QseBC双组份调控系统研究进展

摘要/Abstract

摘要： QseBC双组份调控系统是一种广泛存在于革兰氏阴性菌内部的双组份调控系统,在调控细菌毒力方面发挥重要作用。作者综述了QseBC双组份调控系统的组成及其在细菌致病性中的研究进展,重点介绍了该调控系统在大肠杆菌、沙门氏菌、放线杆菌、爱德华氏菌和嗜水气单胞菌致病性中的作用。对QseBC双组份调控系统的深入研究,有助于开发以细菌毒力为靶点的新一代抗菌药物,为解决细菌耐药问题提供新思路。

关键词: QseBC; 双组份调控系统; 致病性

Abstract: The QseBC two-component regulatory system is widespread in the gram-negative bacillus, which plays an important role in the regulation of bacterial virulence. In this article, the composition of the QseBC two-component regulatory system and its role in bacterial pathogenicity, especially in E.coli, S.typhimurium, Actinobacillus, E.tarda and A.hydrophila were summarized. The further study on QseBC may aid to drug design based on the bacterial virulence as the new targets, and provide new ideas for resolving the problem of antimicrobial resistance.

Key words: QseBC; two-component regulatory system; pathogenicity

元雪浈, 杜向党, 秦上尚, 李新生. QseBC双组份调控系统研究进展[J]. , 2013, 40(11): 204-208.

YUAN Xue-zhen, DU Xiang-dang, QIN Shang-shang, LI Xin-sheng. Research Progress on the QseBC Two-component Regulatory System[J]. , 2013, 40(11): 204-208.

参考文献

1 冯向辉,徐莉,孟焕新,等. 慢性牙周炎患者抗伴放线放线杆菌IgG滴度改变的临床意义[J].北京大学学报,2010,42(2):207~210.
2 刘春花,张彦明,张耀相. 胸膜肺炎放线杆菌研究进展[J].中国兽医学报,2003,23(2):206~208.
3 张翠娟,于宙亮,赵宝华,等. 嗜水气单胞菌研究进展[J].中国兽药杂志,2008,42(7):46~50.
4 贺扬,张晓华. 迟缓爱德华氏菌致病相关因子的研究进展[J].中国海洋大学学报,2009,39(5):979~987.
5 顾欣,金凌艳,蔡金华,等. 国家动物源细菌耐药性监测工作的探讨和建议[J].中国兽药杂志,2009,43(7):45~50.
6 Alban A,David S O,Bjorkesten L,et al. A novel experimental design for comparative two-dimensional gel analysis: Two-dimensional difference gel electrophoresis incorporating a pooled internal standard[J]. Proteomics,2003,3(1):36~44.
7 Bearson B L, Bearson S M. The role of the QseC quorum-sensing sensor kinase in colonization and norepinephrine-enhanced motility of Salmonella enterica serovar typhimurium[J]. Microb Pathog,2008,44(4):271~278.
8 Bearson B L, Bearson S M, Lee I S, et al. The Salmonella enterica serovar typhimurium QseB response regulator negatively regulates bacterial motility and swine colonization in the absence of the QseC sensor kinase[J]. Microb Pathog,2010,48(6):214~219.
9 Brzuszkiewicz E, Bruggemann H, Liesegang H, et al. How to become a uropathogen: Comparative genomic analysis of extraintestinal pathogenic Escherichia coli strains[J]. Proc Natl Acad Sci USA,2006,103(34):12879~12884.
10 Clarke M B, Sperandio V. Transcriptional autoregulation by quorum sensing Escherichia coli regulators B and C (QseBC) in enterohaemorrhagic E.coli (EHEC)[J]. Mol Microbiol,2005a,58(2):441~455.
11 Clarke M B, Sperandio V. Transcriptional regulation of flhDC by QseBC and sigma (FliA) in enterohaemorrhagic Escherichia coli[J]. Mol Microbiol,2005b,57(6):1734~1749.
12 Clarke M B, Hughes D T, Zhu C, et al. The QseC sensor kinase: A bacterial adrenergic receptor[J]. Proc Natl Acad Sci USA,2006,103(27):10420~10425.
13 Darling A E, Mau B, Perna N T. Progressive mauve: Multiple genome alignment with gene gain, loss and rearrangement[J]. PLoS One,2010,5(6):e11147.
14 Durham-Colleran M W, Verhoeven A B, van Hoek M L. Francisella novicida forms in vitro biofilms mediated by an orphan response regulator[J]. Microb Ecol,2010,59(3):457~465.
15 Garofalo C K, Hooton T M, Martin S M, et al. Escherichia coli from urine of female patients with urinary tract infections is competent for intracellular bacterial community formation[J]. Infect Immun,2007,75(1):52~60.
16 Hadjifrangiskou M, Kostakioti M, Chen S L, et al. A central metabolic circuit controlled by QseC in pathogenic Escherichia coli[J]. Mol Microbiol,2011,80(6):1516~1529.
17 Hughes D T, Clarke M B, Yamamoto K, et al. The QseC adrenergic signaling cascade in enterohemorrhagic E.coli (EHEC)[J]. PLoS Pathog,2009,5(8):e1000553.
18 Justice S S, Hung C, Theriot J A, et al. Differentiation and developmental pathways of uropathogenic Escherichia coli in urinary tract pathogenesis[J]. Proc Natl Acad Sci USA,2004,101(5):1333~1338.
19 Khajanchi B K, Kozlova E V, Sha J, et al. The two-component QseBC signalling system regulates in vitro and in vivo virulence of Aeromonas hydrophila[J]. Microbiology,2012,158(Pt1):259~271.
20 Kostakioti M, Hadjifrangiskou M, Pinkner J S, et al. QseC-mediated dephosphorylation of QseB is required for expression of genes associated with virulence in uropathogenic Escherichia coli[J]. Mol Microbiol,2009,73(6):1020~1031.
21 Li L, Xu Z, Zhou Y, et al. Global effects of catecholamines on Actinobacillus pleuropneumoniae gene expression[J]. PLoS One,2012,7(2):e31121.
22 Merighi M, Septer A N, Carroll-Portillo A, et al. Genome-wide analysis of the PreA/PreB (QseB/QseC) regulon of Salmonella enterica serovar typhimurium[J]. BMC Microbiol,2009,9:42.
23 Moreira C G, Weinshenker D, Sperandio V. QseC mediates Salmonella enterica serovar typhimurium virulence in vitro and in vivo[J]. Infect Immun,2010,78(3):914~926.
24 Novak E A, Shao H, Daep C A, et al. Autoinducer-2 and QseC control biofilm formation and in vivo virulence of Aggregatibacter actinomycetemcomitans[J]. Infect Immun,2010,78(7):2919~2926.
25 Parker C T, Sperandio V. Cell-to-cell signalling during pathogenesis[J]. Cell Microbiol,2009,11(3):363~369.
26Rasko D A, Moreira C G, Li D R, et al.Targeting QseC signaling and virulence for antibiotic development[J]. Science,2008,321(5892):1078~1080.
27 Reading N C, Torres A G, Kendall M M, et al. A novel two-component signaling system that activates transcription of an enterohemorrhagic Escherichia coli effector involved in remodeling of host actin[J]. J Bacteriol,2007,189(6):2468~2476.
28 Rosen D A, Hooton T M, Stamm W E, et al. Detection of intracellular bacterial communities in human urinary tract infection[J]. PLoS Med,2007,4(12):e329.
29 Sperandio V, Torres A G, Kaper J B. Quorum sensing Escherichia coli regulators B and C (QseBC): A novel two-component regulatory system involved in the regulation of flagella and motility by quorum sensing in E.coli[J]. Mol Microbiol,2002,43(3):809~821.
30 Sperandio V, Torres A G, Jarvis B, et al. Bacteria-host communication: The language of hormones[J]. Proc Natl Acad Sci USA, 2003, 100(15):8951~8956.
31 Unal C M, Singh B, Fleury C, et al. QseC controls biofilm formation of non-typeable Haemophilus influenzae in addition to an AI-2-dependent mechanism[J]. Int J Med Microbiol,2012,302(6):261~269.
32 [KG*2][ZK(#]Wang Q, Yang M, Xiao J, et al. Genome sequence of the versatile fish pathogen Edwardsiella tarda provides insights into its adaptation to broad host ranges and intracellular niches[J]. PLoS One,2009,4(10):e7646.
33 Wang X, Wang Q, Yang M, et al. QseBC controls flagellar motility, fimbrial hemagglutination and intracellular virulence in fish pathogen Edwardsiella tarda[J]. Fish Shellfish Immunol,2011,30(3):944~953.
34 Wright K J, Seed P C, Hultgren S J. Development of intracellular bacterial communities of uropathogenic Escherichia coli depends on type 1 pili[J]. Cell Microbiol,2007,9(9):2230~2241.

[1]	程前, 高清清, 王雨禾, 郇长超, 高崧. 禽致病性大肠杆菌外膜蛋白OmpA对DF-1细胞自噬的影响[J]. 中国畜牧兽医, 2023, 50(5): 1971-1980.
[2]	王路才, 撒朗文朱, 张焕容. 禽源肠炎沙门菌的分离鉴定及耐药性与致病性分析[J]. 中国畜牧兽医, 2023, 50(4): 1663-1674.
[3]	康宇晖, 周洪善, 金文杰, 秦爱建. 1株鹅源类志贺邻单胞菌的分离鉴定及致病性研究[J]. 中国畜牧兽医, 2022, 49(9): 3676-3682.
[4]	邵明珠, 赵允清, 李松励, 侯绍华, 张艳英. 河北省奶牛源大肠杆菌的分离鉴定、致病性及中草药体外抑菌效果研究[J]. 中国畜牧兽医, 2022, 49(9): 3683-3691.
[5]	王治方, 徐引弟, 朱文豪, 张青娴, 焦文强, 李海利, 王克领. 副猪嗜血杆菌血清7型菌株毒力及耐药性分析[J]. 中国畜牧兽医, 2022, 49(8): 3245-3255.
[6]	郭骞, 张钰, 方小伟, 安妮, 袁梅, 方春. 糖基翻转酶gtcA基因缺失降低单增李斯特菌1/2a血清型菌株致病性的研究[J]. 中国畜牧兽医, 2022, 49(6): 2298-2306.
[7]	王思敏, 杨江峰, 赵晓锟, 张雷, 郭晶晶, 兰金苹, 于小杰, 王净. 羊源沙门氏菌的分离鉴定及致病性和耐药性分析[J]. 中国畜牧兽医, 2022, 49(6): 2385-2396.
[8]	董洪燕, 宋海港, 朱善元, 于圣青, 谢军, 洪双鹏, 郭长明, 徐海, 高月秀. 鸭源大肠杆菌的分离鉴定及致病性分析[J]. 中国畜牧兽医, 2022, 49(5): 1977-1984.
[9]	侯晓红, 王蒙, 武博, 龙宪荣, 赵孝民, 高静, 戴培强, 柴同杰. H9N2亚型禽流感病毒SD18株的传染性及不同途径感染效果比较[J]. 中国畜牧兽医, 2022, 49(4): 1449-1460.
[10]	兰虹, 任广彩, 叶俊贤, 熊挺, 何献铭, 刘郁夫, 杨泽坤, 郝文茜, 陈瑞爱. Ⅰ群禽腺病毒血清11型的分离鉴定及致病性试验[J]. 中国畜牧兽医, 2022, 49(3): 1067-1076.
[11]	陈小飞, 张斌, 张春红, 张建峰, 廖明. 猪A群轮状病毒SCJY-13株的分离鉴定及致病性分析[J]. 中国畜牧兽医, 2022, 49(2): 660-668.
[12]	张豪磊, 撒朗文朱, 黄志宏, 文永平, 张焕容. 鸭源沙门菌的分离鉴定、耐药性及致病性分析[J]. 中国畜牧兽医, 2022, 49(12): 4887-4897.
[13]	李雨琪, 许丽婷, 阙群燕, 廖欣, 郑欣, 袁庆杨, 吴林悦, 谢欣杰, 田颖, 杨守深, 李晓华, 戴爱玲, 杨小燕, 范克伟. 豪猪源肠侵袭型大肠杆菌的分离鉴定及致病性研究[J]. 中国畜牧兽医, 2022, 49(11): 4372-4382.
[14]	薛晓岩, 张振兴, 曾毅, 季佳, 代红炀, 李素华, 宋建领. 禽腺病毒4型云南株分离鉴定及Hexon基因序列分析[J]. 中国畜牧兽医, 2022, 49(10): 3932-3942.
[15]	张甜甜, 吕文亮, 林显华, 鹿瑶, 徐海燕, 谷巍, 郝木强, 盛永杰. 1株鸡源肠炎沙门氏菌的分离鉴定及致病性分析[J]. 中国畜牧兽医, 2022, 49(10): 4097-4105.