使用粪球形态指标快速判别天山马鹿的性别

中国畜牧兽医 ›› 2014, Vol. 41 ›› Issue (9): 189-193.

使用粪球形态指标快速判别天山马鹿的性别

艾孜孜江·乃比, 夏米西丁·阿不都热依木, 布左拉·吐尔逊, 孜拉吉古丽·西克然木, 阿依努尔·阿卜杜艾尼, 古再努尔·孜比比拉, 热木图拉·阿卜杜克热木, 马合木提·哈力克

新疆大学生命科学与技术学院, 新疆乌鲁木齐 830046

收稿日期:2014-02-24 出版日期:2014-09-20 发布日期:2014-09-24
通讯作者: 马合木提·哈力克 E-mail:mahmuthalic@xju.edu.cn
作者简介:艾孜孜江·乃比（1986- ），男，新疆人，硕士生，研究方向：动物保护遗传学。
基金资助:
国家自然科学基金项目（31060152、31360266）；新疆维吾尔自治区自然科学基金项目（2010211A02）；新疆大学自治区重点学科——动物学科资助。

Study on Determining Sex of Cervus elaphus songaricus by Using of Pellet Morphometry

Ezizjan Nabi, Shamshidin Abduriyim, Buzohra Tursun, Zilajigvl Ekram, Aynur Abduhini, Guzalnur Zibibilla, Rahmutulla Abdukerim, Mahmut Halik

College of Life Sciences and Technology, Xinjiang University, Urumqi 830046, China

Received:2014-02-24 Online:2014-09-20 Published:2014-09-24

摘要/Abstract

摘要： 通过观察来自于新疆乌鲁木齐市南山山区的未知性别的天山马鹿种群粪便样本，依粪球形态可分为2类：子弹状、枣核状。子弹状呈短粗型，长宽比较小；枣核状呈细长型，长宽比较大。140份样品中子弹状86份、枣核状54份。通过扩增SRY基因进行分子鉴定知雄性94份、雌性46份。形态分类与实际性别吻合率88.34%，即子弹状为雄性，枣核状为雌性。并以样品长、宽平均值的比值（R）为指标快速聚类，建立了判别方程，统计指出判别结果与实际性别吻合率85.48%。结果提示今后的野外研究可直接利用粪球形态判定天山马鹿性别，长宽比判别方程可作为辅助。

关键词: 天山马鹿; 粪球形态; SRY基因; 性别鉴定

Abstract: By observing the unknown sex pellets collected from Cervus elaphus songaricus wild populations of Urumqi Nanshan mountain, all pellets are presented one of two shapes of bullet-like and jujube-stone like. The bullet-like fecal pellets displayed a short thick form with a low length/width ratio. The jujube-stone like ones displayed a thin long form with high length/width ratio. Using these shapes,the 140 samples were divided into two groups of 86 bullet-like and 54 jujube-stone like feces, and determined by PCR amplification of the SRY gene. Among of them,94 samples were identified as male,and the others as female. All the samples were clustered by the ratio of averaged length and width,and then the discrimination equation was established. The statistical results showed that the consistent degree of sex determination by fecal statistic index and DNA method was 85.48%,while morphological method and real sex was 88.34%. So,the bullet-like pellets were from male wapiti,and the jujube-stone like ones from females. The results showed that wapiti sex could be directly determined by its pellet shape in the wild. At the same time,the discrimination equation could be used as associated method.

Key words: Cervus elephus songaricus; pellet shape; SRY gene; sex determination

中图分类号:

S814.7

艾孜孜江·乃比, 夏米西丁·阿不都热依木, 布左拉·吐尔逊, 孜拉吉古丽·西克然木, 阿依努尔·阿卜杜艾尼, 古再努尔·孜比比拉, 热木图拉·阿卜杜克热木, 马合木提·哈力克. 使用粪球形态指标快速判别天山马鹿的性别[J]. 中国畜牧兽医, 2014, 41(9): 189-193.

Ezizjan Nabi, Shamshidin Abduriyim, Buzohra Tursun, Zilajigvl Ekram, Aynur Abduhini, Guzalnur Zibibilla, Rahmutulla Abdukerim, Mahmut Halik. Study on Determining Sex of Cervus elaphus songaricus by Using of Pellet Morphometry[J]. , 2014, 41(9): 189-193.

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