牦牛产志贺毒素大肠杆菌主要黏附因子相关基因的分子流行病学调查

›› 2013, Vol. 40 ›› Issue (1): 153-157.

牦牛产志贺毒素大肠杆菌主要黏附因子相关基因的分子流行病学调查

宋定州¹, 于学辉¹, 汤承¹, 李键^1,2, 冉丹丹¹

1. 西南民族大学生命科学与技术学院, 四川成都 610041;
2. 西南民族大学青藏高原生态保护与畜牧业高科技研发基地, 四川成都 610041

收稿日期:2012-05-17 出版日期:2013-01-20 发布日期:2013-01-14
通讯作者: 于学辉(1961-),男,四川人,博士,副教授,主要从事动物传染病防治研究工作。E-mail:yuxuehui@yahoo.cn;Tel:028-85522867 E-mail:yuxuehui@yahoo.cn
作者简介:宋定州(1985-),男,湖北人,硕士生,主要从事动物传染病防治研究工作。
基金资助:
中央高校基本科研业务费科研平台建设项目(11NPT03);国家公益性行业(农业)科研专项经费(201203009)。

Molecular Epidemiological Investigation of Genes Related with the Major Adhesion Factors in Shiga Toxin-producing Escherichia coli from Yak

SONG Ding-zhou¹, YU Xue-hui¹, TANG Cheng¹, LI Jian^1,2, RAN Dan-dan¹

1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China;
2. The High-tech Research and Development Base for Ecological Protection and Animal Husbandry in Qinghai-Tibet Plateau, Southwest University for Nationalities, Chengdu 610041, China

Received:2012-05-17 Online:2013-01-20 Published:2013-01-14

摘要/Abstract

摘要： 为了解中国牦牛产志贺毒素的大肠杆菌(Shiga toxin-producing Escherichia coli, STEC)中主要黏附因子的流行情况,采用PCR方法对来自四川甘孜阿坝等地区健康牦牛的70株STEC的eae、saa、iha 3种与黏附相关的毒力基因进行检测,并对部分含有相关黏附因子的阳性分离株的毒力基因进行了克隆及序列分析。结果显示,牦牛STEC中saa、iha的阳性率分别为71.42%(50/70)和78.57%(55/70),无eae基因序列(0/70),saa、iha的测序结果与GenBank上序列的同源性分别为100%和93%~99%。健康牦牛分离的STEC无LEE毒力岛编码eae,其他的一些与黏附相关的主要毒力基因saa、iha的携带率较高。

关键词: 牦牛; 产志贺毒素的大肠杆菌; 黏附基因

Abstract: The assay was aimed to investigate the prevalence of the major adhension factors in Shiga toxin-producing Escherichia coli from Yak.By using the method of PCR to detect the adhension factors of eae,saa,iha genes in the 70 STEC isolates from the healthy Yak in Sichuan Ganzi,Aba and other places,some of the adhension genes were cloned and sequenced. The results showed that the carried rates of saa and iha genes were 71.42%(50/70) and 78.57%(55/70) respectively in 70 STEC strains from Yak, none of the STEC isolates carried the eae gene(0/70), the homology of saa and iha genes was 100% and 93% to 99% respectively with the reference sequences in GenBank.STEC isolates from the healthy Yak didn’t have eae gene which encoded by LEE virulence island, the carried rates of other adhension virulence genes saa, iha was higher.

Key words: Yak; Shiga toxin-producing Escherichia coli; adhension gene

中图分类号:

S852.61

宋定州, 于学辉, 汤承, 李键, 冉丹丹. 牦牛产志贺毒素大肠杆菌主要黏附因子相关基因的分子流行病学调查[J]. , 2013, 40(1): 153-157.

SONG Ding-zhou, YU Xue-hui, TANG Cheng, LI Jian, RAN Dan-dan. Molecular Epidemiological Investigation of Genes Related with the Major Adhesion Factors in Shiga Toxin-producing Escherichia coli from Yak[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(1): 153-157.

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