›› 2013, Vol. 40 ›› Issue (4): 184-189.

• 遗传繁育 • 上一篇    下一篇

德宏水牛与尼里拉菲水牛及其杂交水牛的细胞遗传学研究

李思银1, 杨亮宇2, 杨玉艾3, 白文顺3   

  1. 1. 云南省昆明市石林县畜牧兽医总站,云南石林 652200;
    2. 云南农业大学外语学院,云南昆明 650201;
    3. 云南农业大学动物科学技术学院,云南昆明 650201
  • 收稿日期:2012-09-24 出版日期:2013-04-20 发布日期:2013-04-19
  • 通讯作者: 白文顺(1971-),女,硕士,研究方向:反刍动物营养。E-mail: 3078701767@163.com E-mail:3078701767@163.com
  • 作者简介:李思银(1987-),女,湖南人,硕士,研究方向:临床兽医学。
  • 基金资助:
    云南省科技厅项目(2009CD128)。

Cytogenetic Research on Dehong Buffalo,Nili-Ravi Buffalo and Their F1 Hybrid Bulls

LI Si-yin1, YANG Liang-yu2, YANG Yu-ai3, BAI Wen-shun3   

  1. 1. Animal Husbandry and Veterinary Shilin in Yunnan Province, Shilin 652200,China;
    2. College of Foreign Languages,Yunnan Agricultural University, Kunming 650201,China;
    3. College of Animal Science and Technology,Yunnan Agricultural University, Kunming 650201,China
  • Received:2012-09-24 Online:2013-04-20 Published:2013-04-19

摘要: 为了弄清河川型水牛与沼泽型水牛杂交后代的核型,以及与其育性和亲本核型的关系,用外周血液淋巴细胞培养法对尼里拉菲水牛、德宏水牛及尼里拉菲水牛×德宏水牛F1代公牛进行淋巴细胞培养,制作染色体标本,并进行G-带染色;利用BEION染色体核型分析系统对染色体标本分别进行染色体核型分析,确定其染色体核型。根据染色体核型分析结果,并结合其育性,分析染色体核型与其育性的关系。结果显示,尼里拉菲水牛核型为2n=50,XY;德宏水牛核型为2n=48,-4,-4,-9,-9,+t(4;9),+t(4;9),XY;尼里拉菲水牛×德宏水牛F1代公牛核型为:2n=49,-4,-9,+t(4;9),XY。

关键词: 德宏水牛; 尼里拉菲水牛; 尼里拉菲水牛×德宏水牛F1代公牛; 细胞遗传学

Abstract: In order to make clear the hybrid F1 (Dehong buffalo譔ili-Ravi buffalo) bulls karyotype and fertility and the relationship between them and the parents, the study cultrued lymphocyte in the hybrid F1 (Dehong buffalo, Nili-Ravi buffalo, Nili-Ravi譊ehong buffalo)males by peripheral blood lymphocyte culturing, made chromosomal sample and G-band dyeing. Then chromosome karyotypes in them were analysed and made sure by BEION Karyotyping system. The results showed that the karyotype of Dehong buffalo was 2n=50, XY, Nili-Ravi buffalo was 48,-4,-4,-9,-9,+t(4;9),+t(4;9),XY and hybrid F1(Nili-Ravi譊ehong buffalo) 49,-4,-9,+t(4;9),XY.

Key words: Dehong buffalo; Nili-Ravi buffalo; F1 hybrid bulls; cytogenetics

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