《中国畜牧兽医》---唯一指定的官方网站 ›› 2015, Vol. 42 ›› Issue (9): 2399-2404.doi: 10.16431/j.cnki.1671-7236.2015.09.028

• 生理生化 • 上一篇    下一篇

氢化物发生—原子荧光光谱法测定牛肉中的硒含量

刘丽娜, 徐发婷, 赵方红, 秦顺义, 姚婉, 刘佳, 李玉竹   

  1. 天津农学院动物科学与动物医学学院, 天津 300384
  • 修回日期:2015-05-04 出版日期:2015-09-20 发布日期:2015-09-25
  • 通讯作者: 秦顺义 E-mail:qinshunyi@163.com
  • 作者简介:刘丽娜(1989-),女,河南郑州人,硕士生,研究方向:动物营养与免疫,E-mail:15022289820@163.com
  • 基金资助:

    国家自然科学基金(31101867);国家级大学生创新创业训练计划项目(201410061011)

Detection of Selenium Content in Beef by Hydride Atomic Fluorescence Spectrometry

LIU Li-na, XU Fa-ting, ZHAO Fang-hong, QIN Shun-yi, YAO Wan, LIU Jia, LI Yu-zhu   

  1. College of Animal Science and Veterinary Medicine, Tianjin Agricultural University, Tianjin 300384, China
  • Revised:2015-05-04 Online:2015-09-20 Published:2015-09-25

摘要: 本试验采用了氢化物发生—原子荧光光谱法测定牛肉中的硒,通过消解液、预还原剂、介质酸的选择等优化了试验条件并测定其检出限、精密度、加标回收率等。结果显示本试验选用2:1的混合酸作为消解液,6 mol/L HCl作为预还原剂,体积比为10%的HCl作为介质酸。硒在0~8 μg/L的范围内呈良好的线性关系,相关系数为0.9999,检出限为0.01 ng/mL。在20、30和40 μg/L 3个添加水平下牛肉的回收率为94%~104%,相对标准偏差(RSD)为3.80%。该方法具有简便、有效等特点,对肉制品中硒的测定具有一定的参考意义。

关键词: 硒; 牛肉; 氢化物发生— 原子荧光光谱法

Abstract: In this assay, we detected the selenium content in beef by hydride atomic fluorescence spectrometry.The conditions of the test were optimized by the selections of clearing up solution, pre-reducing agent, medium acid and so on, the limits of detection, precision and the standard addition recovery were determined.The results showed that 2:1 mixed acid as the clearing up solution, 6 mol/L hydrochloric acid as pre-reducing agent and the volume ratio of 10% hydrochloric acid as acid medium were chosen, the calibration curve was linear in the range from 0 to 8.0 μg/L with correlation coefficient was 0.9999.The limit of detection (LOD) was 0.01 ng/mL.At three adding levels of 20, 30 and 40 μg/L for selenium, the recovery of beef was 94% to 104% with RSD of 3.80%.This method had the characteristics of simple and effective, it had important referential significance for the detection of selenium in meat.

Key words: selenium; beef; hydride atomic fluorescence spectrometry

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