猪嵴病毒RT-PCR检测方法的建立及应用

›› 2013, Vol. 40 ›› Issue (1): 42-46.

猪嵴病毒RT-PCR检测方法的建立及应用

张文波¹, 冷闯¹, 段俊², 邓舜洲¹

1. 江西农业大学动物科学技术学院, 江西南昌 330045;
2. 南昌金牧动物保健服务有限公司, 江西南昌 330013

收稿日期:2012-06-26 出版日期:2013-01-20 发布日期:2013-01-14
通讯作者: 邓舜洲(1968-),男,江西人,博士,主要从事动物传染病研究。E-mail:shzhdeng@163.com E-mail:shzhdeng@163.com
作者简介:张文波(1972-),男,河南人,博士,主要从事动物传染病病原研究。
基金资助:
国家自然科学基金项目(31160498/C1803)。

Establishment and Application of RT-PCR Detection of Pocrine Kobuvirus

ZHANG Wen-bo¹, LENG Chuang¹, DUAN Jun², DENG Shun-zhou¹

1. College of Animal Science and Technology, Jiangxi Agricultural University, Nanchang 330045, China;
2. Nanchang Jinmu Animal Health Services Co., Ltd., Nanchang 330013, China

Received:2012-06-26 Online:2013-01-20 Published:2013-01-14

摘要/Abstract

摘要： 根据GenBank中登录的猪嵴病毒3D基因保守序列,设计合成1对引物,以腹泻哺乳仔猪的小肠为起始材料,建立了猪峭病毒的RT-PCR检测方法,并用该方法对收集的50份哺乳仔猪腹泻样本进行检测。结果显示,该方法对模板的最小检测量是2.3 pg,检测猪其他常见致腹泻病毒均为阴性,同一样品3次试验结果一致;所扩增的序列经测序分析均为猪嵴病毒的3D核苷酸序列;仔猪腹泻样本的猪嵴病毒检出率达58%。结果表明,该方法敏感度高、重复性和特异性良好,可用于猪嵴病毒的分子流行病学调查和临床病例的快速诊断。

关键词: 猪嵴病毒; RT-PCR; 同源性分析

Abstract: A detection method for porcine kobuvirus (PKV) with RT-PCR technique was established to detect the 3D gene of PKV from the intestinal tract of diarrhea piglets, with the detection limit about 2.3 pg RNA. 50 samples of diarrhea piglets were detected by the RT-PCR method, the PKV positive rate of samples was 58 percent. The PCR detection method established in this study has the characteristics of sensitivity, high specificity and good repeatability, and could be used in molecular epidemiological investigation and rapid clinical diagnosis of PKV.

Key words: porcine kobuvirus; RT-PCR; homology analysis

中图分类号:

S852.65

张文波, 冷闯, 段俊, 邓舜洲. 猪嵴病毒RT-PCR检测方法的建立及应用[J]. , 2013, 40(1): 42-46.

ZHANG Wen-bo, LENG Chuang, DUAN Jun, DENG Shun-zhou. Establishment and Application of RT-PCR Detection of Pocrine Kobuvirus[J]. , 2013, 40(1): 42-46.

参考文献

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