›› 2012, Vol. 39 ›› Issue (3): 171-173.

• 疾病防治 • 上一篇    下一篇

鹅细小病毒强毒株XKY10株的分离、鉴定及其生物学特性分析

毛文智, 邵洪泽, 姚新华, 高洪伟, 孙健, 许志林   

  1. 吉林省畜牧兽医科学研究院家禽研究所,吉林长春 130062
  • 收稿日期:2011-09-10 修回日期:1900-01-01 出版日期:2012-03-20 发布日期:2012-03-20
  • 通讯作者: 许志林

Isolation, Identification and Biological Characteristics of GPV XKY10 Strain

MAO Wen-zhi, SHAO Hong-ze, YAO Xin-hua, GAO Hong-wei, SUN Jian, XU Zhi-lin   

  1. Science Poultry Research Institute,Jilin Institute of Animal Husbandry and Veterinary,Changchun 130062,China
  • Received:2011-09-10 Revised:1900-01-01 Online:2012-03-20 Published:2012-03-20

摘要: 采用电镜观察、PCR检测、血液凝集和琼脂扩散方法来鉴定从白城某养鹅场10日龄雏鹅具有典型症状的雏鹅肝脏、脾脏和肠组织中分离的一株病毒;通过测定此病毒最小致死量致死鹅胚的平均时间(MDT)和鹅胚半数致死率(ELD50)来分析其毒力。鉴定结果表明,该病毒为鹅细小病毒。毒力分析结果表明,该毒株为强毒株。

关键词: 鹅细小病毒; 鹅胚半数致死量; 最小致死量致死鹅胚的平均时间

Abstract: Using electron microscopy, PCR testing, blood agglutination and agar method to identify a virus isolated from the liver, spleen and intestinal tissue having typical symptoms in the 10-day-old goslings goose from a goose farm in Baicheng, and then measured the minimum death dose of goose embryo lethal average time (MDT) and the half goose embryo lethality (ELD50) to analyze the virulence. The identification results indicate that the virus is goose parvovirus, and the virulence analysis shows that this strain is a virulent strain.

Key words: GPV; ELD50; MDT

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