›› 2015, Vol. 42 ›› Issue (8): 2124-2130.doi: 10.16431/j.cnki.1671-7236.2015.08.029

• 遗传繁育 • 上一篇    下一篇

Dppa5基因遗传多态性及其与母猪繁殖性状的关联分析

鲁慧文1,2, 郭若婷1, 刘宏杰2, 黄涛1   

  1. 1. 石河子大学动物科技学院, 石河子 832003;
    2. 新疆天康畜牧科技有限公司, 昌吉 831100
  • 收稿日期:2014-12-10 出版日期:2015-08-20 发布日期:2015-08-27
  • 通讯作者: 黄涛 E-mail:taohuang100@sina.com
  • 作者简介:鲁慧文(1987-),男,黑龙江绥化人,硕士生,研究方向:动物遗传育种与繁殖,E-mail:332903110@qq.com;郭若婷(1990-),女,新疆石河子人,硕士生,研究方向:动物遗传育种与繁殖,E-mail:674470976@qq.com
  • 基金资助:
    国家自然科学基金项目(30901014、31460586);国家国际科技合作项目(2014DFA31840)

Genetic Polymorphism of Dppa5 Gene and its Association with Reproductive Performance in Sow

LU Hui-wen1,2, GUO Ruo-ting1, LIU Hong-jie2, HUANG Tao1   

  1. 1. College of Animal Science and Technology, Shihezi University, Shihezi 832003, China;
    2. TECON Animal Husbandry Technology Corporation, Changji 831100, China
  • Received:2014-12-10 Online:2015-08-20 Published:2015-08-27

摘要: 试验旨在研究猪多潜能性维持因子5(developmental piuripotency associated 5,Dppa5)基因的遗传多态性及其与母猪繁殖性状的遗传效应。以长白猪和大白猪为试验群体,采用PCR-RFLP、克隆测序等方法分析检测猪Dppa5基因的多态性并对其与母猪产仔性能进行关联分析。结果表明,猪Dppa5基因外显子上存在2个错义突变位点:g.363 T> C和g.844 G> T;猪Dppa5基因g.363 T> C 位点对初产大白猪的总产仔数有极显著影响(P< 0.01),对经产大白猪、长白猪的产活仔数有显著影响(P< 0.05);g.844 G> T位点对经产长白猪和大白猪的总产仔数有显著影响(P< 0.05)。综合结果分析,Dppa5多态位点对猪的繁殖性能存在影响,可作为潜在的遗传标记,为猪的分子育种提供参考。

关键词: 猪; Dppa5基因; 繁殖性状

Abstract: The purpose of this study was to detect the genetic variation of the porcine developmental piuripotency associated 5 (Dppa5) gene and its association with reproductive performance in sow.PCR-RFLP and sequencing methods were used to detect SNPs and analyze its association with reproductive performance in Large White and Landrace sows.The results showed that two missense mutations g.363 T> C and g.844 G> T were detectd in exon of porcine Dppa5.SNP g.363 T> C affected total number born highly significantly in primiparous Large White sow (P< 0.01) and affected litter size and number born alive significantly in Large White and Landrace sows (P< 0.05).SNP g.844 G> T showed a significant impact on total number born in multiparous Landrace and Large White sows (P< 0.05).The results suggested that the SNPs of Dppa5 gene impacted reproductive performance of sows and could be genetic markers.

Key words: pig; Dppa5 gene; reproductive performance

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