口蹄疫病毒Hankou/99株基因组全序列测定与基因特征分析

›› 2014, Vol. 41 ›› Issue (12): 74-80.

口蹄疫病毒Hankou/99株基因组全序列测定与基因特征分析

曾江勇

西藏农牧科学院畜牧兽医研究所, 西藏拉萨 850009

收稿日期:2014-07-30 出版日期:2014-12-20 发布日期:2014-12-25
作者简介:曾江勇(1973－), 男,湖南人,博士生,副研究员,研究方向:预防兽医与流行病学.
基金资助:
牦牛品种改良与高效养殖技术集成(2012BAD-03BO2).

Sequence Determination of Full Genome and Analysis of the Gene Characteristics of Hankou/99 Strain of Foot-and-mouth Disease Virus

ZENG Jiang-yong

Tibet Livestock Research Institute, Tibet Academy of Agricultural and Animal Sciences, Lhasa 850009, China

Received:2014-07-30 Online:2014-12-20 Published:2014-12-25

摘要/Abstract

摘要： 本研究主要对口蹄疫病毒(FMDV) Hankou/99株全基因序列进行测定,通过基因序列分析,确定其基因型,丰富了FMDV基因库,为研究猪源FMDV的分子变异、感染性分子克隆及致病机理奠定基础.从感染FMDV Hankou/99株的细胞液中提取RNA,通过RT-PCR技术,获得猪FMDV Hankou/99分离株覆盖全基因组的5个cDNA片段(S、L、C、D、E),分别对这些片段进行克隆和序列测定.结果显示,FMDV Hankou/99株全基因组长8099 bp;5'NCR长1040 bp,开放阅读框长6966 bp;3'NCR长93 bp,其后是30个碱基的连续poly(A)结构.通过与参考株基因组结构比较分析,显示其在分类地位上属于O型FMDV,并与猪源FMDV毒株OLZ、TW/97同源性较高,特别是3A区域上都有30 bp的缺失.另外,通过与9个参考株的VP1系统发生树分析,显示其与OLZ、TW/97、O/Akesu/58、O/OMⅢ 4个毒株为同一基因型.

关键词: 口蹄疫病毒; Hankou/99株; 全基因组; 序列测定; VP1

Abstract: Through sequencing and analyzing of full-length genome of Hankou/99 strain of foot-and-mouth disease virus (FMDV), the study identified type of the strain, which had enriched gene library of FMDV and provided a solid foundation for study of molecular variation, infectious molecular cloning and pathogenesis. Five gene fragments of S, L, C, D and E covering full-length genome of Hankou/99 isolate of FMDV extracted from infected cell fluid were obtained by RT-PCR technique, which were cloned and sequenced finally. The results indicated that full-length genome of the strain was 8099 bp including 5'NCR of 1040 bp ORF of 6966 bp and 3'NCR of 93 bp with 30 bp poly(A) at end of the genome. Through application of biological softwares, the strain could be ranked in O serotype FMDV. The strain had common characteristic of deletion of 30 bp in 3A region with the OLZ and TW/97 strains. In addition, the phylogenetic tree derived from the nucleotide sequence of VP1 gene from the strain and other nine reference strains showed that the strain presented higher homology with the four strains of OLZ, TW/97, O/Akesu/58, O/OMⅢ, which indicated that they belonged to the same genotype.

Key words: foot-and-mouth disease virus (FMDV); Hankou/99 strain; full-length genome; sequence determination; VP1

中图分类号:

S852.65

曾江勇. 口蹄疫病毒Hankou/99株基因组全序列测定与基因特征分析[J]. , 2014, 41(12): 74-80.

ZENG Jiang-yong. Sequence Determination of Full Genome and Analysis of the Gene Characteristics of Hankou/99 Strain of Foot-and-mouth Disease Virus[J]. , 2014, 41(12): 74-80.

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