4个动物园灵长类动物肠道克雷伯氏菌的分离鉴定与药敏试验

›› 2013, Vol. 40 ›› Issue (2): 161-165.

4个动物园灵长类动物肠道克雷伯氏菌的分离鉴定与药敏试验

赵波^1,3, 李波², 王强^1,3, 余建秋^1,3, 杨光友², 陈红卫^1,3

1. 成都动物园,四川成都 610081;
2. 四川农业大学动物医学院,四川雅安 625014;
3. 成都野生动物研究所,四川成都 610081

收稿日期:2012-07-17 出版日期:2013-02-20 发布日期:2013-02-06
通讯作者: 杨光友(1964-),博士,教授,从事动物寄生虫病学研究。Tel:0835-2882787;E-mail:guangyou1963@yahoo.com.cn E-mail:guangyou1963@yahoo.com.cn
作者简介:赵波(1970-),男,四川人,高级兽医师,从事野生动物疾病防治研究。
基金资助:
成都大熊猫繁育研究基金项目(J08-4)。

Isolation,Identification and Antibiotics Sensitivity of Non-human Primates Klebsiella in Four Zoogical Gardens

ZHAO Bo^1,3, LI Bo², WANG Qiang^1,3, YU Jian-qiu^1,3, YANG Guang-you², CHEN Hong-wei^1,3

1. Chengdu Zoo, Chengdu 610081, China;
2. College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China;
3. Chengdu Wild Animal Institute, Chengdu 610081, China

Received:2012-07-17 Online:2013-02-20 Published:2013-02-06

摘要/Abstract

摘要： 从4个动物园的非人灵长类动物中采集了214份粪便样品,经过细菌分离培养、染色镜检和生化试验等分离鉴定出88株克雷伯氏菌,分离率为41.12%。按照美国临床实验室标准化委员会(CLSI)的判定标准,对分离到的88株克雷伯氏菌进行了22种抗生素药物的药敏试验,结果表明,88株均表现出不同的程度的耐药性,其中,耐药程度最高的是羧苄青霉素(耐药率为95.45%);敏感度最高的是阿米卡星(敏感率为77.27%)。88株克雷伯氏菌分离株均有较为严重的多重耐药性,耐8～15种抗菌药物的菌株最多,有64株,占分离菌株的72.73%;耐10种以上抗菌药物的菌株有63株,占分离菌株的71.59%。从不同动物园分离的克雷伯氏菌对22种抗生素的耐药情况来看,在所调查的4个动物园中,重庆某动物园(CQ)和北京某动物园(BJ)的克雷伯氏菌分离株间耐药率无差异(P>0.05);上海某野生动物园(SHW)和上海某动物园(SHZ)的克雷伯氏菌分离株间耐药率无差异(P>0.05);而北京某动物园(BJ)与上海某野生动物园(SHW)和上海某动物园(SHZ)的克雷伯氏菌分离株相比,其耐药率差异均极显著(P<0.01);重庆某动物园(CQ)与上海某野生动物园(SHW)和上海某动物园(SHZ)的克雷伯氏菌分离株相比,其耐药率差异均极显著(P<0.01)。

关键词: 动物园; 非人灵长类动物; 克雷伯氏菌; 生化鉴定; 药敏试验

Abstract: A total of 214 fecal samples were collected and examined for intestinal Klebsiella. 88 strains of Klebsiella were isolated and identified by a series of tests, including isolation of bacteria, biochemical tests, etc. The separation rate was 41.12%. According to the clinical and laboratory standards institute (CLSI), antimicrobial susceptibility test, against 22 kinds of common antibiotics and other antibiotics, was carried out for the 88 strains of Klebsiella. Our results indicated that the 88 strains of Klebsiella showed varying degrees of drug resistance. They were of the highest resistance to carbenicillin (drug resistance rate was 95.45%). They had the highest sensitivity to amikacin (sensitivity rate was 77.27%).The phenomenon of multiple drug resistance occurred in all strains of Klebsiella. 64 of them were indentified to be resistant to 8 to 15 kinds of antibiotics,63 of them were resistant to more than ten kinds of antibiotics. On the other hand, the differences of drug resistance rates of Klebsiella against 22 kinds of common antibiotics and other antibiotics in four zoological gardens were considered. There was no significant difference of drug resistance rates of Klebsiella collected in CQ and BJ(P>0.05). The same thing occurred in SHW and SHZ, the strains of Klebsiella collected from the two zoological gardens performed the similar drug resistance rates. However, the drug resistance rates of Klebsiella in both SHW and SHZ extremly significantly differed from either BJ or CQ(P<0.01).

Key words: zoogical garden; non-human primates; Klebsiella; biochemistry test; antimicrobial susceptibility test

中图分类号:

S852.61

赵波, 李波, 王强, 余建秋, 杨光友, 陈红卫. 4个动物园灵长类动物肠道克雷伯氏菌的分离鉴定与药敏试验[J]. , 2013, 40(2): 161-165.

ZHAO Bo, LI Bo, WANG Qiang, YU Jian-qiu, YANG Guang-you, CHEN Hong-wei. Isolation,Identification and Antibiotics Sensitivity of Non-human Primates Klebsiella in Four Zoogical Gardens[J]. , 2013, 40(2): 161-165.

参考文献

1 王海生. 抗感染药物合理应用[M].北京:人民卫生出版社,2002.
2 东秀珠,蔡妙英. 常见细菌系统鉴定手册[M]. 北京:科学出版社,2001.
3 孙长贵,成军. 2007年CLSI文件MI00-S17主要更新内容介绍[J]. 国际检验医学杂志,2007,28(12):1148～1150.
4 李淑红,王京仁,成钢,等. 猪大肠杆菌和葡萄球菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医,2012,39(8):195～198.
5 陈天寿. 微生物培养基的制造与应用[M]. 北京:中国农业出版社,1995.
6 陈永林,赵京,张成林,等. 长臂猿克雷伯氏菌病病原鉴定[J]. 中国兽药杂志,2007,41(10):58～59.
7 姚火春. 兽医微生物学试验指导[M]. 第2版. 北京:中国农业出版社,2002.
8 郭定宗,汪民权,周诗其,等. 金丝猴肺炎克雷伯氏菌及埃希氏大肠杆菌败血症的病理学观察[J]. 华中农业大学学报,2001,20(1):60～62.
9 高虹,张霞,关淳,等. 一种克雷伯氏菌分离培养基的研制[J]. 食品科学,2008,29(3):355～358.
10 国家标准化管理委员会.GB/T 14926.13—2001实验动物肺炎克雷伯杆菌检测方法[S].北京:中国标准出版社,2002.
11 Brisse S, Milatovic D, Fluit A C, et al. Epidemiology of quinolone resistance of Klebsiella pneumoniae and Klebsiella oxytoca in Europe[J]. Eur J Clin Microbiol Infect Dis,2000,19(1):64～68.
13 Escajadillo A. Laboratory maintenance and various experimental uses of the night monkey, Aotus[J]. Rev Med Panama,1984,9(3):274～289.
14 Fox J G, Rohovsky M W. Meningitis caused by Klebsiella spp in two rhesus monkeys[J]. J Am Vet Med Assoc,1975,167(7):634～636.
15 Holt J G. Bergey’s manual of determinative bacteriology[M]. Baltimor:Lippincott Williams & Wilkins Co.,1984.
16 Giles R J, Hildebrandt P K, Tate C. Klebsiella air sacculitis in the owl monkey (Aotus trivirgatus)[J]. Lab Anim Sci,1974,24(4):610～616.
17 Gozalo A, Montoyo E. Klebsiella pneumoniae infection in a New World nonhuman primate center[J]. Lab Primate Newsletter,1991,30:13～20.
18 Haeggman S, Lofdahl S, Paauw A, et al. Diversity and evolution of the class A chromosomal beta-lactamase gene in Klebsiella pneumoniae[J]. Antimicrob Agents Chemother,2004,48(7):2400～2408.
19 Janda J M, Abbott S L. The genera Klebsiella and Raoultella[M]. 3nd ed. ASM Press:Washington DC,2005.
20 Keynan Y, Rubinstein E. The changing face of Klebsiella pneumoniae infections in the community[J]. Int J Antimicrob Agents,2007,30(5):385～389.
21 Livermore D M. Beta-lactamases in laboratory and clinical resistance[J]. Clin Microbiol Rev,1995,8(4):557～584.
22 Paterson D L, Mulazimoglu L, Casellas J M, et al. Epidemiology of ciprofloxacin resistance and its relationship to extended-spectrum beta-lactamase production in Klebsiella pneumoniae isolates causing bacteremia[J]. Clin Infect Dis,2000,30(3):473～478.
23 Podschun R, Ullmann U. Klebsiella spp. as nosocomial pathogens: Epidemiology,taxonomy,typing methods,and pathogenicity factors[J]. Clin Microbiol Rev,1998,11(4):589～603.
24 Richard C. Epidemiology of Klebsiella pneumoniae infections in 2 colonies of squirrel monkeys and lemurs[J]. Bull Soc Pathol Exot Filiales,1989,82(4):458～464.
25 Snyder S B, Lund J E, Bone J, et al. A study of Klebsiella infections in owl monkeys (Aotus trivirgatus)[J]. J Am Vet Med Assoc,1970,157(11):1935～1939.
26 Stock I, Wiedemann B. Natural antibiotic susceptibility of Klebsiella pneumoniae, K.oxytoca, K.planticola, K.ornithinolytica and K.terrigena strains[J]. J Med Microbiol,2001,50(5):396～406.
27 Thom B T. Kiebsiella in faeces[J]. Lancet,1970,296:1033.
28 Van Kregten E, Westerdaal N A, Willers J M. New, simple medium for selective recovery of Klebsiella pneumoniae and Klebsiella oxytoca from human feces[J]. J Clin Microbiol,1984,20(5):936～941.

[1]	曹琪, 金清, 袁意, 冯逸雪, 孔丹妮, 陈翔鸿, 吴斌, 宋文博, 汤细彪. 规模化猪场常见呼吸道细菌性病原的流行病学调查[J]. 中国畜牧兽医, 2023, 50(6): 2439-2449.
[2]	卢玉葵, 刘佳琪, 钟嘉诚, 陈济铛, 朱婉君, 张溢珊, 张济培. 鹅源副鸡禽杆菌的分离鉴定及致病性研究[J]. 中国畜牧兽医, 2023, 50(6): 2562-2572.
[3]	罗干, 邹宏, 任绍科, 吴春霞, 邹瑶, 娄银莹, 周洋, 程方俊, 郭建华. 鸭源致病性大肠杆菌O抗原与毒力基因检测及药敏试验[J]. 中国畜牧兽医, 2023, 50(2): 713-721.
[4]	钟华晨, 王丽芳, 郭晨阳, 刘嘉琳, 宋洁. 内蒙古地区乳房炎奶样与环境中细菌的分离鉴定及耐药性分析[J]. 中国畜牧兽医, 2023, 50(2): 817-826.
[5]	杨福梅, 杨林富, 曾成, 杨志, 王康, 段纲, 代飞燕. 1株麂子源麦芽香肉杆菌的分离鉴定及生物学特性分析[J]. 中国畜牧兽医, 2023, 50(1): 398-407.
[6]	康宇晖, 周洪善, 金文杰, 秦爱建. 1株鹅源类志贺邻单胞菌的分离鉴定及致病性研究[J]. 中国畜牧兽医, 2022, 49(9): 3676-3682.
[7]	王治方, 徐引弟, 朱文豪, 张青娴, 焦文强, 李海利, 王克领. 副猪嗜血杆菌血清7型菌株毒力及耐药性分析[J]. 中国畜牧兽医, 2022, 49(8): 3245-3255.
[8]	李雨琪, 许丽婷, 阙群燕, 廖欣, 郑欣, 袁庆杨, 吴林悦, 谢欣杰, 田颖, 杨守深, 李晓华, 戴爱玲, 杨小燕, 范克伟. 豪猪源肠侵袭型大肠杆菌的分离鉴定及致病性研究[J]. 中国畜牧兽医, 2022, 49(11): 4372-4382.
[9]	葛强, 马东鑫, 周雨晴, 陈怀君, 袁敬知, 李珣, 王晓晔. 猪源奇异变形杆菌的分离鉴定及特征分析[J]. 中国畜牧兽医, 2021, 48(5): 1804-1815.
[10]	张天天, 吴征卓, 陈国权, 王娜, 周碧君, 程振涛, 文明, 王开功. 1株鸭源非O1/O139群霍乱弧菌的分离鉴定与致病性分析[J]. 中国畜牧兽医, 2021, 48(4): 1457-1465.
[11]	张备, 金振华, 孙金艳, 李烨, 王丽坤, 鹿凌岩, 史同瑞. 鹅大肠埃希菌的分离鉴定及系统进化树分析[J]. 中国畜牧兽医, 2021, 48(3): 1020-1026.
[12]	钟浪, 杨飞, 宋诗莹, 张振伟, 董海龙, 吴庆侠. 牦牛源溶血致病性表皮葡萄球菌的分离鉴定及药敏分析[J]. 中国畜牧兽医, 2021, 48(3): 1093-1101.
[13]	钟锐, 何廷美, 毕波, 杨兴涛, 施小刚, 金森龙, 冯刚, 彭广能. 浣熊源粪肠球菌的分离鉴定与耐药性分析[J]. 中国畜牧兽医, 2021, 48(3): 1112-1120.
[14]	邓智丹, 黄良宗, 白挨泉. 广东省1株猪2型链球菌的分离鉴定及部分生物学特性研究[J]. 中国畜牧兽医, 2021, 48(2): 717-725.
[15]	郭长明, 陈怀君, 朱善元, 袁敬知, 葛强, 李珣, 王晓晔. 广西某猪场猪链球菌分离鉴定及其生物学特性研究[J]. 中国畜牧兽医, 2021, 48(2): 736-746.