牛α-乳清蛋白基因5'调控区多态性研究

›› 2013, Vol. 40 ›› Issue (1): 136-140.

牛α-乳清蛋白基因5'调控区多态性研究

杨永强¹, 龚俞², 焦仁刚², 惠嫣婷¹, 刘若余¹

1. 贵州大学动物科学学院, 高原山地动物遗传育种与繁殖教育部重点实验室, 贵州省动物遗传育种与繁殖重点实验室, 贵州贵阳 550025;
2. 贵州省畜牧技术推广站, 贵州贵阳 550001

收稿日期:2012-07-25 出版日期:2013-01-20 发布日期:2013-01-14
通讯作者: 刘若余(1963-),男,湖南人,教授,硕士生导师,博士,研究方向:分子遗传与动物育种。E-mail:liury04@163.com E-mail:liury04@163.com
作者简介:杨永强(1989-),男,四川人,硕士生,研究方向:分子遗传与动物育种。
基金资助:
贵州省重大科技专项计划项目(黔科合重大专项字[2011]6009号)。

Study on the Polymorphisms of LALBA Gene 5' Regulation Region in Cattle

YANG Yong-qiang¹, GONG Yu², JIAO Ren-gang², HUI Yan-ting¹, LIU Ruo-yu¹

1. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou Key Laboratory of Animal Genetics, Breeding and Reproduction, College of Animal Sciences, Guizhou University, Guiyang 550025, China;
2. Animal Science and Technology Station of Guizhou, Guiyang 550001, China

Received:2012-07-25 Online:2013-01-20 Published:2013-01-14

摘要/Abstract

摘要： 试验选择品种差异较大的贵州荷斯坦奶牛和务川黑牛构建不同DNA池,设计1对引物分别扩增2个牛种α-乳清蛋白(alpha-lactalbumin, LALBA)基因5'调控区及第1外显子部分序列总长1126 bp。结果表明,LALBA基因5'调控区存在5个SNPs位点:T-114C、C-166T、A-225C、C-344T、T-778C,T-114C仅在务川黑牛表现多态性。生物信息学软件预测LALBA基因核心启动子区及转录因子结合位点,SNP位点导致11个转录因子结合位点消失,其中1个位于核心启动子区,产生5个新的转录因子结合位点。突变前后RNA二级结构发生明显改变,目标序列未发现CpG岛。

关键词: 贵州荷斯坦奶牛; 务川黑牛; LALBA基因; SNP; 启动子

Abstract: In order to analyze the polymorphisms of LALBA gene 5'regulation region in cattle. Two cattle breeds (Wuchuan Black cattle and Guizhou Holstein cow) with significant difference in breeds property were selected to construct DNA pools, a total of 1126 bp sequences of LALBA 5'regulation region and part of exon 1 were obtained by designing 1 pair specific primers. The results showed that 5 single nucleotide polymorphisms (SNPs) were found in the 5'regulation region which included T-114C, C-166T, A-225C, C-344T, T-778C respectively and T-114C was specific to Wuchuan Black cattle. Furthermore, bioinformatics tools were used to predict the core region of the promoter and identify various transcription factors binding sites. It demonstrated that 5 new transcription factors binding sites emerged while 11 previous transcription factors binding sites disappeared which included one located in the core region of promoter results from the SNPs found in this study, the remarkable change of secondary structure of RNA was also detected but the CpG islands was not found by using various softwares.

Key words: Guizhou Holstein cow; Wuchuan Black cattle; LALBA gene; SNP; promoter

中图分类号:

杨永强, 龚俞, 焦仁刚, 惠嫣婷, 刘若余. 牛α-乳清蛋白基因5'调控区多态性研究[J]. , 2013, 40(1): 136-140.

YANG Yong-qiang, GONG Yu, JIAO Ren-gang, HUI Yan-ting, LIU Ruo-yu. Study on the Polymorphisms of LALBA Gene 5' Regulation Region in Cattle[J]. , 2013, 40(1): 136-140.

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