鲤鱼白细胞介素10基因组DNA克隆及序列分析

›› 2013, Vol. 40 ›› Issue (4): 65-68.

鲤鱼白细胞介素10基因组DNA克隆及序列分析

冯祥汝¹, 陈义龙¹, 孙真¹, 贾生美¹, 王文东², 张俊辉¹, 杨振国¹, 卢强¹

1. 吉林大学人兽共患病研究所,吉林长春 130062;
2. 吉林大学畜牧兽医学院,吉林长春 130062

收稿日期:2012-11-28 出版日期:2013-04-20 发布日期:2013-04-19
通讯作者: 卢强(1970-),男,硕士生导师,教授,从事人兽共患病毒病与分子免疫学研究。E-mail:qlu@jlu.edu.cn;Tel:0431-87836717 E-mail:qlu@jlu.edu.cn
作者简介:冯祥汝(1988-),女,辽宁人,硕士生,研究方向:兽医公共卫生学。
基金资助:
国家自然科学基金项目(30972277);吉林大学基本科研业务费项目(200903250)。

Cloning and Sequence Analysis of Interleukin 10 Genomic DNA in Common Carp (Cyprinus carpio L.)

FENG Xiang-ru¹, CHEN Yi-long¹, SUN Zhen¹, JIA Sheng-mei¹, WANG Wen-dong², ZHANG Jun-hui¹, YANG Zhen-guo¹, LU Qiang¹

1. Institute of Zoonoses, Jilin University, Changchun 130062, China;
2. College of Animal Science and Veterinary Medicine, Jilin University, Changchun 130062, China

Received:2012-11-28 Online:2013-04-20 Published:2013-04-19

摘要/Abstract

摘要： 为获得鲤鱼白细胞介素10(interleukin 10,IL-10)全长基因组序列,本研究利用鲤鱼IL-10全长cDNA序列(GenBank登录号:JX524550),通过在两端非编码区设计引物,以提取的鲤鱼脾脏基因组为模板,使用PCR方法成功获得鲤鱼IL-10全长基因组序列。鲤鱼IL-10基因组全长2176 bp,GenBank登录号为JX524551。将所获得的序列与其他物种IL-10基因组序列相比,结果发现都含有5个外显子,4个内含子,外显子在进化上相对保守,剪切位点都符合"gt......ag"规则;序列包含540 bp的开放阅读框,编码179个氨基酸,有2段典型的IL-10氨基酸信号基序。

关键词: 鲤鱼; 白细胞介素10; 基因组; 克隆; 序列分析

Abstract: The purpose of this study was to obtain the full-length sequence of common carp interleukin 10(IL-10) genomic DNA. The sequence of common carp IL-10 full-length cDNA(GenBank:JX 524550) was used. In this study, we designed two pairs of primers in its untranslated regions. Using the carp spleen genomic DNA as the template, we carried out PCR and obtained the IL-10 full-length genomic DNA sequence. The sequence was 2176 bp in length, and the GenBank accession number was JX524551. We compared the gained sequence with the other IL-10 and found their similarity of all harboring 5 exons and 4 introns. Moreover, all exons were comparatively conserved. The exons and introns splice sites were in accordance with "gt......ag" rules. Our results indicated that it was the sizes of introns that were crucial in the process of evolution.

Key words: common carp; interleukin 10; genome; cloning; sequence analysis

中图分类号:

Q785

冯祥汝, 陈义龙, 孙真, 贾生美, 王文东, 张俊辉, 杨振国, 卢强. 鲤鱼白细胞介素10基因组DNA克隆及序列分析[J]. , 2013, 40(4): 65-68.

FENG Xiang-ru, CHEN Yi-long, SUN Zhen, JIA Sheng-mei, WANG Wen-dong, ZHANG Jun-hui, YANG Zhen-guo, LU Qiang. Cloning and Sequence Analysis of Interleukin 10 Genomic DNA in Common Carp (Cyprinus carpio L.)[J]. , 2013, 40(4): 65-68.

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