鸡主要组织相容性复合体Ⅰβ微球蛋白(β2m)基因的克隆及序列分析

›› 2014, Vol. 41 ›› Issue (10): 28-32.

鸡主要组织相容性复合体Ⅰβ微球蛋白(β2m)基因的克隆及序列分析

戴银, 张丹俊, 胡晓苗, 赵瑞宏, 侯宏艳, 潘孝成, 周学利, 沈学怀

安徽省农业科学院畜牧兽医研究所, 安徽合肥 230031

收稿日期:2014-03-25 出版日期:2014-10-20 发布日期:2014-10-29
通讯作者: 张丹俊
作者简介:戴银(1980－),女,安徽人,博士,助理研究员,研究方向:兽医微生物与免疫学。
基金资助:
国家自然科学基金项目(31302044);国家蛋鸡产业技术体系项目(CARS-41);安徽省现代农业肉禽产业发展资金项目和院科技创新团队(11C0404)

Cloning and Sequence Analysis of MHC Ⅰ β2m Gene in Chicken

DAI Yin, ZHANG Dan-jun, HU Xiao-miao, ZHAO Rui-hong, HOU Hong-yan, PAN Xiao-cheng, ZHOU Xue-li, SHEN Xue-huai

Institute of Animal Husbandry and Veterinary Medicine, Anhui Academy of Agricultural Sciences, Hefei 230031, China

Received:2014-03-25 Online:2014-10-20 Published:2014-10-29

摘要/Abstract

摘要： 为深入了解鸡主要组织相容性复合体Ⅰ(major histocompatibility complex class Ⅰ,MHCⅠ)β微球蛋白(β2m)分子的特征,研究β2m的免疫作用机理,试验通过RT-PCR技术获得了15条3个地方品种鸡完整的典型的MHCⅠβ2m基因,将该序列与GenBank数据库中登录的鸭、人、鼠、鱼、猴、马和猪7个不同物种的MHCⅠβ2m 分子序列进行了比对分析。结果显示,15条鸡β2m氨基酸序列同源性在98.0%~100%之间,大部分序列完全一致。鸡β2m氨基酸序列与鸭同源性最高,为60.6%;最低的则是来源于草鱼的β2m氨基酸序列,仅为33.3%。遗传进化分析进一步证实鸡与鸭的MHCⅠβ2m亲缘关系较近,与其他物种则遗传距离较远。此外,序列分析发现鸡β2m基因高度保守,仅个别碱基发生突变。其成熟肽序列的第24和79位为半胱氨酸,在所比对的动物中均未发生突变;同时在成熟肽的第77~83位之间存在免疫球蛋白超基因家族的特征基序“YXCXVXH”。研究结果表明,鸡与其他物种的MHCⅠβ2m分子具有相同的免疫功能基本单位,同时又具备独特的结构特点。

关键词: 鸡主要组织相容性复合体; β微球蛋白基因; 序列分析

Abstract: In order to further understand molecular characteristics and immune mechanisms of chicken major histocompatibility complex class Ⅰ(MHCⅠ) β2m gene,the 15 sequence of chicken MHC β2m gene from three local breeds was cloned by RT-PCR. We compared these sequences with seven MHCⅠβ2m genes of human,mouse and other animals in the GenBank database. The results showed that the amino acid homology among chicken MHCⅠβ2m ranged from 98.0% to 100%,the most sequences were identical. Chicken MHCⅠβ2m shared a 60.6% amino acid homology with duck,the highest degree of homology indicated a close genetic relationship,and the grasscarp had the lowest homology,33.3%. It could be further confirmed by phylogenetic analysis. Moreover,alignment of 10 β2m sequences of mature protein,two cysteines were located in sites 24 and 79,and there was the "YXCXVXH" Ig-motif character between the sites 77 and 83 of chicken β2m. The results showed that MHCⅠβ2m of chicken and other species had the same basic unit of immune function,and they had the unique structural features.

Key words: chicken MHC Ⅰ; β2m gene; sequence analysis

中图分类号:

戴银, 张丹俊, 胡晓苗, 赵瑞宏, 侯宏艳, 潘孝成, 周学利, 沈学怀. 鸡主要组织相容性复合体Ⅰβ微球蛋白(β2m)基因的克隆及序列分析[J]. , 2014, 41(10): 28-32.

DAI Yin, ZHANG Dan-jun, HU Xiao-miao, ZHAO Rui-hong, HOU Hong-yan, PAN Xiao-cheng, ZHOU Xue-li, SHEN Xue-huai. Cloning and Sequence Analysis of MHC Ⅰ β2m Gene in Chicken[J]. , 2014, 41(10): 28-32.

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