中国畜牧兽医 ›› 2022, Vol. 49 ›› Issue (5): 1895-1907.doi: 10.16431/j.cnki.1671-7236.2022.05.030
史茜1,2, 徐新峰2, 李学文2, 肖媛媛2, 白梅花2, 张富春1
收稿日期:
2021-11-05
出版日期:
2022-05-05
发布日期:
2022-04-29
通讯作者:
张富春
E-mail:zfcxju@xju.edu.cn
作者简介:
史茜,E-mail:shiqian1985101518@163.com。
基金资助:
SHI Qian1,2, XU Xinfeng2, LI Xuewen2, XIAO Yuanyuan2, BAI Meihua2, ZHANG Fuchun1
Received:
2021-11-05
Online:
2022-05-05
Published:
2022-04-29
摘要: 多糖可促进免疫调节,具有多靶点、多功能和多因子效应,因此可广泛用作兽用疫苗免疫佐剂。多糖作为疫苗佐剂可提高免疫动物脾脏、胸腺和法氏囊等免疫器官指数,增强巨噬细胞吞噬活性及抗原递呈能力,增强NK细胞杀伤能力,促进树突细胞的成熟和分化,增强机体对抗原的摄取能力,影响T细胞亚群的种类和数量,改变Th1、Th2型免疫应答反应的类型,促进B淋巴细胞增殖,刺激细胞因子及抗体的产生等。而多糖作为配体通过与Toll样受体(Toll-like receptor,TLR)、甘露糖受体(mannose receptor,MR)、C型凝集素受体(C-type lectins receptor,CLR)、清道夫受体(scavenger receptor,SR)等受体分子结合,激活下游信号通路,进而发挥上述免疫活性。文章主要从上述几方面对多糖作为兽用疫苗佐剂的作用机制进行阐述,以期为多糖的进一步开发和应用提供参考。
中图分类号:
史茜, 徐新峰, 李学文, 肖媛媛, 白梅花, 张富春. 多糖作为兽用疫苗佐剂作用机制的研究进展[J]. 中国畜牧兽医, 2022, 49(5): 1895-1907.
SHI Qian, XU Xinfeng, LI Xuewen, XIAO Yuanyuan, BAI Meihua, ZHANG Fuchun. Research Progress on the Mechanism of Polysaccharide as Adjuvant of Veterinary Vaccine[J]. China Animal Husbandry and Veterinary Medicine, 2022, 49(5): 1895-1907.
[1] 苏建青,刘学彬,张晓云,等.植物多糖免疫调节功能研究进展[J].湖北农业科学,2018,57(11):10-13. SU J Q,LIU X B,ZHANG X Y,et al.Research progress in immunomodulating functions of plant polysaccharide[J].Hubei Agricultural Sciences,2018,57(11):10-13.(in Chinese) [2] 杨 琳,傅哲彦,吕正兵,等.免疫佐剂分类及作用机制[J].中国生物工程杂志,2019,39(5):114-119. YANG L,FU Z Y,LYU Z B,et al.Classification and mechanism of immunoadjuvants[J].China Biotechnology,2019,39(5):114-119.(in Chinese) [3] 崔倩倩,周志勇,刘朝奇,等.植物多糖作为免疫佐剂的研究进展[J].时珍国医国药,2015,26(4):970-972. CUI Q Q,ZHOU Z Y,LIU C Q,et al.Development in plant-derived polysaccharides as immune adjuvant research[J].Lishizhen Medicine and Materia Medica,2015,26(4):970-972.(in Chinese) [4] 陈亚楠,李美锋,董艳敏,等.中药多糖免疫调节作用研究进展[J].国际中医中药杂志,2016,38(9):855-858. CHEN Y N,LI M F,DONG Y M,et al.The research progress in immune regulation of polysaccharides of traditional Chinese medicine[J].International Journal of Traditional Chinese Medicine,2016,38(9):855-858.(in Chinese) [5] 郭林霞,马可为,冯志华,等.枣多糖对免疫抑制蛋雏鸡生长性能和免疫指标的影响[J].中国畜牧兽医,2021,48(9):3264-3272. GUO L X,MA K W,FENG Z H,et al.Effects of jujube polysaccharide on growth performance and immune indexes of immunosuppressive egg-type chicks[J].China Animal Husbandry & Veterinary Medicine,2021,48(9):3264-3272.(in Chinese) [6] 孔晨先,肖 波,柳全文,等.山药多糖对动物机体免疫调节作用的Meta分析[J].食品研究与开发,2021,42(19):206-213. KONG C X,XIAO B,LIU Q W,et al.Meta-analysis of the immunomodulatory effect of Chinese yam (Dioscorea opposita rhizoma) polysaccharides on the animal body[J].Food Research and Development,2021,42(19):206-213.(in Chinese) [7] 李 钦,胡继宏,高 博,等.黄芪多糖在免疫调节方面的最新研究进展[J].中国实验方剂学杂志,2017,23(2):199-206. LI Q,HU J H,GAO B,et al.Advances on immunoregulation effect of Astragalus polysaccharides[J].Chinese Journal of Experimental Prescriptions,2017,23(2):199-206.(in Chinese) [8] ENGEL A L,SUN G C,GAD E,et al.Protein-bound polysaccharide activates dendritic cells and enhances OVA-specific T cell response as vaccine adjuvant[J].Immunobiology,2013,218(12):1468-1476. [9] 吴鑫平,李 佳,郝艳艳,等.植物多糖对巨噬细胞的调控及机制研究进展[J].细胞与分子免疫学杂志,2019,35(5):473-478. WU X P,LI J,HAO Y Y,et al.Research progress on regulation and mechanism of plant polysaccharides on macrophages[J].Chinese Journal of Cellular and Molecular Immunology,2019,35(5):473-478.(in Chinese) [10] 秦延军,杨树宝,孙 朋,等.中药多糖对巨噬细胞和树突状细胞免疫调节作用的研究进展[J].中国兽药杂志,2018,52(2):72-77. QIN Y J,YANG S B,SUN P,et al.Effects of polysaccharides in Chinese medicine on the immunomodulator effect of macrophage and dendritic cell[J].Chinese Journal of Veterinary Drug,2018,52(2):72-77.(in Chinese) [11] 杜海东,邢媛媛,金 晓,等.植物多糖对动物免疫细胞的影响及调节机制研究进展[J].饲料研究,2021,44(2):117-121. DU H D,XING Y Y,JIN X,et al.Research progress on effect of plant polysaccharides on animal immune cells and the action mechanism[J].Feed Research,2021,44(2):117-121.(in Chinese) [12] YANG S,DONG W,LI G,et al.A recombinant vaccine of Riemerella anatipestifer OmpA fused with duck IgY Fc and Schisandra chinensis polysaccharide adjuvant enhance protective immune response[J].Microbial Pathogenesis,2019,136:103707. [13] 蔡 琨,卢芳国,杨 娟,等.仙茅多糖作为流感疫苗佐剂对小鼠巨噬细胞的免疫增强作用[J].中华中医药杂志,2018,33(4):1344-1347. CAI K,LU F G,YANG J,et al.Immunological enhancement effects of Curculigo orchioides polysaccharide as adjuvant of influenza vaccine on mouse macrophage[J].Chinese Journal of Traditional Chinese Medicine,2018,33(4):1344-1347.(in Chinese) [14] 卞 娅,葛广波,丁 侃.中药多糖抗病毒机制研究进展[J].上海中医药大学学报,2021,35(5):89-96. BIAN Y,GE G B,DING K.Research advance on antiviral mechanism of herbal polysaccharides[J].Academic Journal of Shanghai University of Traditional Chinese Medicine,2021,35(5):89-96.(in Chinese) [15] SUN Y,GUO M,FENG Y,et al.Effect of ginseng polysaccharides on NK cell cytotoxicity in immunosuppressed mice[J].Experimental and Therapeutic Medicine,2016,12(6):3773-3777. [16] DEL ZOTTO G,MARCENARO E,VACCA P,et al.Markers and function of human NK cells in normal and pathological conditions[J].Cytometry Part B-Clinical Cytometry,2017,92(2):100-114. [17] 谢红艳,张文伟,林丹义,等.黄芪多糖佐剂对脾脏NK细胞及NKT细胞的作用[J].热带医学杂志,2013,13(6):729-732. XIE H Y,ZHANG W W,LIN D Y,et al.The role of NK and NKT cells in APS adjuvant induced immunologic effect[J].Journal of Tropical Medicine,2013,13(6):729-732.(in Chinese) [18] LI J,LI J,ZHANG F.The immunoregulatory effects of Chinese herbal medicine on the maturation and function of dendritic cells[J].Journal of Ethnopharmacology,2015,171:184-195. [19] FENG H,FAN J,QIU H,et al.Chuanminshen violaceum polysaccharides improve the immune responses of foot-and-mouth disease vaccine in mice[J].International Journal of Biological Macromolecules,2015,78:405-416. [20] DU X,ZHAO B,LI J,et al.Astragalus polysaccharides enhance immune responses of HBV DNA vaccination via promoting the dendritic cell maturation and suppressing Treg frequency in mice[J].International Immunopharmacology,2012,14(4):463-470. [21] KWAK M,YU K,LEE P C,et al.Rehmannia glutinosa polysaccharide functions as a mucosal adjuvant to induce dendritic cell activation in mediastinal lymphnode[J].International Journal of Biological Macromolecules,2018,120(Pt B):1618-1623. [22] 彭娟娟,李向敏,王 涓,等.食药用真菌多糖对肿瘤免疫逃逸调节作用机制研究进展[J].微生物学报,2021,61(9):2594-2606. PENG J J,LI X M,WANG J,et al.Research progress on regulating tumor immune escape mechanism of edible and medicinal fungal polysaccharides[J].Acta Microbiologica Sinica,2021,61(9):2594-2606.(in Chinese) [23] 罗 霞,胡明华,马方励,等.中药多糖对免疫抑制小鼠T细胞亚群影响的差异研究[J].实用医药杂志,2016,33(7):621-624. LUO X,HU M H,MA F L,et al.Differential study on the effect of traditional Chinese medicine polysaccharides on T cell subsets in immunosuppressive mice[J].Journal of Practical Medicine,2016,33(7):621-624.(in Chinese) [24] 邵 珊,王贵平,李春玲,等.黄芪多糖和白花蛇舌草多糖对猪繁殖与呼吸综合征灭活疫苗免疫猪T细胞亚群及抗体水平的影响[J].中国兽医学报,2011,31(8):1196-1199. SHAO S,WANG G P,LI C L,et al.Effects of APS and OPS on T subpopulations in the immune response to Porcine reproductive and respiratory syndrome inactived virus vaccine[J].Chinese Journal of Veterinary Science,2011,31(8):1196-1199.(in Chinese) [25] WANG D,YANG Y,LI J,et al.Enhancing immune responses to inactivated Foot-and-mouth virus vaccine by a polysaccharide adjuvant of aqueous extracts from Artemisia rupestris L[J].Journal of Veterinary Science,2021,22(3):e30. [26] ZHAO B,LIAN J,WANG D,et al.Evaluation of aqueous extracts of Cistanche deserticola as a polysaccharide adjuvant for seasonal influenza vaccine in young adult mice[J].Immunology Letters,2019,213(5):1-8. [27] ZHANG W,CHENG N,WANG Y,et al.Adjuvant activity of PCP-Ⅱ a polysaccharide from Poria cocos on a whole killed Rabies vaccine[J].Virus Research,2019,270(8):197638. [28] FENG H,MCDONOUGH S P,FAN J,et al.Phosphorylated Radix cyathulae officinalis polysaccharides act as adjuvant via promoting dendritic cell maturation[J].Molecules,2017,22(1):106. [29] LIU K,YIN Y,ZHANG J,et al.Polysaccharide PCP-Ⅰ isolated from Poria cocos enhances the immunogenicity and protection of an anthrax protective antigen-based vaccine[J].Human Vaccines & Immunotherapeutics,2020,16(7):1699-1707. [30] 李群芳,武 蕾,姜宁鹏.细胞因子的研究概况[J].山东畜牧兽医,2019,40(11):67-69. LI Q F,WU L,JIANG N P.Overview of cytokine research[J].Shandong Animal Husbandry and Veterinary,2019,40(11):67-69.(in Chinese) [31] 赵 怡,文露婷,黄 姻,等.山豆根多糖对罗非鱼生长性能和免疫功能的影响[J].中国畜牧兽医,2021,48(10):3635-3643. ZHAO Y,WEN L T,HUANG Y,et al.Effects of Sophora subprosrate polysaccharide on growth performance and immune function of Oreochromis niloticus[J].China Animal Husbandry & Veterinary Medicine,2021,48(10):3635-3643.(in Chinese) [32] LIU Y,ZHANG S,ZHANG F,et al.Adjuvant activity of Chinese herbal polysaccharides in inactivated veterinary rabies vaccines[J].International Journal of Biological Macromolecules,2012,50(3):598-602. [33] LIU C,LUO J,XUE R Y,et al.The mucosal adjuvant effect of plant polysaccharides for induction of protective immunity against Helicobacter pylori infection[J].Vaccine,2019,37(8):1053-1061. [34] HWANG S H,SHIN M S,YOON T J,et al.Immunoadjuvant activity in mice of polysaccharides isolated from the leaves of Panax ginseng C.A.Meyer[J].International Journal of Biological Macromolecules,2018,107(Pt B):2695-2700. [35] LIU Z,ZHU T,HE J,et al.Adjuvanticity of Ganoderma lucidum polysaccharide liposomes on Porcine circovirus type-Ⅱin mice[J].International Journal of Biological Macromolecules,2019,141:1158-1164. [36] 梁 艳,黄 恋,常海艳,等.人参多糖对新甲型H1N1流感病毒灭活疫苗的免疫增强作用[J].激光生物学报,2012,21(1):36-41. LIANG Y,HUANG L,CHANG H Y,et al.Antibody responses induced by inactivated new Influenza A virus(H1N1) vaccine with ginseng polysaccharide as an adjuvant[J].Acta Laser Biology Sinica,2012,21(1):36-41.(in Chinese) [37] 孟秀彦,楚遵锋,张静静,等.银杏叶多糖对传染性法氏囊超强毒灭活疫苗的免疫调节作用[J].中国兽医学报,2019,39(4):640-645. MENG X Y,CHU Z F,ZHANG J J,et al.Immunomodulatory functions of Ginkgo biloba leaves polysaccharide on vvIBDV vaccine[J].Chinese Journal of Veterinary Science,2019,39(4):640-645.(in Chinese) [38] LI P,WANG F.Polysaccharides:Candidates of promising vaccine adjuvants[J].Drug Discoveries & Therapeutics,2015,9(2):88-93. [39] 汤小芳,刘 想,胡美华,等.多糖对免疫系统调控的研究进展[J].食品工业科技,2018,39(9):325-331. TANG X F,LIU X,HU M H,et al.Research progress on the immune regulation of polysaccharides related to immune system[J].Science and Technology of Food Industry,2018,39(9):325-331.(in Chinese) [40] 朱雪坤,杨日芳,孟艳秋.Toll样受体4配体的研究进展[J].中国药理学与毒理学杂志,2016,30(4):389-396. ZHU X K,YANG R F,MENG Y Q.Research progress in ligands of Toll-like receptor 4[J].Chinese Journal of Pharmacology and Toxicology,2016,30(4):389-396.(in Chinese) [41] ZHANG X,QI C,GUO Y,et al.Toll-like receptor 4-related immunostimulatory polysaccharides:Primary structure,activity relationships,and possible interaction models[J].Carbohydrate Polymers,2016,149:186-206. [42] 杨斯锦,潘 超,朱 力,等.Toll样受体2激动剂在疫苗研发中的研究进展[J].安徽农业科学,2020,48(7):7-10. YANG S J,PAN C,ZHU L.Research progress of Toll-like receptor 2 agonists in vaccine development[J].Journal of Anhui Agricultural Sciences,2020,48(7):7-10.(in Chinese) [43] AHMADISHOAR S,KARIMINIK A.Toll-like receptor 2 and its roles in immune responses against Legionella pneumophila[J].Life Sciences,2017,188:158-162. [44] 汲晨锋,陈锦瑞,张子依.植物多糖受体研究进展[J].中国药学杂志,2019,54(21):1766-1772. JI C F,CHEN J R,ZHANG Z Y.Research progress of receptors of plant polysaccharide[J].Chinese Pharmaceutical Journal,2019,54(21):1766-1772.(in Chinese) [45] BROWN G D,WILLMENT J A,WHITEHEAD L.C-type lectins in immunity and homeostasis[J].Nature Reviews Immunology,2018,18(6):374-389. [46] 徐旋里,周建英.糖类识别受体:甘露糖受体[J].国际病理科学与临床杂志,2006,26(4):351-354. XU X L,ZHOU J Y.Carbohydrate recognition receptor:Mannose receptor[J].International Journal of Pathology and Clinical Medicine,2006,26(4):351-354.(in Chinese) [47] 刘小玲,曾令兵.甘露糖受体结构、功能、表达和应用研究的新进展[J].水产学杂志,2013,26(1):54-59. LIU X L,ZENG L B.A review of the research advancement of structure,function,expression and application of mannose receptor[J].Chinese Journal of Fisheries,2013,26(1):54-59.(in Chinese) [48] VAN DER ZANDE H J P,NITSCHE D,SCHLAUTMANN L,et al.The mannose receptor:From endocytic receptor and biomarker to regulator of (meta) inflammation[J].Frontiers in Immunology,2021,14(12):765034. [49] JIN Y,LI P,WANG F.β-glucans as potential immunoadjuvants:A review on the adjuvanticity,structure-activity relationship and receptor recognition properties[J].Vaccine,2018,36(35):5235-5244. [50] HOU H,GUO Y,CHANG Q,et al.C-type lectin receptor:Old friend and new player[J].Medicinal Chemistry,2017,13(6):536-543. [51] WILLMENT J A,BROWN G D.C-type lectin receptors in antifungal immunity[J].Trends in Microbiology,2008,16(1):27-32. [52] SAIJO S,IKEDA S,YAMABE K,et al.Dectin-2 recognition of alpha-mannans and induction of Th17 cell differentiation is essential for host defense against Candida albicans[J].Immunity,2010,32(5):681-691. [53] VORUP-JENSEN T,JENSEN R K.Structural immunology of complement receptors 3 and 4[J].Frontiers in Immunology,2018,9:2716. [54] FERREIRA S S,PASSOS C P,MADUREIRA P,et al.Structure-function relationships of immunostimulatory polysaccharides:A review[J].Carbohydrate Polymers,2015,132:378-396. [55] RYU S,HOWLAND A,SONG B,et al.Scavenger receptor class A to E involved in various cancers[J].Chonnam Medical Journal,2020,56(1):1-5. [56] SU C H,LU M K,LU T J,et al.A (1→6)-branched (1→4)-β-d-glucan from Grifola frondosa inhibits lipopolysaccharide-induced cytokine production in RAW264.7 macrophages by binding to TLR2 rather than dectin-1 or CR3 receptors[J].Journal Natural Products,2020,83(2):231-242. [57] WEI W,XIAO H T,BAO W R,et al.TLR-4 may mediate signaling pathways of Astragalus polysaccharide RAP induced cytokine expression of RAW264.7 cells[J].Journal of Ethnopharmacology,2016,179:243-252. [58] GUO L,XIE J,RUAN Y,et al.Characterization and immunostimulatory activity of a polysaccharide from the spores of Ganoderma lucidum[J].International Immunopharmacology,2009,9(10):1175-1182. [59] RASHID Z M,MORMANN M,STECKHAN K,et al.Polysaccharides from lichen Xanthoria parietina:1,4/1,6-α-D-glucans and a highly branched galactomannan with macrophage stimulating activity via dectin-2 activation[J].International Journal of Biological Macromolecules,2019,134:921-935. [60] LIAO W,LUO Z,LIU D,et al.Structure characterization of a novel polysaccharide from Dictyophora indusiata and its macrophage immunomodulatory activities[J].Journal of Agricultural and Food Chemistry,2015,63(2):535-544. [61] DENG C,FU H,SHANG J,et al.Dectin-1 mediates the immunoenhancement effect of the polysaccharide from Dictyophora indusiata[J].International Journal of Biological Macromolecules,2018,109:369-374. [62] ZHAN Q,WANG Q,LIN R,et al.Structural characterization and immunomodulatory activity of a novel acid polysaccharide isolated from the pulp of Rosa laevigata michx fruit[J].International Journal of Biological Macromolecules,2020,145:1080-1090. [63] LEE J,LI C,SURAYOT U,et al.Molecular structures,chemical properties and biological activities of polysaccharide from Smilax glabra rhizome[J].International Journal of Biological Macromolecules,2018,120(Pt B):1726-1733. [64] LEE J B,TANIKAWA T,HAYASHI K,et al.Characterization and biological effects of two polysaccharides isolated from Acanthopanax sciadophylloides[J].Carbohydrate Polymers,2015,116:159-166. [65] HUANG Z,ZENG Y J,CHEN X,et al.A novel polysaccharide from the roots of Millettia speciosa champ:Preparation,structural characterization and immunomodulatory activity[J].International Journal of Biological Macromolecules,2020,145:547-557. [66] JIN Y,ZHANG Y,WAN C,et al.Immunomodulatory activity and protective effects of polysaccharide from Eupatorium adenophorum leaf extract on highly pathogenic H5N1 influenza infection[J].Evidence-Based Complementary and Alternative Medicine,2013,2013:194976. [67] KIM H W,SHIN M S,LEE S J,et al.Signaling pathways associated with macrophage-activating polysaccharides purified from fermented barley[J].International Journal of Biological Macromolecules,2019,131:1084-1091. [68] MINATO K I,LAAN L C,VAN DIE I,et al.Pleurotus citrinopileatus polysaccharide stimulates anti-inflammatory properties during monocyte-to-macrophage differentiation[J].International Journal of Biological Macromolecules,2019,122:705-712. [69] DONG Z,ZHANG M,LI H,et al.Structural characterization and immunomodulatory activity of a novel polysaccharide from Pueraria lobata (Willd.) Ohwi root[J].International Journal of Biological Macromolecules,2020,154:1556-1564. [70] LI W J,TANG X F,SHUAI X X,et al.Mannose receptor mediates the immune response to Ganoderma atrum polysaccharides in macrophages[J].Journal of Agricultural and Food Chemistry,2017,65(2):348-357. |
[1] | 钟粤韵, 杨舒展, 陈非玥, 陆智儿, 李冰心, 李婉雁, 田允波, 黄运茂, 许丹宁, 曹楠. LPS通过ROS/p38/MAPK信号通路对鹅胚肝细胞凋亡的影响[J]. 中国畜牧兽医, 2023, 50(6): 2224-2232. |
[2] | 汪阳阳, 秦士贞, 唐德富, 邵玉新, 史兆国, 王铮, 李星, 赵东栋. 锌、乳酸杆菌对感染沙门氏菌乳鸽免疫及抗氧化功能的影响[J]. 中国畜牧兽医, 2023, 50(6): 2276-2285. |
[3] | 韩冰, 汤思淇, 朱大帷, 李海玲, 冯亚莉, 苏超凡, 徐艺娜, 冷昊钰, 王永涛, 张月美, 太晓春, 张莹. 新型疫苗佐剂研究进展[J]. 中国畜牧兽医, 2023, 50(6): 2460-2467. |
[4] | 刘鹏, 李静静, 乔彦良, 朱连勤, 朱风华. 黄芩多糖的提取及结构表征[J]. 中国畜牧兽医, 2023, 50(6): 2518-2530. |
[5] | 陆辉, 蔡高峰, 武彩红, 邱树磊, 袁橙, 陈晓兰, 刘芸, 覃凯莉. 茶树菇多糖对小鼠脾脏淋巴细胞免疫调节的影响[J]. 中国畜牧兽医, 2023, 50(5): 1828-1835. |
[6] | 乔宏兴, 张菡, 赵圣振, 孙彦婷, 张晓静, 康宇, 边传周. 发酵杜仲叶对肉鸡生产性能、抗氧化性能和免疫相关基因表达的影响[J]. 中国畜牧兽医, 2023, 50(5): 1845-1854. |
[7] | 谢华亮, 雷小文, 李建军, 刘珍妮, 钟云平, 谭东海, 张强. 蚯蚓液对105~175日龄蛋鸡产蛋性能、血清生化指标、抗氧化指标及免疫相关指标的影响[J]. 中国畜牧兽医, 2023, 50(5): 1899-1906. |
[8] | 姜海军, 王丹, 郭禹, 韦莉, 侯磊, 王菁, 朱珊珊, 刘爵. 猪圆环病毒3型Cap重组鞭毛蛋白融合表达及其免疫原性研究[J]. 中国畜牧兽医, 2023, 50(5): 2044-2052. |
[9] | 邵永斌, 郑一闯, 张竞怡, 魏琳颖, 田勇, 孙国结, 刘雨然, 罗燕, 谷新利. 益母草碱对LPS诱导小鼠子宫内膜炎的抗炎作用研究[J]. 中国畜牧兽医, 2023, 50(5): 2149-2155. |
[10] | 胡红莲, 李超, 陈利青, 宋利文, 赵濛, 张剑霞, 王典, 高民. β-胡萝卜素对奶牛泌乳高峰期产乳性能及血常规指标、血清生化和免疫指标的影响[J]. 中国畜牧兽医, 2023, 50(4): 1375-1384. |
[11] | 张哲玮, 代小童, 韦祖丹, 陈云鹏, 甘晶, 贝为成. 猪多杀性巴氏杆菌OmpW、TbpA蛋白的原核表达及免疫原性研究[J]. 中国畜牧兽医, 2023, 50(4): 1489-1498. |
[12] | 刘家兴, 韩雪英, 张鑫茹, 邓嘉昕, 姚伦广, 刘阳坤. 携带猪流行性腹泻病毒抗原表位的铁蛋白纳米颗粒制备与免疫原性分析[J]. 中国畜牧兽医, 2023, 50(4): 1567-1574. |
[13] | 张国庆, 姜宇航, 张爽, 李乐天, 郝嘉翼, 陈竞, 邹万成, 李昌, 李太元, 高旭, 金宁一. 猪δ冠状病毒RBD蛋白多肽抗体的制备与鉴定[J]. 中国畜牧兽医, 2023, 50(4): 1575-1583. |
[14] | 郭冰玉, 李莉, 李趁, 孔祥会. Mpeg1在病原清除中的作用及分子机制[J]. 中国畜牧兽医, 2023, 50(4): 1584-1592. |
[15] | 姚家威, 黄钰洁, 陈志胜, 王丙云, 张晖. α-酮戊二酸二甲酯预处理对犬脂肪源间充质干细胞免疫调节能力的影响[J]. 中国畜牧兽医, 2023, 50(4): 1642-1652. |
阅读次数 | ||||||
全文 |
|
|||||
摘要 |
|
|||||