IGFBP-3基因在两个品种鸡组织中的表达及其与生长性状的相关性分析

doi:10.16431/j.cnki.1671-7236.2020.07.023

摘要/Abstract

摘要： 为研究IGFBP-3基因表达与鸡生长性状的相关性，以生长速度差异较大的花山麻鸡和清远麻鸡两个黄羽肉鸡品种为研究素材，用实时荧光定量PCR法检测胚胎期和出雏后两个品种鸡胸肌和肝脏中IGFBP-3基因的表达规律，并将其与体重、胸肌重和肝脏重进行相关性分析。结果表明，在9胚龄时两个品种鸡胸肌和肝脏中均可检测到IGFBP-3基因表达，同一胚龄或日龄品种内组织间比较，胚胎期两个品种鸡胸肌IGFBP-3 mRNA表达量均高于肝脏，出雏后肝脏IGFBP-3 mRNA表达量迅速上升，胸肌IGFBP-3 mRNA表达量下降，两个品种鸡肝脏IGFBP-3 mRNA表达量均极显著高于胸肌（P<0.01）；组织中IGFBP-3 mRNA表达量与组织重量和体重相关研究表明，两个品种鸡肝脏IGFBP-3 mRNA表达量与其胸肌重、肝脏重和体重呈显著或极显著正相关（P<0.05；P<0.01），胸肌IGFBP-3 mRNA表达量与其胸肌重、肝脏重和体重均呈极显著负相关（P<0.01）。研究结果揭示，IGFBP-3 mRNA在鸡中的表达具有品种、年龄和组织特异性，出雏前肝脏并不是鸡产生IGFBP-3的主要器官，出雏后鸡IGFBP-3可能主要由肝脏合成，肝脏中IGFBP-3 mRNA水平差异是导致两个品种鸡出雏后体重和胸肌重差异的重要因素。

关键词: IGFBP-3基因; 鸡; 表达; 生长性状

Abstract: To observe the correlation between the expression of the IGFBP-3 gene and growth traits in chickens,the expression profiles of the IGFBP-3 gene of pectoral muscle and liver in two yellow-feathered broiler breeds (Huashan partridge chickens and Qingyuan partridge chickens) differing in growth rate were detected by Real-time quantitative PCR.Results showed that IGFBP-3 mRNA could be detected in pectoral muscle and liver at 9 embryonic day.The expression level of IGFBP-3 mRNA in pectoral muscle in both two chicken breeds was higher than that in liver at the same embryo age.After hatching,the expression level of IGFBP-3 mRNA in liver in two chicken breeds increased rapidly while the expression level of IGFBP-3 mRNA in pectoral muscle decreased.The expression level of IGFBP-3 mRNA in liver in both two chicken breeds was significantly higher than that in pectoral muscle at the same day old (P<0.01).The correlation analysis showed that in both two chicken breeds the mass of body,pectoral muscle and liver were significantly positively correlated with the expression level of IGFBP-3 mRNA in liver (P<0.05;P<0.01),and significantly negatively correlated with the expression level of IGFBP-3 mRNA in pectoral muscle(P<0.01).These results indicated that the expression level of the IGFBP-3 gene in chickens varied in breed,age and organ.The liver produces most IGFBP-3 after hatching,and the expression level of IGFBP-3 mRNA in liver might be important for the body mass and pectoral mass in chickens.

Key words: IGFBP-3 gene; chicken; expression; growth traits

中图分类号:

S831.2

单艳菊, 姬改革, 巨晓军, 章明, 屠云洁, 刘一帆, 邹剑敏, 束婧婷. IGFBP-3基因在两个品种鸡组织中的表达及其与生长性状的相关性分析[J]. 中国畜牧兽医, 2020, 47(7): 2181-2189.

SHAN Yanju, JI Gaige, JU Xiaojun, ZHANG Ming, TU Yunjie, LIU Yifan, ZOU Jianmin, SHU Jingting. Expression of IGFBP-3 Gene in Tissues of Two Chicken Breeds and Its Association with Growth Traits[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(7): 2181-2189.

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