贵州荷斯坦奶牛甘油二酯酰基转移酶基因多态性及其与产奶性状的关联分析

›› 2013, Vol. 40 ›› Issue (12): 56-61.

贵州荷斯坦奶牛甘油二酯酰基转移酶基因多态性及其与产奶性状的关联分析

张驭纬¹, 谭志东¹, 付贤圣¹, 赵玲龙¹, 杨海兵¹, 杨胜林^1,2

1. 贵州大学动物科学学院, 贵州贵阳 550025;
2. 贵州大学高原山地动物遗传育种与繁殖省部共建教育部重点试验室, 贵州贵阳 550025

收稿日期:2013-05-09 出版日期:2013-12-20 发布日期:2014-02-11
通讯作者: 杨胜林 E-mail:shenglinyang@126.com
作者简介:张驭纬（1990- ），男，贵州人，学士，研究方向：动物遗传育种。
基金资助:
贵州省国际合作项目《与牛乳蛋白和乳脂性状相关的重要基因多态分析及其关联性研究》（黔科合外G字[2011]7005号）；贵州大学大学生SRT项目（贵大SRT字[2011]062号）。

Analysis of the Polymorphisms of DGAT1 Gene and its Association with Milk Production Traits in Guizhou Holstein Cows

ZHANG Yu-wei¹, TAN Zhi-dong¹, FU Xian-sheng¹, ZHAO Ling-long¹, YANG Hai-bing¹, YANG Sheng-lin^1,2

1. College of Animal Science, Guizhou University, Guiyang 550025, China;
2. Key Laboratory of Animal Genetics, Breeding and Reproduction in the Plateau Mountainous Region, Ministry of Education, Guizhou University, Guiyang 550025, China

Received:2013-05-09 Online:2013-12-20 Published:2014-02-11

摘要/Abstract

摘要： 甘油二酯酰基转移酶（diacylglycerol acyl-transferase 1，DGAT1）是控制甘油三脂合成的关键酶。近年来DGAT1基因被鉴定出来，被认为是奶牛乳脂率的一个重要功能候选基因。该研究以贵州荷斯坦奶牛为试验动物，利用奶牛DGAT1基因序列设计引物，以RCR-单链构象多态性（PCR-SSCP）方法检测了奶牛DGAT1基因第8外显子的碱基突变。结果共检测到AA、AB和BB 3种基因型，基因型频率分别为0.5588、0.3824和0.0588，等位基因A和B的频率分别为0.7549和0.2451；该碱基突变对305 d校正产奶量和乳脂率的影响均达到显著水平（P<0.05），乳蛋白率影响不显著（P>0.05）；多重比较结果表明，AA和AB型对305 d校正产奶量和乳脂率均显著高于BB型（P<0.05）。结果显示，DGAT1基因突变对贵州荷斯坦奶牛泌乳性状有较大的遗传效应，可用于其泌乳性状的分子标记辅助选择。

关键词: 贵州荷斯坦奶牛; 甘油二酯酰基转移酶基因; 产奶性能; 单链构象多态性

Abstract: Diacyglycerol acyl-transferase 1 (DGAT1) was considered to be the key enzyme in controlling the synthesis rate of triglycerides. The DGAT1 gene was recently identified,and DGAT1 gene became a strong functional candidate gene for milk fat percentage in cattle. This study used Guizhou Holstein cows as experimental animals,the cow DGAT1 gene was cloned by polymerase chain reaction using PCR primers. PCR-single-strand conformation polymorphism (PCR-SSCP)assay was used to diagnose the DGAT1 exon 8.The results showed that three genotypes were found and the genotypic frequencies of AA, AB and BB were 0.5588,0.3824 and 0.0588,respectively. The gene frequencies of A and B were 0.7549 and 0.2451.The significant association of the gene mutation in DGAT1 exon 8 with 305 days corrected milk yield and milk fat percentage (P<0.05), and had no significant effect on the protein percentage in milk (P>0.05). LSD showed that the cows with AA and AB genotypes had higher tested 305 days corrected milk yield and milk protein percentage than those with BB genotype (P<0.05).The conclusion indicated that the gene mutation in DGAT1 had a great genetic effect on milking traits and could be a useful genetic marker for Guizhou Holstein breeding.

Key words: Guizhou Holstein cows; DGAT1 gene; milk production traits; PCR-SSCP

中图分类号:

S823.9⁺1

张驭纬, 谭志东, 付贤圣, 赵玲龙, 杨海兵, 杨胜林. 贵州荷斯坦奶牛甘油二酯酰基转移酶基因多态性及其与产奶性状的关联分析[J]. , 2013, 40(12): 56-61.

ZHANG Yu-wei, TAN Zhi-dong, FU Xian-sheng, ZHAO Ling-long, YANG Hai-bing, YANG Sheng-lin. Analysis of the Polymorphisms of DGAT1 Gene and its Association with Milk Production Traits in Guizhou Holstein Cows[J]. China Animal Husbandry & Veterinary Medicine, 2013, 40(12): 56-61.

参考文献

1 毛永江,陈仁金,陈莹,等.南方地区中国荷斯坦牛DGAT1基因多态性与泌乳性状的关联分析[J].中国农业科学,2010,43(14):2990~2995.
2 王文君,欧阳克蕙,沈敏,等.影响奶牛产奶性状候选基因的研究进展[J].中国乳品工业,2008(5):49~51.
3 刘文艳,王长法,仲跻峰,等.二脂酰甘油酰基转移酶1基因的研究进展[J].中国畜牧兽医,2010,37(7):54~57.
4 张豪,张沅,张勤.后裔测定青年公牛的标记辅助BLUP选择[J].科学通报,2002,47(20):1566~1571.
5 陈慧勇,张勤,张沅,等.利用中国荷斯坦牛定位BTA6上影响产奶性状的QTLs[J].科学通报,2005,50(15):1594~1599.
6 贾晋,马妍,孙东晓,等.中国荷斯坦牛DGAT1基因与产奶性状关联分析[J].畜牧兽医学报,2008,39(12):1661~1664.
7 Cases S,Smith S J,Zheng Y W,et al. Identification of a gene encoding an acyl CoA:Diacylglycerol acyltransferase,a key enzyme in triacylglycerol synthesis.Proceeding of the national academy of sciences of the United States of America.1998.
8 Conte G,Mele M,Chessa S,et al. Diacylglycerol acyltransferase 1,stearoyl-CoA desaturase 1 and sterol regulatory element binding protein 1 gene polymorphisms and milk fatty acid composition in Italian brown cattle[J].Journal of Dairy Science,2010,93:753~763.
9 Demeter R M,Schopen G C B,Oude Lansink A G J M,et al.Effects of milk fat composition,DGAT1,and SCD1 on fertility traits in Dutch Holstein cattle[J].Journal of Dairy Science,2009,92:5720~5729.
10 Falconer D S,Mackay T F C. Introduciton to quantitiative genetics[M].4th end. Essex:Longman,1996.
11 Jureeratn S,Sayan B,Kanokporn T. Effect of DGAT1 major gene on milk production and quality traits in Thai-Holstein dairy crossbred[J].Journal of Biotechnology for Livestock Production,2010,5(1):9~20.
12 Kaupe B,Winter A,Fries R,et al.DGAT1 polymorphism in Bos indicus and Bos taurus cattle breeds[J].Journal of Dairy Research,2004,71:182~187.
13 Naslund J,Fikse W F,Pielberg G R,et al. Frequency and effect of the bovine Acyl-CoA:Diacylglycerol acyltransferase 1(DGAT1) K232A polymorphism in Swedish dairy cattle[J].Journal of Dairy Science,2008,91:2127~2134.
14 Nishizuka Y. Intracellular signaling by hydrolysis of phospholipids and activation of protein kinase C[J].Science,1992,258(5082):607~614.
15 Ogorevc J,Kunej T,Razpet A,et al. Database of cattle candidate genes and genetic markers for milk production and mastitis[J].Animal Genetics,2009,40:832~851.
16 Oikonomou G,Angelopoulou K,Arsenos G,et al. The effects of polymorphisms in the DGAT1,leptin and growth hormone receptor gene loci on body energy,blood metabolic and reproductive traits of Holstein cows[J].Animal Genetics,2008,40:10~17.
17 Pannier L,Mullen A M,Hamill R M,et al.Association analysis of single nucleotide polymorphisms in DGAT1,TG and FABP4 genes and intramuscular fat in crossbred Bos taurus cattle[J].Meat Science,2010,85(3):515~518.
18 Pareek C S,Czarnik U,Zabolewicz T,et al.DGAT1 K232A quantitative trait nucleotide polymorphism in Polish Black-and-White cattle[J].Journal of Applied Genetics,2005,46:85~87.
19 Schennink A,Heck J M L,Bovenhuis H,et al. Milk fatty acid unsaturation:genetic parameters and effects of stearoyl-CoA desaturase(SCD1)and acyl CoA:Diacylglycerol acyltransferase 1(DGAT1)[J].Journal of Dairy Science,2008,91:2135~2143.
20 Schennink A,Stoop W M,Visker M H P W,et al. DGAT1 unterlies large genetic variation in milk fat composition of dairy cows[J].Animal Genetics,2007,38:467~473.
21 Signorelli F,OrruL N F,Matteis G D,et al. Exploring polymorphisms and effects oil milk traits of the DGAT1,SCD1 and GHR genes in four cattle breeds[J].Livestock Science,2009,125:74~79.
22 Spelman R J,Ford C A,McElhinney P,et al. Characterization of the DGAT1 gene in the New Zealand dairy population[J].Journal of Dairy Science,2002,85:3514~3517.
23 Sun D,Jia J,Ma Y,et al. Effect of DGAT1 and GHR on milk yield and milk composition in the Chinese dairy population[J].Animal Genetics,2009,40:997~1000.
24 Thaller G,Kramer W,Winter A,et al. Effects of DGAT1 variants on milk production traits in German cattle breeds[J].Journal of Animal Science,2003,81(8):1911~1918.
25 Winter A,van Eckeveld M,Bininda-Emonds O R,et al. Genomic organization of the DGAT2/MOGAT gene family in cattle(Bos taurus)and other mammals[J].Cytogenetic and Genome Research,2003,102(1~4):42~47.

[1]	宁景扬, 马龙刚, 陈紫薇, 安涛, 常瑶, 王凯, 王磊, 陈少侃, 张帆, 郭刚, 张海亮, 王雅春. 中国荷斯坦牛干奶期长度的群体特征及其与生产性能的关系[J]. 中国畜牧兽医, 2021, 48(9): 3368-3377.
[2]	王亚男, 冯曼, 周英昊, 毛森, 邱殿锐, 郭建军, 李素霞, 王洪彬. 冀北寒区秋冬季节荷斯坦奶牛产奶性能及血液常规指标的变化规律研究[J]. 中国畜牧兽医, 2020, 47(4): 1282-1288.
[3]	马大川, 武杰, 李娟, 单春乔. 复合酶与复合酶菌制剂对日粮体外降解率及不同泌乳阶段奶牛产奶性能的影响[J]. 中国畜牧兽医, 2020, 47(12): 3917-3925.
[4]	文惠, 罗汉鹏, 米思远, 刘雪琴, 王雅春, 肖炜, 俞英. 副乳头对奶牛生产性能的影响及其相关基因LGR5的遗传表达分析[J]. 中国畜牧兽医, 2019, 46(12): 3642-3649.
[5]	杨永强, 龚俞, 焦仁刚, 惠嫣婷, 刘若余. 牛α-乳清蛋白基因5'调控区多态性研究[J]. , 2013, 40(1): 136-140.
[6]	崔莲花, 严昌国, Kim Hyen, 辛宗瑞. 发芽玉米对荷斯坦奶牛瘤胃发酵、产奶性能及乳品质的影响[J]. , 2012, 39(11): 94-99.
[7]	王建平;王加启;卜登攀;霍小凯;郭同军;梁建光;王光文;袁耀明;李发弟 . 上海地区季节变化对奶牛产奶性能影响的研究[J]. , 2008, 1(8): 70-73.
[8]	陈松明. 采用物理降温措施对奶牛夏季产奶性能影响的研究[J]. , 2007, 34(9): 92-94.
[9]	宋文学;谢光洪;王哲. 分子生物学技术在临床兽医学上的应用[J]. , 2007, 34(6): 93-95.
[10]	张石蕊;易学武;刘海林;刘雁英;谭新. 橘皮渣对奶牛产奶性能和养分表观消化率的影响[J]. , 2007, 34(6): 61-63.