关键词: 犬; 红细胞; 低温保存; 渗透脆性; 离子浓度

Abstract: MAP solution with different addition proportions were selected to carry out low-temperature preservation of canine erythrocyte for screening suitable adding proportion. Whole blood was collected from a healthy cyan back male canine by foreleg the inside of the subcutaneous cephalic vein, which centrifuged for obtaining erythrocyte. Accordingly the different proportions of erythrocyte to MAP solution (2∶1,2.5∶1 and 3∶1) were used for the preservation of canine erythrocyte at 4 ℃, and each group was preserved for 30 days, the number of erythrocyte and K⁺, Na⁺, Cl^- concentrations were measured at 0, 10,20 and 30 d, respectively. The result showed that the number of erythrocyte reduction, K⁺ concentration augmenter and Na⁺, Cl^-concentration decrements were significantly lower in 2.5∶1 group than 2∶1 and 3∶1 groups （P<0.05）. A better effect was obtained when the proportion of erythrocyte to MAP solution was 2.5∶1, therefore, 2.5∶1 was the suitable proportion for canine erythrocyte low-temperature preservation.

Key words: canine; erythrocyte; low-temperature preservation; osmotic fragility; ionic concentration