鹅细小病毒感染雏鹅血液的蛋白/酶活性及同工酶变异性研究

doi:10.16431/j.cnki.1671-7236.2016.02.019

›› 2016, Vol. 43 ›› Issue (2): 413-422.doi: 10.16431/j.cnki.1671-7236.2016.02.019

鹅细小病毒感染雏鹅血液的蛋白/酶活性及同工酶变异性研究

朱新产¹, 王倩文¹, 朱峰伟², 李洪强¹

1. 青岛农业大学生命科学学院, 山东省高校植物生物技术重点实验室, 青岛 266109;
2. 青岛即墨畜牧兽医局, 即墨 266200

收稿日期:2015-09-17 出版日期:2016-02-20 发布日期:2016-03-02
通讯作者: 朱新产 E-mail:xczhu321@126.com
作者简介:朱新产(1959-),男,陕西武功人,博士,教授,研究方向:病毒感染致病机制
基金资助:
青岛市科技发展计划(12-1-4-5-(2)-jch);山东省自然科学基金(ZR2011CM008)

Study on the Isozyme Variability and Activity of Protein/Enzymes from Gosling Blood Infected with Goose Parvovirus

ZHU Xin-chan¹, WANG Qian-wen¹, ZHU Feng-wei², LI Hong-qiang¹

1. Key Laboratory of Plant Biotechnology in University of Shandong Province, College of Life Science, Qingdao Agriculture University, Qingdao 266109, China;
2. Jimo Bureau of Animal Husbandry and Veterinary, Jimo 266200, China

Received:2015-09-17 Online:2016-02-20 Published:2016-03-02

摘要/Abstract

摘要： 为了解鹅细小病毒(goose parvovirus,GPV)侵染的病理学致病机理,探究蛋白/酶的分子变异特征,本研究对GPV感染雏鹅血液中的蛋白质、保护酶、蛋白酶活性及其同工酶结构和功能等进行了生化—分子生物学分析.结果显示,GPV感染雏鹅的血液中(血浆和血细胞)蛋白酶活性和蛋白质含量、过氧化物酶(POD)、超氧化物歧化酶(SOD)及酯酶(Est)活性分别是对照组的1.46和1.63、1.51和1.49、1.50和1.14、1.36和1.73、1.30和1.53倍.GPV感染雏鹅的血浆中,SOD同工酶增加了2条谱带(134和239);Est同工酶增加2条快区主带,减少了2条慢区谱带;酶蛋白减少1条慢区谱带(304);蛋白质新增加4条主带(131、86、43、33 ku).而血细胞新出现2条POD同工酶谱带(93和203),分别增加了1条(160)中区SOD同工酶谱带、1个快区Est同工酶谱带、2个慢区酶蛋白谱(223和330)和4个蛋白主带(144、104、58、53 ku).提示GPV感染应激与寄主基因表达的蛋白质、保护酶、蛋白酶及同工酶相互网络协作会引起酶的谱型、活性等生化特征变异,直接或间接调控代谢途径与病理生化性能,增强机体对入侵GPV的防御应激性能.因此,蛋白质、保护酶、蛋白酶及同工酶是GPV感染的应激靶标,可作为雏鹅易感GPV致病机制研究的有效标记.

关键词: 雏鹅; 鹅细小病毒; 蛋白/酶; 保护酶; 同工酶

Abstract: In order to understand the pathogenesis of GPV (goose parvovirus),and explore the molecular variation of protein/enzyme,the protein,protective enzymes (Est,POD,SOD,protease) and isozyme from goslings blood infected with GPV were analyzed by biochemistry and molecular biology.The results showed that the activity of protease,content of protein,the activities of POD,SOD and Est (in plasma and blood corpuscle) from goslings infected with GPV were 1.46 and 1.63,1.51 and 1.49,1.50 and 1.14,1.36 and 1.73,1.30 and 1.53 times than that of control group,respectively.In the plasma of goslings infected with GPV,2 bands (134 and 239) of SOD isozyme appeared,2 fast-zone enzyme band appeared and 2 slow-zone enzyme band deleted in Est isozyme,1 slow-zone zymoprotein band (304) deleted and 4 new main protein bands (131,86,43 and 33 ku) appeared.In the blood corpuscle of goslings infected with GPV,2 new enzyme bands (93 and 203) of POD isozyme,1 medium-zone enzyme band (160) of SOD isozyme,1 fast-zone enzyme band of Est isozyme,2 slow-zone zymoprotein band (223 and 330) and 4 new main protein bands (144,104,58 and 53 ku) appeared.These results indicated that the interaction of GPV stress and host protein/enzymes and isozyme gene expression would result in the biochemical characteristics variation of activity and isozyme patterns of protein/enzymes,and directly or indirectly affect metabolic approach and pathological biochemical function on goslings infected with GPV,and enhance the defense and stress ability of GPV.Therefore,protein/enzymes and isozyme might be sensitive enzyme of GPV infection stress,and were effective marker of pathological mechanism on investigation of virus genotype interaction with genetic susceptibility of the host.

Key words: gosling; goose parvovirus; protein/enzymes; protective enzyme; isozyme

中图分类号:

S858.33

朱新产, 王倩文, 朱峰伟, 李洪强. 鹅细小病毒感染雏鹅血液的蛋白/酶活性及同工酶变异性研究[J]. , 2016, 43(2): 413-422.

ZHU Xin-chan, WANG Qian-wen, ZHU Feng-wei, LI Hong-qiang. Study on the Isozyme Variability and Activity of Protein/Enzymes from Gosling Blood Infected with Goose Parvovirus[J]. , 2016, 43(2): 413-422.

参考文献

[1] 马磊,董浩,蒋运博,等.鹅细小病毒研究进展[J].中国畜牧兽医,2011,38(6):165-168.
[2] Shao H,Lv Y,Ye J,et al.Isolation of a goose parvovirus from swan and its molecular characteristics[J].Acta Virol,2014,58(2):194-98.
[3] Liu H M,Wang H,Tian X J,et al.Complete genome sequence of goose parvovirus Y strain isolated from Muscovy ducks in China[J].Virus Genes,2014,48(1):199-202.
[4] Rozenblatt-Rosen O,Deo R C,Padi M,et al.Interpreting cancer genomes using systematic host network perturbations by tumour virus proteins[J].Nature,2012,487(7408):491-495.
[5] Manolio T A,Collins F S,Cox N J,et al.Finding the missing heritability of complex diseases[J].Nature,2009,461(7265):747-753.
[6] Surai P F,Blesbois E,Grasseau I,et al.Fatty acid composition,glutathione peroxidase and superoxide dismutase activity and total antioxidant activity of avian semen[J].Comp Biochem Physiol B Biochem Mol Biol,1998,120(3):527-533.
[7] 胡自立,刘海玲,黄武,等.鹅细小病毒SS/10株的分离鉴定及生物学特性研究[J].中国畜牧兽医,2014,41(9):56-59.
[8] Kuznetsov S B.Polymorphism of blood plasma esterases in geese of the Anser genus (Aves:Anseriformes)[J].Biochem Genet,1995,33(5-6):183-187.
[9] Daniel M B,Stuart J E.Protein Method[M].Genera,Switzerland:Department of Biochemistry University of Genera,1997.
[10] 周明远,刘均洪.过氧化物酶活性测定的研究[J].四川动物,2006,25(2):241-243.
[11] 江慧芳,王雅琴,刘春国.三种脂肪酶活力测定方法的比较及改进[J].化学与生物工程,2007,24(8):72-75.
[12] 张宏,谭竹钧.四种邻苯三酚自氧化法测定超氧化物歧化酶活性方法的比较[J].内蒙古大学学报,2002,33(6):677-681.
[13] Schmidt W.A high performance micro-dual-wavelength-spectrophotometer (MDWS) [J].J Biochem Biophys Methods,2004,58(1):15-24.
[14] 朱新产,张兆斌,黄云鸽,等.鹅细小病毒侵染雏鹅组织器官的氧化/脱氢酶及同工酶研究[J].中国畜牧兽医,2015,42(8):2081-2090.
[15] 赵永芳.生物化学技术原理及其应用[M].武汉:武汉大学出版社,1994.
[16] 朱新产,邵艳红,朱峰伟,等.鹅细小病毒感染雏鹅的免疫球蛋白IgG/IgM变异性研究[J].华北农学报,2015,30(1):42-53.
[17] 吴译夫,王忆,吴素琴.太湖鹅血清转铁蛋白类型的测定[J].中国畜牧杂志,1989,25(1):26-27.
[18] 张淑君.鹅血浆蛋白(酶)多态性的研究[J].山东家禽,1992,5(1):3-5
[19] Shah K,Nahakpam S.Heat exposure alters the expression of SOD,POD,APX and CAT isozymes and mitigates low cadmium toxicity in seedlings of sensitive and tolerant rice cultivars[J].Plant Physiol Biochem,2012,57:106-113.
[20] Surolia I,Pirnie S P,Chellappa V,et al.Functionally defective germline variants of sialic acid acetylesterase in autoimmunity[J].Nature,2010,466(7303):243-247.
[21] Patnaik B B,Biswas T D,Nayak S K,et al.Isozymic variations in specific and nonspecific esterase and its thermostability in silkworm,Bombyx mori L[J].J Environ Biol,2012,33(5):837-842.
[22] Niyomploy P,Srisomsap C,Chokchaichamnankit D,et al.Superoxide dismutase isozyme detection using two-dimensional gel electrophoresis zymograms[J].J Pharm Biomed Anal,2014,90:72-77.
[23] 段修军,王丽华,赵旭庭,等.隆太杂交仔鹅血清酶活性与同工酶型、体重关系[J].Journal of Animal Science and Veterinary Medicine,2003,22(6):4-6.
[24] 常志伟,尹华贵,谢和芳.我国鹅的繁殖育种现状及发展前景[J].安徽农业科学,2007,35(30):2922-2923.
[25] Chen S,Zhao Q,Qi Y,et al.Immunobiological activity and antiviral regulation efforts of Chinese goose (Anser cygnoides) CD8α during NGVEV and GPV infection[J].Poult Sci,2015,94(1):17-24.

鹅细小病毒感染雏鹅血液的蛋白/酶活性及同工酶变异性研究

Study on the Isozyme Variability and Activity of Protein/Enzymes from Gosling Blood Infected with Goose Parvovirus

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