贵州本地山羊线粒体转录因子B2基因5′调控区SNP筛查与生物信息学分析

中国畜牧兽医

贵州本地山羊线粒体转录因子B2基因5′调控区SNP筛查与生物信息学分析

谢海强¹，刘若余¹，龚俞²，焦仁刚²

（1.贵州大学动物科学学院，高原山地动物遗传育种与繁殖教育部重点实验室，贵州省动物遗传育种与繁殖重点实验室，贵州贵阳 550025；2.贵州省畜牧技术推广站，贵州贵阳 550001）

收稿日期:2013-07-17 出版日期:2014-02-20 发布日期:2014-03-27
通讯作者: 刘若余（1963—），博士，教授，研究方向：分子遗传与动物育种。E-mail：liury04@163.com
作者简介:谢海强（1989—），男，江苏人，硕士生，研究方向：分子遗传与动物育种。

SNP Screening and Bioinformatics Analysis of 5′-flanking Region of TFB2M Gene in Local Goat in Guizhou Province

XIE Hai-qiang¹, LIU Ruo-yu¹, GONG Yu², JIAO Ren-gang²

(1. Guizhou Key Laboratory of Animal Genetics, Breeding and Reproduction, Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, College of Animal Science, Guizhou University， Guiyang 550025, China；2. Animal Science and Technology Station of Guizhou, Guiyang 550001, China)

Received:2013-07-17 Online:2014-02-20 Published:2014-03-27

摘要/Abstract

摘要： 试验旨在筛选线粒体转录因子B2（mitochondrial transcription factor B2, TFB2M）基因启动子区SNP，并研究其对启动子功能元件的影响。选择贵州本地优良品种黔北麻羊、贵州白山羊及贵州黑山羊3种山羊构建不同DNA池，直接测序筛选SNP位点。TFB2M基因5′调控区存在4个SNPs位点，分别为：G-601C、C-381A、C-286T和G-283T。生物信息学软件预测得到TFB2M基因核心启动子区和CpG岛，SNP位点导致附近大量转录因子新位点产生和原来结合位点消失，但并不改变CpG岛大小。TFB2M基因5′调控区存在4个对启动子功能元件有较大影响的SNPs位点。本研究结果为进一步确定TFB2M基因启动子功能奠定基础。

关键词: 线粒体转录因子B2基因; SNP; 启动子; 黔北麻羊; 贵州白山羊; 贵州黑山羊

Abstract: In order to screen the single nucleotide polymorphism （SNP） sites in the promoter region of mitochondrial transcription factor B2 （TFB2M） gene in goat and analyze the effect of SNP on function elements of promoter, three goat breeds (Qianbei Ma goat, Guizhou Black goat and Guizhou White goat) were selected to construct DNA pools, SNP sites were screened by direct sequencing subsequently. 4 SNPs which included G-601C, C-381A, C-286T and G-283T respectively were found in the 5′ flanking region of TFB2M gene. Furthermore, bioinformatics tools were used to predict the core region of the promoter and CpG island. It demonstrated that various novel transcription factors binding sites emerged while a large amount of transcription factors binding sites existed before disappeared based on the SNPs found in this study, while it didn’t affect the range of CpG island dramaticlly by using various softwares. There were four SNPs existed in the 5′ flanking region of TFB2M gene that had important effects on promoter elements. This work could lay the experimental foundation for the identification of function of TFB2M promoter.

Key words: TFB2M gene; SNP; promoter; Qianbei Ma goat; Guizhou White goat; Guizhou Black goat

谢海强，刘若余，龚俞，焦仁刚. 贵州本地山羊线粒体转录因子B2基因5′调控区SNP筛查与生物信息学分析[J]. 中国畜牧兽医.

XIE Hai-qiang, LIU Ruo-yu, GONG Yu, JIAO Ren-gang. SNP Screening and Bioinformatics Analysis of 5′-flanking Region of TFB2M Gene in Local Goat in Guizhou Province[J]. .

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