不同浓度胰岛素样生长因子-Ⅰ对体外培养奶牛乳腺上皮细胞乳蛋白和脂肪合成相关基因表达的影响

›› 2013, Vol. 40 ›› Issue (7): 1-5.

• 生物技术 • 下一篇

不同浓度胰岛素样生长因子-Ⅰ对体外培养奶牛乳腺上皮细胞乳蛋白和脂肪合成相关基因表达的影响

王皓宇, 秦彤, 郝海生, 杜卫华, 赵学明, 朱化彬

中国农业科学院北京畜牧兽医研究所, 北京 100193

收稿日期:2012-12-12 出版日期:2013-07-20 发布日期:2013-07-24
通讯作者: 秦彤,朱化彬 E-mail:qintong1980@yahoo.com.cn;zhuhuabin@iascaas.net.cn
作者简介:王皓宇(1988-),男,河北人,硕士生,研究方向:动物繁殖学。
基金资助:
国家"973"计划资助项目(2011CB100805);中央级科研院所基本科研业务费专项资金项目(2011cj-10-1)。

Effects of Different Concentration Insulin-like Growth Factor-Ⅰ on Expression of the Related Genes of Milk Protein and Fat Synthesis in Bovine Mammary Epithelial Cells Cultured in vitro

WANG Hao-yu, QIN Tong, HAO Hai-sheng, DU Wei-hua, ZHAO Xue-ming, ZHU Hua-bin

Institute of Animal Sciences, Chinese Academy of Agriculture Sciences, Beijing 100193, China

Received:2012-12-12 Online:2013-07-20 Published:2013-07-24

摘要/Abstract

摘要： 应用Real-time PCR方法检测不同浓度(0、1、10和100 ng/mL)胰岛素样生长因子-Ⅰ(IGF-Ⅰ)处理后的体外培养奶牛乳腺上皮细胞乳蛋白和脂肪合成相关基因mRNA的相对表达量。结果表明,添加不同浓度IGF-Ⅰ对体外培养的奶牛乳腺上皮细胞IGF-Ⅰ受体基因(IGFIR)、IGF-Ⅰ结合蛋白-3基因(IGFBP3)、α-s1-酪蛋白基因(CSN1S1)和κ-酪蛋白基因(CSN3)mRNA的相对表达丰度均无显著影响(P>0.05)。随着IGF-Ⅰ添加浓度的增加,β-酪蛋白基因(CSN2)、乙酰辅酶A羧化酶基因(ACACA)、脂肪酸合成酶基因(FASN)和脂肪酸结合蛋白-3基因(FABP3)mRNA的相对表达丰度显著上调(P<0.05)。提示,IGF-Ⅰ作为一种重要细胞因子参与调节乳腺上皮细胞乳蛋白和乳脂肪相关基因mRNA的表达。

关键词: 胰岛素样生长因子-Ⅰ; 乳腺上皮细胞; 实时荧光定量PCR; 基因表达

Abstract: The effects of different concentration (0, 1, 10 and 100 ng/mL) insulin-like growth factor-Ⅰ (IGF-Ⅰ) on the mRNA expression of genes related to milk protein and fat synthesis in bovine mammary epithelial cells cultured in vitro were evaluated by Real-time PCR. The results showed that the mRNA abundance of insulin like growth factor-Ⅰ receptor (IGFIR), insulin-like binding protein 3 (IGFBP3), α-s1-casein (CSN1S1) and κ-casein (CSN3) did not differ significantly (P>0.05) within different concentrations of IGF-Ⅰ. However, with the increasing concentrations of IGF-Ⅰ, the mRNA abundance of β-casein (CSN2), acetyl-CoA carboxylase (ACACA), fatty acid synthase (FASN) and fatty acid binding protein 3 (FABP3) increased significantly (P<0.05). These results indicated that IGF-Ⅰ, as an important cytokine, might involve in regulating the mRNA expression of genes related to milk protein and fat synthesis in bovine mammary epithelial cells cultured in vitro.

Key words: insulin like growth factor-Ⅰ; mammary epithelial cells; Real-time PCR; gene expression

中图分类号:

Q786

王皓宇, 秦彤, 郝海生, 杜卫华, 赵学明, 朱化彬. 不同浓度胰岛素样生长因子-Ⅰ对体外培养奶牛乳腺上皮细胞乳蛋白和脂肪合成相关基因表达的影响[J]. , 2013, 40(7): 1-5.

WANG Hao-yu, QIN Tong, HAO Hai-sheng, DU Wei-hua, ZHAO Xue-ming, ZHU Hua-bin. Effects of Different Concentration Insulin-like Growth Factor-Ⅰ on Expression of the Related Genes of Milk Protein and Fat Synthesis in Bovine Mammary Epithelial Cells Cultured in vitro[J]. , 2013, 40(7): 1-5.

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不同浓度胰岛素样生长因子-Ⅰ对体外培养奶牛乳腺上皮细胞乳蛋白和脂肪合成相关基因表达的影响

Effects of Different Concentration Insulin-like Growth Factor-Ⅰ on Expression of the Related Genes of Milk Protein and Fat Synthesis in Bovine Mammary Epithelial Cells Cultured in vitro

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