猪繁殖与呼吸综合征病毒诊断方法的研究进展

摘要/Abstract

摘要： 猪繁殖与呼吸综合征(PRRS)是严重危害养猪业的传染病之一,自2006年夏秋季节,中国自南向北相继暴发高致病性猪繁殖与呼吸综合征,采取快速准确的诊断方法与技术确定病原在PRRS的整个防控过程中具有举足轻重的作用。作者详细介绍了PRRSV诊断方法和技术的最新进展,以期为猪繁殖与呼吸综合征的诊断与防控提供技术支持和参考。

关键词: 猪繁殖与呼吸综合征; 诊断技术方法; 研究新进展

Abstract: Porcine reproductive and respiratory syndrome(PRRS) is one of serious swine diseases that has been devastating the swine industry. Since 2006, HP-PRRS has been outbroken from south to north in China,fast and accurate diagnostic methods and techniques to detect the pathogen play decisive roles in the entire PRRS prevention and control. This paper describe the PRRSV diagnostic methods and the latest technology in order to provide technical support and reference for PRRS diagnosis and prevention.

Key words: porcine reproductive and respiratory syndrome; diagnostic methods and techniques; research progress

中图分类号:

S852.65⁺9.6

苗立中, 沈志强. 猪繁殖与呼吸综合征病毒诊断方法的研究进展[J]. , 2012, 39(10): 206-210.

MIAO Li-zhong, SHEN Zhi-qiang. Progress of Diagnostic Methods of Porcine Reproductive and Respiratory Syndrome Virus[J]. , 2012, 39(10): 206-210.

参考文献

1 王刚,张鹤晓,甘孟侯,等. IPMA检测猪生殖和呼吸综合征病毒抗体的研究[J].中国兽医杂志,1996a,22(12):3~5.
2 王刚,张鹤晓,甘孟侯,等. Dot-ELISA检测猪生殖和呼吸综合征抗体的研究[J].中国兽医杂志,1996b,22(10):3~5.
3 王荣,孔繁德,陈琼,等. SYBR Green Ⅰ实时荧光定量PCR检测猪繁殖与呼吸综合征病毒[J].检验检疫学刊,2009,19(2):15~19.
4 付云云,单悦,郎广平,等. 猪繁殖与呼吸综合征病毒蛋白质研究进展[J].中国畜牧兽医,2009,39(4):60~63.
5 仝利剑,赵建增,张社民,等. 猪蓝耳病病毒抗体双抗原夹心ELISA方法的建立及初步应用[J].中国兽医学报,2010(10):1273~1276.
6 刘长龙,张建武,韦祖樟,等.猪繁殖与呼吸综合征病毒TaqMan荧光定量RT-PCR方法的建立与应用[J].中国预防兽医学报,2010,32(11):858~861.
7 张跃伟,李旭妮,郭盼盼,等. 荧光显色在环介导等温扩增(LAMP)检测猪繁殖与呼吸综合征病毒的应用[J].农业生物技术学报, 2010,18(3):508~513.
8 杨宗照,母安雄,方维焕. 高致病性猪繁殖与呼吸综合征病毒实时荧光定量PCR方法的建立[J].中国预防兽医学报,2009,31(4):288~291.
9 杨林,潘全会,曹巧玲,等. 猪繁殖与呼吸障碍综合征病毒基因芯片检测技术的建立与优化[J].中国动物检疫,2007,24(5):25~28.
10 罗长保,李树根,孙颖杰,等. 间接酶联免疫吸附试验检测猪生殖与呼吸系统综合征血清抗体的研究[J].中国兽医杂志,1997,23(10):6~7.
11 祝秀梅,刘学荣,牟克斌. 实时荧光定量PCR在猪繁殖与呼吸综合征病毒研究中的应用[J].生物技术通报,2010(8):40~44.
12 赵耘,张广州,秦玉明,等. 套式RT-PCR检测猪繁殖与呼吸综合征病毒的研究[J].微生物学通报,2003,30(6):77~80.
13 郭焕成,席进,李江南,等. 高致病性猪繁殖与呼吸综合征病毒Nsp2缺失变异株与经典美洲型毒株基因芯片鉴别方法的建立[J].中国生物制品学杂志,2010,23(11):1254~1259.
14 崔尚金,姜建宏,周艳君,等. 猪繁殖与呼吸综合征胶体金抗体检测技术的建立和初步应用[J].中国预防兽医学报,2005,27(3):213~217.
15 梁海霞,薛青红,高金源,等. 高致病性猪生殖与呼吸综合征病毒NASBA-ELISA检测方法的建立[J].中国兽医科学,2009,39(5):401~404.
16 谭斌,刘长明,危艳武,等. PRRSV-IPMA抗体检测试剂盒的研制及其应用[J].中国兽医科学,2006,36(11):880~884.
17 鑫婷,侯绍华,贾红,等. 猪呼吸与繁殖综合症病毒RT-LAMP检测方法的建立[J].中国农业科学, 2010,43(1):185~191.
18 Albina E,Leforban Y,Baron T,et al. An enzyme linked immunosorbent assay (ELISA) for the detection of antibodies to the porcine reproductive and respiratory syndrome (PRRS) virus[J].Ann Rech Vet, 1992,23(2):167~176.
19 Benson J E,Yaeger M J,Christopher-Hennings J,et al. A comparison of virus isolation,immunohistochemistry,fetal serology, and reverse-transcription polymerase chain reaction assay for the identification of porcine reproductive and respiratory syndrome virus transplacental infection in the fetus[J].J Vet Diagn Invest,2002,14(1):8~14.
20 Botner A,Nielsen J,Bille-Hansen V. Isolation of porcine reproductive and respiratory syndrome (PRRS) virus in a Danish swine herd and experimental infection of pregnant gilts with the virus[J].Vet Microbiol,1994,40(3~4):351~360.
21 Callahan J,Christopher-Hennings J,Nelson W M. Development of a comprehensive set of real-time RT-PCR assays for the detection of porcine respiratory and reproductive syndrome virus in semen, serum, and tissue[J].New Diagnostic Technology: Applications in Animal Health and Biologics Controls,2006,126:147~148.
22 Chen H T,Zhang J, Sun D H,et al.Reverse transcription loop-mediated isothermal amplification for the detection of highly pathogenic porcine reproductive and respiratory syndrome virus[J].J Virol Methods,2008,153(2):266~268.
23 Chen Q,Jian L,Xue-En F,et al. Rapid detection of porcine reproductive and respiratory syndrome virus by reverse transcription loop-mediated isothermal amplification[J].Intervirology,2009,52(2):86~91.
24 Cheon D S,Chae C. Polymerase chain reaction-based restriction fragment length polymorphism pattern of porcine reproductive and respiratory syndrome virus directly from lung tissues without virus isolation in Korea[J].J Vet Med Sci, 2001,63(5):567~571.
25 Chueh L L,Lee K H,Jeng C R,et al. A sensitive fluorescence in situ hybridization technique for detection of porcine reproductive and respiratory syndrome virus[J].J Virol Methods,1999,79(2):133~140.
26 Drew T W. Comparative serology of porcine reproductive and respiratory syndrome in eight European laboratories,using immunoperoxidase monolayer assay and enzyme-linked immunosorbent assay[J].Rev Sci Tech,1995,14(3):761~775.
27 Houben S,Callebaut P,Pensaert M B. Comparative study of a blocking enzyme-linked immunosorbent assay and the immunoperoxidase monolayer assay for the detection of antibodies to the porcine reproductive and respiratory syndrome virus in pigs[J].J Virol Methods,1995,51(1):125~128.
28 Li Q,Zhou Q F,Xue C Y,et al. Rapid detection of porcine reproductive and respiratory syndrome virus by reverse transcription loop-mediated isothermal amplification assay[J].J Virol Methods,2009,155(1):55~60.
29 Lurchachaiwong W,Payungporn S,Srisatidnarakul U, et al. Rapid detection and strain identification of porcine reproductive and respiratory syndrome virus (PRRSV) by real-time RT-PCR[J].Lett Appl Microbiol,2008,46(1):55~60.
30 Mallis L M,Raushel F M,Russell D H. Differentiation of isotopically labeled nucleotides using fast atom bombardment tandem mass spectrometry[J].Anal Chem,1987,59(7):980~984.
31 Mallis L M,Russell D H. Fast atom bombardment-tandem mass spectrometry studies of organo-alkali metal ions of small peptides[J].Anal Chem,1986,58(6):1076~1080.
32 Mardassi H,Wilson L,Mounir S,et al. Detection of porcine reproductive and respiratory syndrome virus and efficient differentiation between Canadian and European strains by reverse transcription and PCR amplification[J].J Clin Microbiol, 1994,32(9):2197~2203.
33 Martinez E,Riera P,Sitja M,et al. Simultaneous detection and genotyping of porcine reproductive and respiratory syndrome virus (PRRSV) by real-time RT-PCR and amplicon melting curve analysis using SYBR Green[J].Res Vet Sci,2008,85(1):184~193.
34 Nodelijk G,Wensvoort G,Kroese B,et al. Comparison of a commercial ELISA and an immunoperoxidase monolayer assay to detect antibodies directed against porcine respiratory and reproductive syndrome virus[J].Vet Microbiol,1996,49(3~4):285~295.
35 Notomi T,Okayama H,Masubuchi H,et al. Loop-mediated isothermal amplification of DNA[J].Nucleic Acids Res, 2000,28(12): E63.
36 Rovira A,Abrahante J,Murtaugh M, et al. Reverse transcription loop-mediated isothermal amplification for the detection of porcine reproductive and respiratory syndrome virus[J].J Vet Diagn Invest,2009,21(3):350~354.
37 Shimizu M,Yamada S,Murakami Y,et al. Isolation of porcine reproductive and respiratory syndrome (PRRS) virus from Heko-Heko disease of pigs[J].J Vet Med Sci,1994,56(2):389~391.
38 Suarez P,Zardoya R,Prieto C,et al. Direct detection of the porcine reproductive and respiratory syndrome (PRRS) virus by reverse polymerase chain reaction(RT-PCR)[J].Arch Virol,1994,135(1~2):89~99.
39 Valicek L,Psikal I,Smid B,et al. Isolation and identification of porcine reproductive and respiratory syndrome virus in cell cultures[J].Vet Med(Praha),1997,42(10):281~287.
40 Yahara Y,Ohkubo Y,Kariwa H,et al. Evaluation of enzyme-linked immunosorbent assay (ELISA) and immunofluorescent antibody (IFA) test for the detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibody in pigs from conventional farms[J].J Vet Med Sci,2002,64(7):583~588.
41 Yoon I J,Joo H S,Goyal S M,et al. A modified serum neutralization test for the detection of antibody to porcine reproductive and respiratory syndrome virus in swine sera[J].J Vet Diagn Invest,1994,6(3):289~292.

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