免疫组化法检测促黄体素受体在多浪羊和卡拉库尔羊卵巢上的分布

›› 2013, Vol. 40 ›› Issue (3): 126-129.

免疫组化法检测促黄体素受体在多浪羊和卡拉库尔羊卵巢上的分布

王惠娥^1,2, 汪澜¹, 盛伟明¹

1. 塔里木大学动物科学学院,新疆塔里木 843300;
2. 新疆生产建设兵团塔里木畜牧科技重点实验室,新疆塔里木 843300

收稿日期:2012-08-29 出版日期:2013-03-20 发布日期:2013-03-19
作者简介:王惠娥(1977－),女,甘肃人,硕士生,讲师,研究方向:畜禽繁殖学。
基金资助:
兵团青年创新资金专项(2011CB001);校长基金(TDZKSS201106)。

Distribution and Localization of Luteinizing-hormone Receptors on the Ovaries between Duolang Sheep and Karakul Sheep

WANG Hui-e^1,2, WANG Lan¹, SHENG Wei-ming¹

1. College of Animal Science, Tarim University, Tarim 843300, China;
2. Key Laboratory of Tarim Animal Husbandry Science and Technology, Xinjiang Production and Construction Group, Tarim 843300, China

Received:2012-08-29 Online:2013-03-20 Published:2013-03-19

摘要/Abstract

摘要： 为从形态学角度理解内分泌调节过程,揭示促黄体生成素(luteotropic hormone,LH)对雌性哺乳动物卵巢调节作用机制,试验采用免疫组织化学SP方法研究促黄体生成素受体(luteotropic hormone receptors,LHR)在多浪羊和卡拉库尔羊卵巢组织中的分布与表达,分别选取相邻的5张连续切片,光镜观察、图像分析。结果显示,多浪羊与卡拉库尔羊的LHR阳性物质主要见于膜细胞、卵母细胞、颗粒细胞和血管周围间质,尤其在膜细胞和卵泡颗粒细胞的胞质中分布最多。原始卵泡卵母细胞中便有LHR受体阳性物质分布,在各级卵泡中LHR阳性细胞数量和染色强度随卵泡发育呈正向增加趋势。

关键词: 多浪羊; 卡拉库尔羊; 促黄体生成素; 促黄体生成素受体; 免疫组织化学SP染色法

Abstract: In order to understand the endocrine regulation process in a morphological view and reveal luteotropic hormone(LH) mechanism action in the female mammalian ovary, the distribution and localization of luteotropic hormone receptors(LHR) in ovaries of Duolang sheep and Karakul sheep were determined by immunohistochemical SP method. The results showed that 5 positive continuous sections were selected respectively. LHR-immunoreactive substance was mainly distributed over granulose cells, theca cells, oocyte, and ovary blood vessel surrounding interstitium. LHR was mainly loaded by the granulose cells and theca cells. Receptors regulation was showed during the earlier follicle period. With the development of follicles, the quantities and the staining intensity of the LHR-immunoreactive cells presented an increasing trend.

Key words: Duolang sheep; Karakul sheep; luteotropic hormone; luteotropic hormone receptors; immunohistochemical SP method

中图分类号:

Q579.1

王惠娥, 汪澜, 盛伟明. 免疫组化法检测促黄体素受体在多浪羊和卡拉库尔羊卵巢上的分布[J]. , 2013, 40(3): 126-129.

WANG Hui-e, WANG Lan, SHENG Wei-ming. Distribution and Localization of Luteinizing-hormone Receptors on the Ovaries between Duolang Sheep and Karakul Sheep[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(3): 126-129.

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