究[J]. 《中国畜牧兽医》, 2016, 43(11): 2873-2879.

YANG Huai-zhen, LUO Wei, KONG Xue-ying, GE Run-zhou, MOU Ya. PFGE Genotyping and Serotype Analysis of Salmonella Isolated from Goose,Anserindicus and Cygnus[J]. , 2016, 43(11): 2873-2879.

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大雁、斑头雁和天鹅源沙门氏菌血清及脉冲场凝胶电泳分型研究

PFGE Genotyping and Serotype Analysis of Salmonella Isolated from Goose,Anserindicus and Cygnus

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