复方参芩对人工感染心肌炎型细小病毒病犬的疗效试验

›› 2012, Vol. 39 ›› Issue (2): 178-182.

复方参芩对人工感染心肌炎型细小病毒病犬的疗效试验

杜林林¹, 刘娟^1,2, 刘聪¹, 李鑫¹, 吕雪¹

1. 西南大学荣昌校区中药研究开发实验室,重庆 402460;2. 重庆市药效评价工程技术研究中心,重庆 402460

收稿日期:2011-06-29 修回日期:1900-01-01 出版日期:2012-02-20 发布日期:2012-02-20
通讯作者: 刘娟

Therapeutic Experiment of Compound Shenqin on Sickness Dog of Myocarditis Parvovirus Caused by Artifical Infection

DU Lin-lin¹, LIU Juan^1,2, LIU Cong¹, LI Xin¹, LV Xue¹

1. Traditional Chinese Medicine Research and Development Laboratory, Rongchang Campus,Southwest University, Chongqing 402460,China;2. Efficacy Criterions of Chongqing Engineering Research Center,Chongqing 402460,China

Received:2011-06-29 Revised:1900-01-01 Online:2012-02-20 Published:2012-02-20

摘要/Abstract

摘要： 本试验为探讨复方参芩对人工感染心肌炎型细小病毒病犬的疗效,将犬分为空白对照组、模型组、阳性药物对照组、复方参芩组,用犬细小病毒接种建立动物模型,用复方参芩预防犬细小病毒病,应用生物信号采集系统的心电图技术测定试验动物的心率、心电图,取心脏石蜡包埋、切片、镜检。试验结果显示,攻毒后3 d,模型组犬心率和心电图异常率增加,心肌损害严重;复方参芩组犬心率保持正常,心电图异常率显著低于模型组犬,心肌组织与空白对照组相比差异不显著。试验犬感染病毒第5天,空白对照组犬未出现死亡,模型组犬死亡率为100%,复方参芩组保护率为80%,阳性药物对照组保护率为70%。表明复方参芩能有效预防心肌炎型犬细小病毒病。

关键词: 复方参芩; 犬细小病毒; 组织病理学; 心率; 心电图

Abstract: To study therapeutic experiment of compound Shenqin on sickness dog of myocarditis parvovirus caused by artifical infection. The dogs were divided into control group,model group, positive control group,compound Shenqin group. Animal model was established with canine parvovirus. And canine parvovirus disease was prevented with compound Shenqin,experimental animals the heart rate and electrocardiogram was determinated by the biological signal acquisition system of ECG technology. Then completion of animal experiments,the animals were killed and the heart was paraffin-embedded,tissue section,microscopic examination. The results showed that after infection the third day,the heart rate and disorder rate of electrocardiogram of dog in model group was increased,serious damage to myocardial;the heart rate of dog in the compound Shenqin group retains normally,the disorder rate of electrocardiogram were significantly lower than dog in the model group,cardiac tissue compared with the control group no significantly difference. After experimental animals infected the fifth day,the death rate of dog in the model group is up to 100 percent. But the control group is not appear to death. The protective rate of compound Shenqin group is up to 80 percent. The protective rate of positive control group is up to 70 percent. The result showed that compound Shenpin can be effective in preventing myocarditis canine parvovirus disease in dog.

Key words: compound Shenqin; canine parvovirus; histopathology; heart rate; ECG

中图分类号:

S853.7

杜林林;刘娟;刘聪;李鑫;吕雪. 复方参芩对人工感染心肌炎型细小病毒病犬的疗效试验[J]. , 2012, 39(2): 178-182.

DU Lin-lin;LIU Juan;LIU Cong;LI Xin;LV Xue. Therapeutic Experiment of Compound Shenqin on Sickness Dog of Myocarditis Parvovirus Caused by Artifical Infection[J]. , 2012, 39(2): 178-182.

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