奶牛隐性子宫内膜炎诊断技术研究进展

doi:10.16431/j.cnki.1671-7236.2016.03.018

摘要/Abstract

摘要： 奶牛隐性子宫内膜炎在以子宫内膜被嗜中性粒细胞(PMN)持续性的广泛浸润为主要特征,没有直观的临床症状,经常导致奶牛屡配不孕。在中国,由子宫内膜炎引起的奶牛不孕的比例达50.62%,且由隐性子宫内膜炎引起的奶牛不孕占其中的90%。由于隐性子宫内膜炎在发病初期不易被观察到,容易错过最佳治疗时间,造成巨大的经济损失,故如何对其做出正确、及时的诊断成了近年来的研究热点。目前,国内外奶牛隐性子宫内膜炎的诊断方法主要有阴道内窥镜法、细胞刷法、活体组织诊断法、超声诊断法及白血球酯酶法等,但目前这些诊断方法没有统一的判断标准。本文对几种不同的奶牛隐性子宫内膜炎的诊断方法进行了综述,为更早、更准确地诊断隐性子宫内膜炎提供依据。

关键词: 奶牛; 隐性子宫内膜炎; 诊断技术

Abstract: In the absence of clinical symptoms,dairy cows with subclinical endometritis are defined as sustainably extensive infiltration with the polymorphonuclear cells (PMN) in endometrial samples,and it often lead to dairy cow with repeated infertility.In China,the infertility proportion of dairy cows is 50.62% caused by endometritis,in which 90% is caused by subclinical endometritis.It is difficult to be observed during the early stage of subclinical endometritis,and the best treatment time is delayed in the most cases causing huge economic losses.So it has become a research hotspot to make correctly and timely diagnosis of this disease.At present,the main diagnosis methods of dairy cows subclinical endometritis are vagina endoscope method,cell brush,biopsy technology,ultrasonic diagnosis technology,leukocyte esterase method and so on.However there is no uniform criterion.Different diagnosis methods of subclinical endometritis were summarized,analyzed and discussed in this paper to provide the references for the subclinical endometritis diagnosis more accurately and earlier,and reduce the losses of dairy farms.

Key words: dairy cow; subclinical endometritis; diagnosis technology

中图分类号:

S823.9⁺1

闫宝琪, 董书伟, 王东升, 那立冬, 杨洪早, 张世栋, 严作廷. 奶牛隐性子宫内膜炎诊断技术研究进展[J]. , 2016, 43(3): 683-688.

YAN Bao-qi, DONG Shu-wei, WANG Dong-sheng, NA Li-dong, YANG Hong-zao, ZHANG Shi-dong, YAN Zuo-ting. Research Progress on Diagnosis Methods of Subclinical Endometritis in Dairy Cows[J]. , 2016, 43(3): 683-688.

参考文献

[1] 王海燕.奶牛子宫内膜炎的诊断与综合防治措施[J].畜牧与饲料科学,2014,35(4):126-127.
[2] 李宏胜,杨峰,王旭荣,等.兰州地区部分奶牛场子宫内膜炎病原菌分离鉴定及抗生素耐药性研究[J].中国畜牧兽医,2014,41(1):222-226.
[3] Sheldon I M,Dobson H.Postpartum uterine health in cattle[J].Animal Reproduction Science,2004,82-83:295-306.
[4] 张世栋,严作廷,王东升,等.奶牛隐性子宫内膜炎的生物学诊疗想法[A].中国奶业协会第26次繁殖学术年会暨国家肉牛牦牛/奶牛产业技术体系第3届全国牛病防治学术研讨会论文集[C].中国:兰州,2011.
[5] Giuliodori M J,Magnasco R P,Becu-Villalobos D,et al.Clinical endometritis in an Argentinean herd of dairy cows:Risk factors and reproductive efficiency[J].Journal of Dairy Science,2013,96(1):210-218.
[6] 李德军.奶牛隐性和临床型子宫内膜炎病理机制研究[D].湖北:华中农业大学,2010.
[7] Melcher Y,Prunner I,Drillich M.Degree of variation and reproducibility of different methods for the diagnosis of subclinical endometritis[J].Theriogenology,2014,82(1):57-63.
[8] 尹红斌,李素华,郭定宗,等.奶牛产后子宫内膜炎分类及研究进展[J].中国奶牛,2009,2:36-39.
[9] Kasimanickam R,Duffield T F,Foster R A,et al.Endometrial cytology and ultrasonography for the detection of subclinical endometritis in postpartum dairy cows[J].Theriogenology,2004,62(1-2):9-23.
[10] 王建发,林玉才,于周滨.子宫刮片法检测奶牛子宫内膜炎的方法介绍[J].中国奶牛,2009,7:36-37.
[11] Foldi J,Kulcsar M,Pecsi A,et al.Bacterial complications of postpartum uterine involution in cattle[J].Animal Reproduction Science,2006,96(3-4):265-281.
[12] Barlund C S,Carruthers T D,Waldner C L,et al.A comparison of diagnostic techniques for postpartum endometritis in dairy cattle[J].Theriogenology,2008,69(6):714-723.
[13] 胡路锋.奶牛产后不同阶段子宫炎监测及其诊断方法的研究[D].石河子:石河子大学,2014.
[14] Westermann S,Drillich M,Kaufmann T B,et al.A clinical approach to determine false positive findings of clinical endometritis by vaginoscopy by the use of uterine bacteriology and cytology in dairy cows[J].Theriogenology,2010,74(7):1248-1255.
[15] Prunner I,Wagener K,Pothmann H,et al.Risk factors for uterine diseases on small-and medium-sized dairy farms determined by clinical,bacteriological,and cytological examination[J].Theriogenology,2014,82(6):857-865.
[16] Madoz L V,Giuliodori M J,Jaureguiberry M,et al.The relationship between endometrial cytology during estrous cycle and cutoff points for the diagnosis of subclinical endometritis in grazing dairy cows[J].Journal of Dairy Science,2013,96(7):4333-4339.
[17] Machado V S,Knauer M L,Bicalho G.A novel diagnostic technique to determine uterine health of Holstein cows at 35 days postpartum[J].Journal of Dairy Science,2012,95(3):1349-1357.
[18] Barański W,Podhalicz-Dzięgielewska M,Zduńczyk S,et al.The diagnosis and prevalence of subclinical endometritis in cows evaluated by different cytologic thresholds[J].Theriogenology,2012,78(9):1939-1947.
[19] Gilbert R O,Shin S T,Guard C L,et al.Prevalence of endometritis and its effects on reproductive performance of dairy cows[J].Theriogenology,2005,64(9):1879-1888.
[20] Galvao K N,Frajblat M,Brittin S B,et al.Effect of prostaglandin F2alpha on subclinical endometritis and fertility in dairy cows[J].Journal of Dairy Science,2009,92(10):4906-4913.
[21] Deguillaume L,Geffré A,Desquilbet L,et al.Effect of endocervical inflammation on days to conception in dairy cows[J].Journal of Dairy Science,2012,95(10):1776-1783.
[22] Cengiz M,Kanca H,Salar S,et al.Endometrial echotexture parameters in Turkish Saanen goats (Akkeci) during oestrus and early pregnancy[J].Animal Reproduction Science,2014,146(1-2):27-33.
[23] Morel O,Pachy F,Chavatte-Palmer P,et al.Correlation between uteroplacental three-dimensional power Doppler indices and true uterine blood flow:Evaluation in a pregnant sheep model[J].Ultrasound Obstet Gynecol,2010,36(5):635-640.
[24] Polat B,Cengiz M,Cannazik O,et al.Endometrial echotexture variables in postpartum cows with subclinical endometritis[J].Animal Reproduction Science,2015,155:50-55.
[25] Meira E J,Henriques L C,Sa L R,et al.Comparison of ultrasonography and histopathology for the diagnosis of endometritis in Holstein-Friesian cows[J].Journal of Dairy Science,2012,95(12):6969-6973.
[26] Chapwanya A,Meade K G,Doherty M L,et al.Histopathological and molecular evaluation of Holstein-Friesian cows postpartum:Toward an improved understanding of uterine innateimmunity[J].Theriogenology,2009,71(9):1396-1407.
[27] Sheldon I M,Lewis G S,Leblanc S,et al.Defining postpartum uterine disease in cattle[J].Theriogenology,2006,65(8):1516-1530.
[28] Dobson H,Smith R F,Royal M D.The high-producing dairy cow and its reproductive performance[J].Reproduction in Domestic Animals,2007,42(S2):17-23.
[29] Couto G B,Vaillancourt D H,Lefebvre R C.Comparison of a leukocyte esterase test with endometrial cytology for diagnosis of subclinical endometritis in postpartum dairy cows[J].Theriogenology, 2013,79(1):103-107.
[30] Santos N R,Roman H B,Gilbert R O.The use of leukocyte esterareagent strips for diagnosis of subclinical endometritis in dairy cows[J].Theriogenology,2006,66:666-672.
[31] Loyi T,Kumar H,Nandi S,et al.Differential expression of pro-inflammatory cytokines in endometrial tissue of buffaloes with clinical and sub-clinical endometritis[J].Research in Veterinary Science,2013,94(2):336-340.
[32] Ghasemi F,Gonzalez-Cano P,Griebel P J,et al.Proinflammatory cytokine gene expression in endometrial cytobrush samples harvested from cows with and without subclinical endometritis[J].Theriogenology,2012,78(7):1538-1547.
[33] 张辉,陶大勇,严光文,等.隐性子宫内膜炎患牛子宫黏液和血清中离子变化的研究[J].黑龙江畜牧兽医,2012,21(48):103-104.
[34] Martinez N,Sinedino L D P,Bisinotto R S,et al.Effect of induced subclinical hypocalcemia on physiological responses and neutrophil function in dairy cows[J].Journal of Dairy Science,2014,97(2):874-887.