通过CHD基因快速鉴定鸽子性别方法的研究

doi:10.16431/j.cnki.1671-7236.2016.05.039

›› 2016, Vol. 43 ›› Issue (5): 1379-1384.doi: 10.16431/j.cnki.1671-7236.2016.05.039

通过CHD基因快速鉴定鸽子性别方法的研究

张莉, 单达聪, 刘彦, 潘裕华

北京市农林科学院畜牧兽医研究所, 畜禽疫病防控技术北京市重点实验室, 北京 100097

收稿日期:2015-12-11 出版日期:2016-05-20 发布日期:2016-05-23
通讯作者: 张莉 E-mail:Zhangli1620@sina.com
作者简介:张莉(1973-),女,陕西凤翔人,博士,副研究员,研究方向:畜禽分子生物学
基金资助:
北京市农委科技项目(2014-15)

Study on Quick Sex Identification Method in Pigeons by CHD Gene

ZHANG Li, SHAN Da-cong, LIU Yan, PAN Yu-hua

Beijing Key Laboratory for Prevention and Control of Infectious Diseases in Livestock and Poultry, Institute of Animal Husbandry and Veterinary Medicine, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China

Received:2015-12-11 Online:2016-05-20 Published:2016-05-23

摘要/Abstract

摘要： 本试验采用PCR方法,依据性别基因连锁理论,通过羽毛和血液,不提取鸽子基因组DNA,直接利用特异性引物对其CHD基因的特异性片段进行扩增,琼脂糖凝胶电泳检测,结果显示雄鸽仅有1条带,在700bp左右,雌鸽有2条带,分别在400与700bp左右;利用该检测方法对100羽性别未知的雏鸽进行了性别鉴定,结果为雌鸽48羽、雄鸽52羽,并通过剖解雄鸽的生殖器官进行验证,准确率达到100%。用建立的方法测定了2928羽雏鸽用于蛋鸽留种试验,结果表明,雌鸽为1411羽、雄鸽为1517羽。

关键词: 鸽子; 快速性别鉴定; CHD基因; PCR技术

Abstract: In this experiment, the primer pairs were designed, and polymerase chain reaction (PCR) technique to amplify the segments of the partial sex linked gene chromo-helicase-DNA-binding (CHD) to identificate pigeon's sex using feather pulps and blood.We found that two bands were demonstrated in all female egg-laying pigeons, and the sizes of them were 400 and 700 bp, respectively;In all male egg-laying pigeons, only one band appeared with length of 700 bp.100 unknown sex samples were successfully identified (52 males and 48 females).Based on the checking results between molecular electrophoresis of pigeons and the truly sex of maturity pigeons, we detected CHD gene in 2 928 early age pigeons using PCR amplification and electrophoresis in agarose gel.The PCR amplification results showed 1 517 males and 1 411 females.

Key words: pigeon; quick sex determination; CHD gene; PCR amplification

中图分类号:

S814.7

张莉, 单达聪, 刘彦, 潘裕华. 通过CHD基因快速鉴定鸽子性别方法的研究[J]. , 2016, 43(5): 1379-1384.

ZHANG Li, SHAN Da-cong, LIU Yan, PAN Yu-hua. Study on Quick Sex Identification Method in Pigeons by CHD Gene[J]. , 2016, 43(5): 1379-1384.

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通过CHD基因快速鉴定鸽子性别方法的研究

Study on Quick Sex Identification Method in Pigeons by CHD Gene

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