β-catenin和BMP2基因在幼龄水貂皮肤中表达的研究

›› 2014, Vol. 41 ›› Issue (11): 14-19.

β-catenin和BMP2基因在幼龄水貂皮肤中表达的研究

王卓, 李光玉, 杨雅涵, 张铁涛, 张海华, 孙皓然, 孙伟丽

中国农业科学院特产研究所, 特种经济动物分子生物学国家重点实验室, 吉林长春 130112

修回日期:2014-07-20 出版日期:2014-11-20 发布日期:2014-12-06
通讯作者: 孙伟丽 E-mail:tcsswl@163.com
作者简介:王卓(1986-),女,吉林人,硕士生,研究方向:动物营养与饲料科学。
基金资助:
吉林省科技厅发展计划处青年科研基金(201201002);中国农业科学院科技创新工程经费。

Study on Expression of β-catenin and BMP2 Genes in Skin of Young Minks

WANG Zhuo, LI Guang-yu, YANG Ya-han, ZHANG Tie-tao, ZHANG Hai-hua, SUN Hao-ran, SUN Wei-li

State Key Laboratory of Special Economic Animal Molecular Biology, Institute of Special Animal and Plant Sciences, Chinese Academy of Agriculture Sciences, Changchun 130112, China

Revised:2014-07-20 Online:2014-11-20 Published:2014-12-06

摘要/Abstract

摘要： 本试验旨在探讨β-catenin和BMP2基因在幼龄水貂皮肤中定量表达的规律及意义。试验选用0～6周龄的幼龄公水貂21只,每周龄各3只,采集背中部的皮肤样品,运用实时荧光定量PCR技术相对定量方法, 检测不同周龄的幼龄水貂皮肤中β-catenin和BMP2基因相对表达量的变化。结果显示,随着周龄的增加β-catenin mRNA丰度呈上调趋势,在4周龄时显著增大(P<0.05),5周龄时达最大(P<0.01),6周龄时β-catenin相对表达量与5周龄相比极显著下降(P<0.01);而BMP2 mRNA的表达保持稳定(P>0.05)。β-catenin mRNA的丰度与次级毛囊发育变化趋势一致,与前人相关报道一致,提示其可能参与水貂次级毛囊发育;而BMP2 mRNA的丰度在毛囊发育阶段变化不明显。

关键词: 水貂; 幼龄; 皮肤; β-catenin; BMP2; 基因表达

Abstract: This experiment was designed to investigate the regularity and significance of β-catenin and BMP2 mRNA expression in skin of young minks. The mRNA expressions of β-catenin and BMP2 in the skin from 0 to 6 weeks old minks were studied by Real-time quantitative PCR relative quantitative method (ΔΔCt). The dorsocentral region of skin samples were collected from three minks per week. The results showed that β-catenin significantly up-regulated at 4 weeks old (P<0.05) and reached the maximum at 5 weeks old (P<0.01), then compared to 5 weeks old,it extremely significantly decreased at 6 weeks old (P<0.01), while the expression of BMP2 mRNA kept stable with weeks increasing (P>0.05). Combined the results of hair follicle histology of young mink, we could conclude that the abundance of β-catenin mRNA was correlated with secondary follicle development. It might be involved in the development of skin and secondary hair follicle in mink, and the abundance of BMP2 did not apparently change at different developmental stages of follicles.

Key words: mink; young; skin; β-catenin; BMP2; gene expression

中图分类号:

王卓, 李光玉, 杨雅涵, 张铁涛, 张海华, 孙皓然, 孙伟丽. β-catenin和BMP2基因在幼龄水貂皮肤中表达的研究[J]. , 2014, 41(11): 14-19.

WANG Zhuo, LI Guang-yu, YANG Ya-han, ZHANG Tie-tao, ZHANG Hai-hua, SUN Hao-ran, SUN Wei-li. Study on Expression of β-catenin and BMP2 Genes in Skin of Young Minks[J]. , 2014, 41(11): 14-19.

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