关岭牛解偶联蛋白3基因5’侧翼区克隆和生物信息学分析

中国畜牧兽医 ›› 2014, Vol. 41 ›› Issue (9): 6-10.

关岭牛解偶联蛋白3基因5’侧翼区克隆和生物信息学分析

陈伟^1,2,3, 许厚强^1,2, 刘忠伟³, 陈祥^1,2, 张雯^1,2, 桓聪聪^1,2,3

1. 贵州大学动物科学学院, 贵州贵阳 550025;
2. 贵州大学, 高原山地动物遗传育种与繁殖省部共建教育部重点实验室, 贵州贵阳 550025;
3. 贵州大学生命科学学院, 贵州贵阳 550025

修回日期:2014-06-11 出版日期:2014-09-20 发布日期:2014-09-24
通讯作者: 许厚强 E-mail:houqiang0524@yahoo.com
作者简介:陈伟（1980- ），女，内蒙古人，博士生，研究方向：遗传学。
基金资助:
国家转基因生物新品种培育科技重大专项（2011ZX08009-004）；贵州省科技厅农业攻关计划（黔科NY字[2012]3008号）；贵州省科学技术基金项目（黔科合J字[2012]2145号）。

Cloning and Sequence Analysis of UCP3 Gene 5’-Flanking Region of Guanling Cattle

CHEN Wei^1,2,3, XU Hou-qiang^1,2, LIU Zhong-wei³, CHEN Xiang^1,2, ZHANG Wen^1,2, HUAN Cong-cong^1,2,3

1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Guizhou University, Guiyang 550025, China;
3. College of Life Science, Guizhou University, Guiyang 550025, China

Revised:2014-06-11 Online:2014-09-20 Published:2014-09-24

摘要/Abstract

摘要： 本试验对贵州关岭牛解偶联蛋白3（uncoupling protein，UCP3）基因5'侧翼区进行克隆，并利用生物信息学软件对其1080 bp的克隆序列进行分析，以研究UCP3基因5'侧翼区特异性转录调控元件的调控机制。软件分析发现关岭牛UCP3基因5'侧翼区含有6个可能的转录起始点和33个潜在转录因子结合位点，与东北虎、家猫、印度水牛、藏羚羊、山羊、绵羊UCP3基因5'侧翼区（-196～+100 bp）序列相似性分别为82%、82%、98%、95%、96%和98%；该区域保守性较强，推测转录起始点上游-200～+1 bp可能是其核心启动子区域，该区域在调控基因转录和翻译方面具有共同的作用。试验成功克隆了UCP3基因5'侧翼区序列1080 bp，初步预测了该基因的核心启动子区。

关键词: 关岭牛; 解偶联蛋白3基因; 启动子; 转录因子

Abstract: The experiment was conducted to clone 5'-flanking region of bovine UCP3 gene to study regulatory mechanism of the specific transcriptional regulation region. The 5'-flanking region of bovine UCP3 gene was amplified with PCR, and then the 1080 bp 5'-flanking promoter sequence was obtained by product purification, ligation, transformation, and sequence comparison. The obtained cloning vector of 5'-flanking region of bovine UCP3 gene was analyzed by the promoter software in this study. The results indicated that there were 6 transcription start sites and 33 potential transcription factor binding promoter region of the gene, the highly conserved region was in -196～+100 bp of the upstream of transcription start sites, which might be conclude that the -200～+1 bp of the upstream of transcription start sites was the core. Comparing bovine with Panthera tigris, Felis catus, Bubalus bubalis, Pantholops hodgsonii, Capra hircus, Ovis aries,the homology of 5'-flanking promoter sequence were 82%, 82%, 98%, 95%, 96% and 98%, respectively. The 1080 bp 5'-flanking region of the bovine UCP3 gene was cloned successfully and the core promoter region was preliminary predicted in the gene.

Key words: Guanling cattle; UCP3 gene; promoter; transcription factors

中图分类号:

Q785

陈伟, 许厚强, 刘忠伟, 陈祥, 张雯, 桓聪聪. 关岭牛解偶联蛋白3基因5’侧翼区克隆和生物信息学分析[J]. 中国畜牧兽医, 2014, 41(9): 6-10.

CHEN Wei, XU Hou-qiang, LIU Zhong-wei, CHEN Xiang, ZHANG Wen, HUAN Cong-cong. Cloning and Sequence Analysis of UCP3 Gene 5’-Flanking Region of Guanling Cattle[J]. , 2014, 41(9): 6-10.

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