急性冷刺激后巴马猪背膘和腹股沟皮下脂肪组织的脂质组比较

doi:10.16431/j.cnki.1671-7236.2020.09.008

中国畜牧兽医 ›› 2020, Vol. 47 ›› Issue (9): 2759-2766.doi: 10.16431/j.cnki.1671-7236.2020.09.008

急性冷刺激后巴马猪背膘和腹股沟皮下脂肪组织的脂质组比较

潘建飞¹, 郑千涛², 陶聪¹, 赵建国², 王彦芳¹

1. 中国农业科学院北京畜牧兽医研究所, 北京 100193;
2. 中国科学院动物研究所, 北京 100101

收稿日期:2020-02-20 出版日期:2020-09-20 发布日期:2020-09-16
通讯作者: 王彦芳 E-mail:wangyanfang@caas.cn
作者简介:潘建飞(1988-),男,甘肃张掖人,博士生,研究方向:动物基因与细胞工程,E-mail:pjf124@126.com
基金资助:
国家自然科学基金（31672387）；中央级公益性科研院所基本科研业务费专项资金（2020-YWF-YB-04）；中国农业科学院科技创新工程（ASTIP-IAS05）

Comparison of Lipid Profiles of Backfat and Inguinal Subcutaneous White Adipose Tissue from Acute Cold-treated Bama Pigs

PAN Jianfei¹, ZHENG Qiantao², TAO Cong¹, ZHAO Jianguo², WANG Yanfang¹

1. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China;
2. Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China

Received:2020-02-20 Online:2020-09-20 Published:2020-09-16

摘要/Abstract

摘要： 本研究旨在获得巴马猪皮下脂肪组织（包括背膘（backfat）和腹股沟皮下脂肪组织（inguinal subcutaneous white adipose tissue，iWAT））在急性冷刺激（4 ℃、4 h）后其脂质组成应答的差异。正常饲喂的6月龄巴马公猪在急性冷刺激处理后进行屠宰，收集背膘和腹股沟皮下脂肪组织，利用液相二级质谱（LC-MS/MS）高通量脂质组学检测技术分析其脂质组成。结果表明，在巴马猪皮下脂肪组织中，中性脂类、游离脂肪酸、磷脂类和鞘脂类4大类脂质含量差异巨大，其中含量最高的为中性脂类，在腹股沟皮下脂肪组织和背膘中分别占4大脂类总和的97.43%和98.53%；含量最低的为鞘脂类，在腹股沟皮下脂肪组织和背膘中所占比例分别为0.10%和0.07%。在16亚类脂质组成中含量最高的脂质为甘油三酯，在腹股沟皮下脂肪组织和背膘分别占16亚类脂质总和的95.97%和97.33%；含量最低的脂质为磷脂酸，在腹股沟皮下脂肪组织和背膘中所占比例分别为3.98E-04%和1.13E-04%。主成分分析表明，背膘和腹股沟皮下脂肪组织在急性冷刺激后脂质组成存在差异。以∣差异倍数∣>1.5和P<0.05为标准筛选，共获得18种差异显著的脂类，其中16种脂类在背膘中含量显著低于腹股沟皮下脂肪组织，其中包括14种甘油三酯、DAG32：1（16：1/16：0）和PE40：6p；只有2种脂质（CL74：8（18：2）和TAG54：3（16：0））在背膘中的含量显著高于腹股沟皮下。巴马猪皮下脂肪主要脂质组成为甘油三酯；急性冷刺激后，背膘和腹股沟皮下脂肪组织对急性冷刺激存在不同的应答。

关键词: 脂质组; 背膘; 腹股沟皮下脂肪组织; 急性冷刺激; 巴马猪

Abstract: This experiment aimed to study the difference of lipid metabolism between backfat and inguinal subcutaneous white adipose tissue (iWAT) after acute cold exposure (4 ℃ for 4 h).The LC-MS/MS based lipidomics of backfat and iWAT from chow diet-fed and cold-treated six-month-old male Bama pigs were profiled.The results showed that the content of 4 lipids classes,including neutral lipids,free fatty acids,phospholipids and sphingolipids were varies greatly in iWAT of Bama pigs.The highest lipid class in both iWAT and backfat was neutral lipids,which accounted for 97.43% and 98.53% respectively;The lowest lipid class was sphingolipids,which accounted for 0.10%and 0.07% in iWAT and backfat,respectively.Among 16 subclass lipids,the highest content in iWAT and backfat was TAG,which accounted for 95.97% and 97.33%,respectively,while the lowest content was PA,which accounted for 3.98E-04% and 1.13E-04% in iWAT and backfat,respectively.PCA analysis showed that backfat and iWAT could be separated clearly,which indicated the distinct changes in lipid composition of both fat tissues.And 18 different lipid species were determined using a criteria of fold change>1.5 and P<0.05,the results showed that 16 lipid species were significantly downregulated in backfat,including 14 TAGs,DAG32:1 (16:1/16:0) and PE40:6p.Two lipid species,including CL74:8(18:2) and TAG54:3 (16:0),were observed to be upregulated in backfat.The above results indicated that TAG was the main content of iWAT in Bama pigs,the backfat and iWAT had different physiological responses for acute cold stimulation.

Key words: lipidomics; backfat; inguinal subcutaneous adipose tissue; acute cold exposure; Bama pigs

中图分类号:

S858.28

潘建飞, 郑千涛, 陶聪, 赵建国, 王彦芳. 急性冷刺激后巴马猪背膘和腹股沟皮下脂肪组织的脂质组比较[J]. 中国畜牧兽医, 2020, 47(9): 2759-2766.

PAN Jianfei, ZHENG Qiantao, TAO Cong, ZHAO Jianguo, WANG Yanfang. Comparison of Lipid Profiles of Backfat and Inguinal Subcutaneous White Adipose Tissue from Acute Cold-treated Bama Pigs[J]. China Animal Husbandry and Veterinary Medicine, 2020, 47(9): 2759-2766.

参考文献

[1] 杨公社.猪生产学[M].北京:中国农业出版社,2012. YANG G S.Swine Production[M].Beijing:China Agriculture Press,2012.(in Chinese)
[2] 陈海燕,李江波,徐燕云,等.杂种猪胴体性状的通径分析[J].上海交通大学学报(农业科学版),2003,21(1):51-53. CHENG H Y,LI J B,XU Y Y,et al.Path analysis for the carcass traits of crossbreeding pigs[J].Journal of Shanghai Jiaotong University (Agricutural Science),2003,21(1):51-53.(in Chinese)
[3] 汪秀星,陈杰,刘红林.猪肌内脂肪沉积相关基因的差异显示反转录PCR鉴定[A].第十五次全国动物遗传育种学术讨论会[C].2009. WANG X X,CHEN J,LIU H L.Differences in genes associated with fat deposition in porcine muscle revealed reverse transcription PCR identification[A].The 15th Chinese National Conference on Animal Genetics and Breeding[C].2009.(in Chinese)
[4] PATIENCE J F,ROSSONI-SERAO M C,GUTIERREZ N A,et al.A review of feed efficiency in swine:Biology and application[J].Journal of Animal Husbandry and Biotechnology,2015,6(4):369-377.
[5] WANG W,SEALE P.Control of brown and beige fat development[J].Nature Reviews Molecular Cell Biology,2016,17:691-702.
[6] 江书忠.猪脂肪沉积关键基因的筛选及锌指蛋白KLF13的功能研究[D].武汉:华中农业大学,2014. JIANG S Z.Screening of key genes related to porcine fat deposition and functional study of zinc finger protein KLF13[D].Wuhan:Huazhong Agricultural University,2014.(in Chinese)
[7] PAN J,TAO C,AO C,et al.Adipose lipidomics and RNA-Seq analysis revealed the enhanced mitochondrial function in UCP1 knock-in pigs[J].Biochimica et Biophysica Acta.Molecular and Cell Biology of Lipids,2019,1864(10):1375.
[8] LIN J,CAO C,TAO C,et al.Cold adaptation in pigs depends on UCP3 in beige adipocytes[J].Journal of Molecular Cell Biology,2017,9(5):364-375.
[9] 周丽,陈宏权.皖南花猪的背膘厚度与胴体组成之相关分析[J].畜牧与兽医,2009,1:59-61. ZHOU L,CHENG H Q.Correlation analysis of backfat thickness and carcass composition in Wannan hua pigs[J].Animal Husbandry and Veterinary Medicine,2009,1:59-61.(in Chinese)
[10] 李青芝,王滔,李明洲,等.猪皮下与内脏脂肪组织mRNA转录组的构建与差异分析[J].四川农业大学学报,2013,31(1):65-70. LI Q Z,WANG T,LI M Z,et al.Identification of differential expressed transcripts between porcine subcutaneous and visceral adipose tissues[J].Journal of Sichuan Agricultural University,2013,31(1):65-70.(in Chinese)
[11] HAN X L.Lipidomics for studying metabolism[J].Nature Reviews Endocrinology,2016,12(11),668-679.
[12] LAMS M,WANGZ H,LI J,et al.Sequestration of polyunsaturated fatty acids in membrane phospholipids of Caenorhabditis elegans dauer larva attenuates eicosanoid biosynthesis for prolonged survival[J].Redox Biology,2017,12:967-977.
[13] WENK M R.The emerging field of lipidomics[J].Nature Reviews Drug Discovery,2005,4(7):594-610.
[14] EVA C,MICHAL H,MIROSLAV L.Nontargeted lipidomic characterization of porcine organs using hydrophilic interaction liquid chromatography and off-line two-dimensional liquid chromatography-electrospray ionization mass spectrometry[J].Lipids,2013,48(9):915-928.
[15] JAMESON J L,KOPP P.Chapter 4:Application of genetics in endocrinology[J].Endocrinology:Adult and Pediatric (Seventh Edition),2016,41-68.e8.
[16] MORITA S Y,TSKUJI T,TERADA T.Protocols for enzymatic fluorometric assays to quantify phospholipid classes[J].International Journal of Molecular Sciences,2020,21(3):1032.
[17] UMANO G R,PISTONE C,TONDINA E,et al.Pediatric obesity and the immune system[J].Frontiers in Pediatrics,2019,7:487.
[18] IBRAHIM M M.Subcutaneous and visceral adipose tissue:Structural and functional differences[J].Obesity Reviews,2010,11(1):11-18.
[19] ROSENWALD M,PERDIKARI A,RULICKE T,et al.Bidirectional interconversion of brite and whiteadi-pocytes[J].Nature Cell Biology,2013,15(6):659-667.
[20] SCHWEIZER S,LIEBISCH G,OECKL J,et al.The lipidome of primary murine white,brite,and brown adipocytes-Impact of beta-adrenergic stimulation[J].PLoS Biology,2019,17(8):e3000412.
[21] ZHENG Q T,LIN J,HUANG J,et al.Reconstitution of UCP1 using CRISPR/Cas9 in the white adipose tissue of pigs decreases fat deposition and improves thermogenic capacity[J].Proceedings of the National Academy of Sciences of the United States of America,2017,114(45):E9474-E9482.
[22] LIANG X J,PAN J F,CAO C W,et al.Transcriptional response of subcutaneous white adipose tissue to acute cold exposure in mice[J].International Journal of Molecular Sciences,2019,20(16):3968.

急性冷刺激后巴马猪背膘和腹股沟皮下脂肪组织的脂质组比较

Comparison of Lipid Profiles of Backfat and Inguinal Subcutaneous White Adipose Tissue from Acute Cold-treated Bama Pigs

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