4种佐剂对绵羊肺炎支原体的免疫辅佐效力研究

中国畜牧兽医 ›› 2013, Vol. 40 ›› Issue (3): 220-223.

4种佐剂对绵羊肺炎支原体的免疫辅佐效力研究

费磊¹, 岳华^1,2, 汤承^1,2

1. 西南民族大学生命科学与技术学院,四川成都 610041;
2. 动物医学四川省高等学校重点实验室,四川成都 610041

收稿日期:2012-11-14 出版日期:2013-03-20 发布日期:2013-03-19
通讯作者: 汤承(1965-),男,重庆人,硕士生导师,教授,研究方向:临床兽医学。E-mail:tangcheng101@yahoo.com.cn;Tel:028-85528276 E-mail:tangcheng101@yahoo.com.cn
作者简介:费磊(1987-),男,四川人,硕士生,研究方向:临床兽医学。
基金资助:
"十二五"国家科技支撑计划项目课题(2012BAD13B06)资助;四川省科技创新产业链示范工程项目—山羊现代产业链关键技术集成研究与产业化示范(2011NZ0003)。

Study on the Four Adjuvant for Inactivated Vaccine of Mycoplasma ovipneumoniae Immune Effectiveness

FEI Lei¹, YUE Hua^1,2, TANG Cheng^1,2

1. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China;
2. The Key Laboratory of Universities for Veterinary Medicine in Sichuan Province, Chengdu 610041, China

Received:2012-11-14 Online:2013-03-20 Published:2013-03-19

摘要/Abstract

摘要： 不同种类的佐剂对同种抗原的免疫辅佐效力不同。为筛选绵羊肺炎支原体(Mycoplasma ovipneumoniae,MO)灭活疫苗的理想佐剂,本研究制备了4种不同佐剂的MO灭活疫苗,分别免疫成年家兔,用MO间接血凝试剂盒测定免疫后1～9周血清抗体效价。检测结果显示,4种疫苗在免疫后都能迅速产生抗体,其中用MO Buonavoglial's和ISA-760佐剂疫苗免疫家兔,产生抗体均于免疫后第3周达到高峰((5.67±0.70) log2,6.00 log2),到第9周时维持抗体高峰(6.00 log2,(6.00±1.00) log2);MO ISA-206和白油佐剂疫苗免疫抗体均于免疫后第4周达到高峰((4.33±0.57) log2,5.00 log2),至第9周下降约1个滴度。统计学分析结果显示,ISA-760、Buonavoglial's佐剂对MO的免疫增强效力显著优于ISA-206和白油佐剂(P<0.05),是MO灭活疫苗的理想候选佐剂,为高效MO灭活疫苗的研发提供了科学依据。

关键词: 绵羊肺炎支原体; 灭活疫苗; 免疫效力; 佐剂

Abstract: Different types of adjuvants are different on the alloantigen immune adjuvant effect. To screen the ideal adjuvant of the inactivated vaccine of Mycoplasma ovipneumoniae (MO), the inactivated vaccines using 4 different adjuvants were respectively immunized adult rabbits and the determination of serum antibody titers were detected using the indirect hemagglutination kit after immunization from 1 to 9 weeks. The results showed that the 4 different vaccines could quickly produce antibodies after immunization. Furthermore, the rabbits antibodies using Buonavoglial's and ISA-760 adjuvant vaccines peaked at 3 weeks after vaccination ((5.67±0.70) log2,6.00 log2), and maintained the peak until in 9 weeks (6.00 log2,(6.00±1.00) log2). In addition to vaccines with MO ISA-206 and white oil adjuvant, the rabbit antibodies peaked in 4 weeks after immunization ((4.33±0.57) log2,5.00 log2) and decreased a titer until 9 weeks. According to the statistical analysis, the results showed that the immune enhancement effect of ISA-760 and Buonavoglial's adjuvant for the MO was significantly better than that of ISA-206 and white oil adjuvant (P<0.05). Therefore, the above results indicated that ISA-760 and Buonavoglial's were an ideal candidate for MO inactivated vaccine adjuvant and provided a scientific basis for studing the efficient of the inactivated vaccine for MO.

Key words: Mycoplasma ovipneumoniae; inactivated vaccine; immune effectiveness; adjuvant

中图分类号:

S858.28

费磊, 岳华, 汤承. 4种佐剂对绵羊肺炎支原体的免疫辅佐效力研究[J]. 中国畜牧兽医, 2013, 40(3): 220-223.

FEI Lei, YUE Hua, TANG Cheng. Study on the Four Adjuvant for Inactivated Vaccine of Mycoplasma ovipneumoniae Immune Effectiveness[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(3): 220-223.

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