茶树菇多糖对小鼠脾脏淋巴细胞免疫调节的影响

doi:10.16431/j.cnki.1671-7236.2023.05.010

摘要/Abstract

摘要： 【目的】试验旨在揭示茶树菇多糖(Agrocybe aegerita polysaccharid,AAP)对小鼠脾脏淋巴细胞免疫调节的影响,为阐明AAP的作用机制及其临床应用提供依据。【方法】以小鼠脾脏淋巴细胞为研究对象,用噻唑蓝(MTT)比色法测定AAP对淋巴细胞的安全浓度,以安全浓度进行后续试验;用MTT法检测不同浓度AAP对小鼠脾脏淋巴细胞增殖的影响,同时设置细胞、脂多糖(LPS)和植物血凝素(PHA)3个对照组,筛选出对小鼠脾脏淋巴细胞增殖能力较强的浓度,用流式细胞仪检测其对T淋巴细胞亚型CD4⁺/CD3⁺和CD8⁺/CD3⁺比值的影响,利用ELISA法检测其对淋巴细胞上清液中白细胞介素-2(IL-2)、IL-4、肿瘤坏死因子-α(TNF-α)和γ-干扰素(IFN-γ)含量的影响。【结果】低于1 000 μg/mL AAP对小鼠脾脏淋巴细胞不产生毒性作用。与对照组相比,不同浓度AAP均可极显著协同LPS和PHA刺激淋巴细胞增殖(P＜0.01);125和250 μg/mL AAP可极显著提高CD4⁺/CD3⁺和CD8⁺/CD3⁺比值(P＜0.01),显著或极显著升高淋巴细胞分泌的细胞因子IL-2、IL-4、TNF-α和IFN-γ含量(P＜0.05;P＜0.01)。【结论】AAP可协同LPS和PHA刺激小鼠脾脏淋巴细胞增殖,提高淋巴细胞表面CD4⁺/CD3⁺和CD8⁺/CD3⁺比值及淋巴细胞释放细胞因子的能力,进而提高机体免疫功能。

关键词: 茶树菇多糖(APP); 小鼠; 淋巴细胞; 免疫调节

Abstract: 【Objective】 This study was aimed to explore the effect of Agrocybe aegerita polysaccharid (AAP) on immunomodulatory of splenic lymphocytes in mice,in order to provide a basis for elucidating the action mechanism and developing the clinical application of AAP.【Method】 The splenic lymphocytes in mice were taken as the subjects,the MTT method was used to determine the safe concentration of AAP on lymphocytes,and the safe concentrations were selected for subsequent experiments.MTT assay was also used to detect the effect of different concentrations of AAP on the proliferation of splenic lymphocytes in mice.Three control groups of cell,lipopolysaccharide (LPS) and plant hemagglutinin (PHA) were also set at the same time to screen out AAP with the stronger proliferation ability on splenic lymphocytes in mice,flow cytometry was used to detect the effect of AAP on the values of CD4⁺/CD3⁺ and CD8⁺/CD3⁺ of T lymphocyte subtypes.ELISA method was used to determine the effect of AAP on the contents of interleukin-2 (IL-2),IL-4,tumor necrosis factor alpha (TNF-α) and gamma interferon (IFN-γ) in the supernatant of lymphocytes.【Result】 AAP concentration lower than 1 000 μg/mL had not any toxic effect on splenic lymphocytes in mice.Compared with control group,different doses of AAP could extremely significantly cooperate with LPS and PHA to stimulate lymphocyte proliferation (P＜0.01),125 and 250 μg/mL AAP could extremely significantly increase the values of CD4⁺/CD3⁺ and CD8⁺/CD3⁺ (P＜0.01),and significantly or extremely significantly increase the contents of IL-2,IL-4,TNF-α and IFN-γ secreted by lymphocytes (P＜0.05 or P＜0.01).【Conclusion】 AAP could cooperate with LPS and PHA to stimulate lymphocyte proliferation in spleen of mice,increase the values of CD4⁺/CD3⁺ and CD8⁺/CD3⁺ on the surface of lymphocytes and the ability of lymphocytes to release cytokines,thus improving the immune function of the body.

Key words: Agrocybe aegerita polysaccharid (AAP); mice; lymphocytes; immunoregulation

中图分类号:

S646.1

陆辉, 蔡高峰, 武彩红, 邱树磊, 袁橙, 陈晓兰, 刘芸, 覃凯莉. 茶树菇多糖对小鼠脾脏淋巴细胞免疫调节的影响[J]. 中国畜牧兽医, 2023, 50(5): 1828-1835.

LU Hui, CAI Gaofeng, WU Caihong, QIU Shulei, YUAN Chen, CHEN Xiaolan, LIU Yun, QIN Kaili. Effect of Agrocybe aegerita Polysaccharid on Immunomodulatory of Splenic Lymphocytes in Mice[J]. China Animal Husbandry and Veterinary Medicine, 2023, 50(5): 1828-1835.

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