虾青素对小鼠早期胚胎发育及相关基因表达的影响

doi:10.16431/j.cnki.1671-7236.2020.01.018

摘要/Abstract

摘要： 试验旨在研究培养液中添加虾青素（AX）对小鼠胚胎体外发育和相关基因表达的影响。试验选用23~25 g的ICR系雌性小鼠，利用超数排卵，取卵母细胞进行体外受精，受精1 h后将受精卵随机分组、分别移入浓度为0、5、10、25、50 μmol/L的虾青素培养液中进行体外发育培养，在培养24和96 h后分别观察并统计各组的卵裂率和囊胚率；体外培养至96 h后，对囊胚进行细胞计数，统计囊胚细胞数差异；提取各组囊胚总RNA，利用实时荧光定量PCR技术对各组胚胎的TGF-β和Bcl-2基因的相对表达量进行检测。结果显示，添加10 μmol/L虾青素能够改善小鼠胚胎体外发育环境且显著提高胚胎卵裂率和囊胚率（P<0.05）；显著提高囊胚的细胞数（P<0.05）；极显著提高TGF-β基因表达量（P<0.01）。结果表明，添加10 μmol/L虾青素有利于小鼠胚胎体外发育，可用于改善小鼠胚胎体外发育环境；TGF-β和Bcl-2基因很可能参与了胚胎发育过程中的相关调控。

关键词: 虾青素; 小鼠; 胚胎; 体外培养

Abstract: In present study,the effects of astaxanthin (AX) supplement in in vitro culture (IVC) medium on mouse embryo development and gene expression were determined.The female ICR strain mice (body weight 23-25 g) were super ovulated.The oocytes obtained from mouse oviduct were fertilized in vitro (IVF).The zygotes were divided randomly into 5 media suppled with different concentration of astaxanthin (0,5,10,25 and 50 μmol/L) for culture in vitro.Cultured for 24,96 h,the cleavage rate and blastocyst rate were analyzed.At 96 h,the cell number of blastocysts in each group was extracted.The total RNA of blastocysts in each group was abstracted,and relative expression amounts of TGF-β and Bcl-2 genes were determined by Real-time PCR.The results indicated that 10 μmol/L astaxanthin supplement could improve the in vitro development environment of mouse embryos and improve significantly embryo cleavage rate and blastocyst rate (P<0.05),increase cell number of blastocyst significantly (P<0.05),and increase expression amount of TGF-β gene extremely significantly (P<0.01).TGF-β and Bcl-2 genes were likely to be involved in the regulation of embryonic development.These results demonstrated that supplement of 10 μmol/L astaxanthin was beneficial to mouse embryo development in vitro,and could improve the developmental environment of mouse embryo.

Key words: astaxanthin; mouse; embryo; culture in vitro

中图分类号:

S814.6

阿拉法特·阿木拉提, 赵茜, 努日比娅姆·麦麦提托合提, 阿尔曼·海热, 刘国世, 阿布力孜·吾斯曼. 虾青素对小鼠早期胚胎发育及相关基因表达的影响[J]. 中国畜牧兽医, 2020, 47(1): 148-155.

ALAFAT·Amulati, ZHAO Xi, NURIBIYAMU·Maimaitituoheti, AERMAN·Haire, LIU GuoShi, ABULIZI·Wusiman. Effects of Astaxanthin on Mouse Implantation Embryos Development and Gene Expression[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(1): 148-155.

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