小鼠乳腺发育泌乳过程中L型氨基酸转运载体1的表达研究

中国畜牧兽医

小鼠乳腺发育泌乳过程中L型氨基酸转运载体1的表达研究

林叶，胡红柳，侯晓明

（东北农业大学乳品科学教育部重点实验室，黑龙江哈尔滨 150030）

收稿日期:2013-10-08 出版日期:2014-04-20 发布日期:2014-05-27
通讯作者: 侯晓明，副教授。E-mail：xiaominghou@126.com
作者简介:林叶（1980—），女，黑龙江人，博士，副教授，研究方向：泌乳生物学与乳腺功能调控。
基金资助:
2010年高等学校博士学科点专项科研基金联合资助（新教师类联合，20102325120018）；2012年黑龙江省政府博士后科研启动金（LBH-Q12157）；东北农业大学科技人才启动基金项目（校博士启动基金）。

Study on Expression of L-type Amino Acid Transporter 1 in Development and Lactation of Mouse Mammary Gland

LIN Ye, HU Hong-liu, HOU Xiao-ming

（Key Laboratory of Dairy Science of Ministry of Education, Northeast Agricultural University,Harbin 150030, China）

Received:2013-10-08 Online:2014-04-20 Published:2014-05-27

摘要/Abstract

摘要： 为阐明L型氨基酸转运载体1（L-type amino acid transporter 1，LAT1）的表达与乳腺发育和泌乳功能之间的关系，采用荧光定量RT-PCR技术和激光共聚焦显微技术对青春期、妊娠期、泌乳期和退化期小鼠乳腺中LAT1及其辅因子4F2抗原重链（4F2hc）表达含量和部位的变化进行研究。结果表明，青春期乳腺导管发育缓慢，LAT1和4F2hc在导管上皮细胞膜、肌上皮细胞膜及乳腺脂肪细胞膜上均低水平表达；妊娠期导管上皮细胞增殖分化加速，LAT1和4F2hc在乳腺小叶导管上皮细胞膜基底侧表达，表达水平上调；泌乳期乳蛋白合成和分泌旺盛，LAT1和4F2hc在腺泡上皮细胞膜的基底侧表达，表达量达到峰值；退化期乳腺组织功能性结构消退，乳腺对氨基酸的需求降低，LAT1和4F2hc的表达下降。提示，LAT1/4F2hc是小鼠乳腺组织中转运氨基酸的载体形式，LAT1和4F2hc的表达变化与乳腺发育、泌乳、退化的生理过程中氨基酸的需要量相关。

关键词: 乳腺发育; 氨基酸转运; LAT1; 4F2hc; 表达

Abstract: To investigate the underlying relationship between amino acid transporter expression and mammary gland development, the expression of LAT1 and 4F2hc in virgin, pregnancy, lactation and involution of mouse mammary gland was detected by RT-PCR and laser confocal microscope. The results showed that LAT1 and 4F2hc located on the membrane of ductal epithelial cell, myoepithelial cell and adipocyte in virgin mammary gland. The expression of LAT1 and 4F2hc was lower because duct development was slow in this period. In pregnancy, LAT1 and 4F2hc located on the basolateral of ductal epithelial cell. The expression of LAT1 and 4F2hc went up as ductal epithelial cell proliferation increased. In lactation, LAT1 and 4F2hc located on the basolateral of acinar epithelial cells. The expression of LAT1 and 4F2hc was the highest because of milk protein synthesis. In involution, acinar structure collapsed. The expression of LAT1 and 4F2hc decreased. All these results revealed that LAT1/4F2hc was an important amino acid transporter in mouse mammary gland. The expression of LAT1 and 4F2hc was related to amino acid requirement in mammary gland development and lactation.

Key words: mammary gland development; amino acid transportation; LAT1; 4F2hc; expression

林叶，胡红柳，侯晓明. 小鼠乳腺发育泌乳过程中L型氨基酸转运载体1的表达研究[J]. 中国畜牧兽医.

LIN Ye, HU Hong-liu, HOU Xiao-ming. Study on Expression of L-type Amino Acid Transporter 1 in Development and Lactation of Mouse Mammary Gland[J]. China Animal Husbandry & Veterinary Medicine.

参考文献

1 李健, 李庆章. 小鼠乳腺发育、泌乳和退化的组织形态学（Ⅰ）——一般组织形态学变化［J］. 东北农业大学学报, 2007, 38（2）: 196～201.
2 孟海洋, 孙晓旭, 王立娜, 等. 王不留行对奶牛乳蛋白合成信号转导通路的影响［J］. 中国畜牧兽医, 2013, 40（4）: 121～124.
3 Alemán G, López A, Ordaz G, et al. Changes in messenger RNA abundance of amino acid transporters in rat mammary gland during pregnancy, lactation, and weaning［J］. Metabolism, 2009, 58（5）: 594～601.
4 Anderson S M, Rudolph M C, McManaman J L, et al. Key stages in mammary gland development. Secretory activation in the mammary gland: It’s not just about milk protein synthesis［J］. Breast Cancer Res, 2007, 9（1）: 204～217.
5 Brer S, Wagner C A. Structure-function relationships of heterodimeric amino acid transporters［J］. Cell Biochem Biophys, 2002, 36（2～3）: 155～168.
6 Fotiadis D, Kanai Y, Palacín M. The SLC3 and SLC7 families of amino acid transporters［J］. Mol Aspects Med, 2013, 34（2～3）: 139～158.
7 Howlin J, McBryan J, Martin F. Pubertal mammary gland development: Insights from mouse models［J］. J Mammary Gland Biol Neoplasia, 2006, 11（3～4）: 283～297.
8 Ma X M, Blenis J. Molecular mechanisms of mTOR-mediated translational control［J］. Nat Rev Mol Cell Biol, 2009, 10（5）: 307～318.
9 Nicklin P, Bergman P, Zhang B, et al. Bidirectional transport of amino acids regulates mTOR and autophagy［J］. Cell, 2009, 136（3）: 521～534.
10 Proud C G. mTOR-mediated regulation of translation factors by amino acids［J］. Biochem Biophys Res Commun, 2004, 313（2）: 429～436.
11 Richert M M, Schwertfeger K L, Ryder J W, et al. An atlas of mouse mammary gland development［J］. J Mammary Gland Biol Neoplasia, 2000, 5（2）: 227～241.
12 Shennan D B, Calvert D T, Travers M T, et al. A study of L-leucine, L-phenylalanine and L-alanine transport in the perfused rat mammary gland: Possible involvement of LAT1 and LAT2［J］. Biochim Biophys Acta, 2002, 1564（1）: 133～139.

[1]	王小月, 苗家麟, 董旭晟, 王中华, 林雪彦, 王云, 侯秋玲. 动物乳腺干细胞研究进展[J]. 中国畜牧兽医, 2021, 48(2): 581-591.
[2]	吴玉莲, 叶升, 王国栋, 刘权辉, 黄奔. 山羊GATA3基因克隆及其真核表达载体构建[J]. 中国畜牧兽医, 2021, 48(1): 12-21.
[3]	贺越, 赵圣国, 骆超超, 郑楠, 王加启. 动物氨基酸转运载体的研究进展[J]. 中国畜牧兽医, 2020, 47(3): 744-753.
[4]	祁昊, 孟春雨, 高学军, 甄贞. SNAT2介导蛋氨酸对牛乳腺上皮细胞增殖与自噬的调节作用[J]. 中国畜牧兽医, 2019, 46(9): 2516-2525.
[5]	邵伟, 李杨, 王立文, 李翠, 木合依扎·热很别克, 余雄. 移植骨髓间充质干细胞对萨能奶山羊乳腺组织发育及血清激素水平的影响[J]. 《中国畜牧兽医》, 2017, 44(1): 74-79.
[6]	邢伟楠, 段晓宇, 杨洋, 侯晓明, 林叶. L型氨基酸转运载体1对奶牛乳腺中β-酪蛋白表达的影响[J]. 《中国畜牧兽医》, 2016, 43(10): 2553-2559.
[7]	谢雪娇, 栾生, 王媛, 张莉, 崔英俊, 王春梅, 李庆章. miR-142-3p对人乳腺上皮细胞MCF-10A增殖及乳蛋白合成的影响[J]. , 2015, 42(7): 1680-1685.
[8]	张抒, 张强, 张萌, 杨建成. miRNA对乳腺发育及泌乳调节作用的研究进展[J]. 《中国畜牧兽医》---唯一指定的官方网站, 2015, 42(3): 663-667.
[9]	林叶，侯晓明，田雷，齐帮若，李庆章. 必需氨基酸上调乳腺腺泡中L型氨基酸转运载体1的表达[J]. 中国畜牧兽医, 2014, 41(3): 83-86.
[10]	李丹, 王杰, 谢雪娇, 李庆章, 王春梅. miR-138对小鼠乳腺发育及泌乳功能的影响[J]. , 2014, 41(11): 223-227.