从江香猪基因组甲基化修饰与体重之间的相关性研究

doi:10.16431/j.cnki.1671-7236.2015.04.030

›› 2015, Vol. 42 ›› Issue (4): 973-979.doi: 10.16431/j.cnki.1671-7236.2015.04.030

从江香猪基因组甲基化修饰与体重之间的相关性研究

林如涛^1,2, 冉雪琴², 王嘉福^1,3

1. 贵州大学省农业生物工程研究院, 贵阳 550025;
2. 贵州大学动物科学学院, 贵阳 550025;
3. 贵州大学生命科学学院, 贵阳 550025

收稿日期:2014-12-18 出版日期:2015-04-20 发布日期:2015-05-05
通讯作者: 王嘉福 E-mail:jfwang@gzu.edu.cn
作者简介:林如涛(1990-),男,重庆人,硕士生,研究方向:动物生理与分子生物学,E-mail:233715956@qq.com
基金资助:
国家高技术研究发展计划(863计划)课题(2013AA102503);贵州省农业科技攻关项目(黔科合NY[2013]3073号);贵州省科技创新人才团队建设专项;贵州省动物遗传育种与生物技术科技创新人才团队(黔科合人才团队2009-4006);贵州大学“本科教学工程”项目(BKJX2012007)

Relationship between Genomic DNA Methylation and Body Weight of Congjiang Xiang Pig

LIN Ru-tao^1,2, RAN Xue-qin², WANG Jia-fu^1,3

1. Institute of Agricultural Bioengineering, Guizhou University, Guiyang 550025, China;
2. College of Animal Science, Guizhou University, Guiyang 550025, China;
3. College of Life Sciences, Guizhou University, Guiyang 550025, China

Received:2014-12-18 Online:2015-04-20 Published:2015-05-05

摘要/Abstract

摘要： 为了探索从江香猪生长慢的表观遗传学机理,本试验应用甲基化敏感扩增多态性(methylation-sensitive amplifying polymorphism,MSAP)技术,将60头20 d从江香猪分为高体重组和低体重组,研究血液和肝脏基因组DNA中胞嘧啶的甲基化程度,并测定差异的甲基化片段核苷酸序列.结果发现,MSAP分析得到5 977条带;与低体重组相比,高体重组的血液和肝脏总甲基化水平较低,两组的血液半甲基化水平均较肝脏中的高,全/总甲基化水平较低.提示香猪血液和肝脏基因组的总甲基化程度随体重的增加而下降.经克隆测序得到5条有差异的甲基化条带A1-A5,其中A1和A2源自血液,A3-A5来自肝脏;A1和A4片段为高体重组特有,其余3条片段为低体重组特有;A3片段未找到相关基因,其他4个片段处于代谢或免疫相关的基因内部或5'侧翼区.提示从江香猪基因组的甲基化水平存在个体差异,并与20 d体重相关;得到的4条甲基化片段,将有助于香猪甲基化调控机制的研究.

关键词: 从江香猪; 基因组甲基化; 差异片段; 体重

Abstract: To explore the epigenetics mechanism for the slow growth in Xiang pig, 60 piglets in 20 days were divided into two groups with high or low body weight. The methylation modification at cytosine in the genomic DNA from blood and liver was investigated by methylation-sensitive amplifying polymorphism (MSAP) technique. A total of 5 977 sites were counted based on MSAP results. Compared to the low-weight group, the total DNA methylation level in high-weight group was lower. Although the half DNA methylation levels in blood samples were higher than that in liver samples, the both/total DNA methylation levels in blood were lower than that in liver. It showed that the total DNA methylation level tended to decrease as the increasing of body weight. Five specific fragments from A1 to A5 were sequenced which were differently methylated between two groups. Both of A1 and A4 detected from high-weight group while the others got from low-weight group. Fragment A1 and A2 detected from blood genomic DNA and A3 to A5 from liver samples. A3 was apart far from two genes without coding region for RNA. The others were located in genes related with metabolism and immune. The results indicated that genomic DNA methylation level was different among individual piglet and related with the body weight of Xiang piglet in 20 days. The four specific methylation fragments would be useful for research on the mechanism of growth in Xiang pig breed.

Key words: Congjiang Xiang pig; genomic methylation; specific fragments; body weight

中图分类号:

S813.3

林如涛, 冉雪琴, 王嘉福. 从江香猪基因组甲基化修饰与体重之间的相关性研究[J]. , 2015, 42(4): 973-979.

LIN Ru-tao, RAN Xue-qin, WANG Jia-fu. Relationship between Genomic DNA Methylation and Body Weight of Congjiang Xiang Pig[J]. , 2015, 42(4): 973-979.

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从江香猪基因组甲基化修饰与体重之间的相关性研究

Relationship between Genomic DNA Methylation and Body Weight of Congjiang Xiang Pig

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