关键词: BPI基因; 编码区; 生物信息学

Abstract: The research was aimed to carry out the bioinformatics analysis on the coding region (CDS) of BPI gene among different species. By using the bioinformatics method, the genetic diversities of coding regions of BPI gene from the Homo sapiens, Mus musculus, Rattus norvegicus, Bos Taurus, Nomascus leucogenys, Gallus gallus, Oryctolagus cuniculus, Sus scrofa, Macaca mulatta, Equus caballus, Xenopus laevis, Pongo abelii and Canine were analyzed. The amino acid sequence, composition, signal peptide, hydrophobicity/hydrophilicity, trans-membrane structural domain, secondary structure and conservative structural domain of BPI protein were predicted and deduced. In 29 CDS sequences of BPI gene from 13 species, 532 polymorphic sites were detected and 15 haplotypes were generated. The coding region of BPI gene had the rich genetic diversity within and among species.BPI gene had strong codon bias.The theoretical isoelectric point of BPI protein was higher than 7. The protein was alkaline,and N terminal almost had the signal peptide. The peptide chain presented the hydrophilicity. There was a trans-membrane structural domain.The main secondary structure of BPI protein were α helix,β folding and random coil.There were two conservative structural domains named BPI1 and BPI2.

Key words: BPI gene; coding region; bioinformatics