›› 2010, Vol. 37 ›› Issue (3): 123-126.

• 遗传繁育 • 上一篇    下一篇

延边黄牛PRKAG3基因第10外显子单核苷酸多态性及其与肉质性状的相关分析

李静1,2,曹阳1 ,张立春1,周国利1,3,于永生1,严昌国2,金海国1   

  1. (1.吉林省农业科学院畜牧分院, 公主岭 136100; 2.延边大学农学院, 龙井 133400;3.聊城大学生命科学学院, 聊城 252059)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2010-03-20 发布日期:2010-03-20
  • 通讯作者: 严昌国

Single Nucleotide Polymorphism in Yanbian Yellow Cattle Exon 10 of PRKAG3 Gene and its Association with Meat Quality Traits

LI Jing1,2,CAO Yang1,ZHANG Li-chun1,ZHOU Guo-li1,3,YU Yong-sheng1,YAN Chang-guo2,JIN Hai-guo1   

  1. (1.Animal Science Part of Jilin Province Academy of Agricultural Science, Gongzhuling 136100, China;2.College of Agronomy, Yanbian University, Longjing 133400, China; 3.College of Life Science, Liaocheng University, Liaocheng 252059, China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2010-03-20 Published:2010-03-20
  • Contact: YAN Chang-guo

摘要: 采用PCR-SSCP方法对120头延边黄牛PRKAG3基因的多态性进行检测,结果发现第10外显子存在C4738T突变,产生3种基因型。不同基因型与肉质性状均值差异显著性检验结果发现,基因型CT与a*24、b*24、油酸存在一定的负相关(P<0.05);与嫩度、谷氨酸、精氨酸、丙氨酸、缬氨酸和脯氨酸有一定的正相关(P<0.05);基因型CC与天冬氨酸存在一定的正相关(P<0.05)。

关键词: PRKAG3基因; 肉质性状; PCR-SSCP; 延边黄牛

Abstract: The polymorphisms of PRKAG3 gene were detected by PCR-SSCP method in 120 Yanbian Yellow Cattles.C4738T mutation was founded at exon 10.significance test of meat trait means for different genetypes showed:genotype CT was negatived correlation with a*24, b*24 and oleic acid (P<0.05);and was positived correlation with tenderness, Glu, Arg, Ala, Val and Pro(P<0.05);genotype CC was positived correlation with Asp(P<0.05).

Key words: PRKAG3 gene; meat quality traits; PCR-SSCP; Yanbian Yellow Cattle

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