黄芪灵芝口服液对免疫新城疫疫苗雏鸡免疫功能的影响

doi:10.16431/j.cnki.1671-7236.2015.07.034

›› 2015, Vol. 42 ›› Issue (7): 1859-1864.doi: 10.16431/j.cnki.1671-7236.2015.07.034

黄芪灵芝口服液对免疫新城疫疫苗雏鸡免疫功能的影响

周泽伟¹, 李楠¹, 余峥嵘¹, 郑玲敏¹, 鲁倩倩¹, 张雨梅^1,2

1. 扬州大学兽医学院, 扬州 225009;
2. 江苏省动物重要疫病与人兽共患病防控协同创新中心, 扬州 225009

收稿日期:2014-12-23 出版日期:2015-07-20 发布日期:2015-07-23
通讯作者: 张雨梅 E-mail:zym@yzu.edu.cn
作者简介:周泽伟(1988-),男,河南焦作人,硕士生,研究方向:兽医药理与毒理学,E-mail:dzwzhilu@163.com;李楠(1991-),男,江苏盐城人,硕士生,研究方向:兽医药理与毒理学,E-mail:757402133@qq.com
基金资助:
江苏高校优势学科建设工程项目;江苏省研究生实践创新计划(SJLX_0618)

Effects of the Oral Liquid of Astragalus and Lucidum Polysaccharides on Immunological Function in Chickens Immuned with ND Vaccine

ZHOU Ze-wei¹, LI Nan¹, YU Zheng-rong¹, ZHENG Ling-min¹, LU Qian-qian¹, ZHANG Yu-mei^1,2

1. College of Veterinary Medicine, Yangzhou Univercity, Yangzhou 225009, China;
2. Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, China

Received:2014-12-23 Online:2015-07-20 Published:2015-07-23

摘要/Abstract

摘要： 为研究黄芪灵芝口服液对雏鸡免疫功能的影响,本试验将180只14日龄雏鸡随机分成9组并通过点眼滴鼻免疫新城疫(ND)疫苗。1~3组分别是黄芪灵芝口服液高、中和低剂量组;4~6组分别是环磷酰胺+黄芪灵芝口服液高、中和低剂量组;7~9组分别为环磷酰胺组、阳性对照组及生理盐水对照组。分别于免疫后的第7、14、21、28天测定脾脏淋巴细胞增殖和抗体滴度的动态变化。结果表明,黄芪灵芝口服液能提高雏鸡脾脏淋巴细胞的增殖能力和促进新城疫病毒(NDV)抗体的产生,能一定程度上缓解环磷酰胺的免疫抑制,其中黄芪灵芝口服液低剂量组对脾脏淋巴细胞增殖效果最好,黄芪灵芝口服液可作为一种新型的免疫增强剂。

关键词: 黄芪灵芝; 雏鸡; 脾脏淋巴细胞增殖; 抗体滴度; 免疫增强剂

Abstract: In order to study the effects of the oral liquid of Astragalus and Lucidum polysaccharides on immunological function in chickens,one hundred and eighty 14-day-old chickens were randomly divided into 9 groups and inoculated with the Newcastle disease vaccine.1 to 3 experimental groups were respectively the oral liquid of Astragalus and Lucidum polysaccharides high,middle and low dose groups;4 to 6 experimental groups were respectively the oral liquid of Astragalus and Lucidum polysaccharides with cyclophosphamide high,middle and low dose groups;7 to 9 experimental groups were respectively cyclophosphamide group,positive control group and saline control group.The proliferation index of spleen lymphocyte and Newcastle disease virus antibody level by HI method were determined on days 7,14,21 and 28 after vaccination.The results showed that the oral liquid of Astragalus and Lucidum polysaccharides could apparently promote lymphocyte proliferation and enhance antibody titer,and alleviate the immunosupperession of the chickens in some degree,the low dose of the oral liquid of Astragalus and Lucidum polysaccharides promoted lymphocyte proliferation best,implying that the oral liquid of Astragalus and Lucidum polysaccharides would be expected as a candidate of new-type immunopotentiator.

Key words: Astragalus and Lucidum; chicken; proliferation of spleen lymphocyte; antibody titer; immunopotentiator

中图分类号:

S852.4

周泽伟, 李楠, 余峥嵘, 郑玲敏, 鲁倩倩, 张雨梅. 黄芪灵芝口服液对免疫新城疫疫苗雏鸡免疫功能的影响[J]. , 2015, 42(7): 1859-1864.

ZHOU Ze-wei, LI Nan, YU Zheng-rong, ZHENG Ling-min, LU Qian-qian, ZHANG Yu-mei. Effects of the Oral Liquid of Astragalus and Lucidum Polysaccharides on Immunological Function in Chickens Immuned with ND Vaccine[J]. , 2015, 42(7): 1859-1864.

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黄芪灵芝口服液对免疫新城疫疫苗雏鸡免疫功能的影响

Effects of the Oral Liquid of Astragalus and Lucidum Polysaccharides on Immunological Function in Chickens Immuned with ND Vaccine

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