›› 2012, Vol. 39 ›› Issue (10): 169-172.

• 遗传繁育 • 上一篇    下一篇

大白猪、长白猪和杜洛克猪RBP4基因多态性及其与繁殖性能的相关分析

刘璐1, 梁永晔2, 吴正常1, 苏先敏1, 郑先瑞1, 王瑾1, 吴圣龙1,3, 包文斌1,3   

  1. 1. 扬州大学动物科学与技术学院,江苏扬州 225009;
    2. 扬州市宝应县畜牧兽医站,江苏宝应 225800;
    3. 江苏省扬州规模猪场高效健康养殖公共技术服务中心,江苏扬州 225009
  • 出版日期:2012-10-20 发布日期:2012-10-19
  • 通讯作者: 吴圣龙(1963-),男,江苏人,研究员,博士,研究方向:动物遗传育种与繁殖。E-mail: slwu@yzu.edu.cn E-mail:slwu@yzu.edu.cn
  • 作者简介:刘璐(1986-),女,黑龙江人,硕士生,研究方向:动物遗传育种与繁殖。
  • 基金资助:
    江苏高校优势学科建设工程资助项目、江苏省科技支撑计划(农业)(BE2009330-2, BE2010450);常州市科技攻关项目(CE20092004, CE20102006)。

Association Analysis on Polymorphism of the RBP4 Gene with Reproductive Performance in Yorkshire, Landrace and Duroc Pigs

LIU Lu1, LIANG Yong-hua2, WU Zheng-chang1, SU Xian-min1, ZHENG Xian-rui1, WANG Jin1, WU Sheng-long1,3, BAO Wen-bin1,3   

  1. 1. College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, China;
    2. Animal Husbandary and Veterinary Station of Baoying County, Baoying 225800, China;
    3. Jiangsu Yangzhou Public Technical Service Centre for Efficient and Healthy Culture Technology in Large-scale Swine Farms, Yangzhou 225009, China
  • Online:2012-10-20 Published:2012-10-19

摘要: 本试验通过PCR-RFLP技术对大白猪、长白猪和杜洛克猪的视黄醇结合蛋白4(retinol binding protein 4,RBP4)基因第4外显子Msp Ⅰ酶切位点进行多态性分析,并对各基因型母猪的第2、3胎次产仔数、产活仔数、断奶仔猪数、初生窝重、21日龄的窝重及断奶成活率进行测定,进而分析RBP4基因多态性与繁殖性能的关系。结果表明,RBP4基因在3个品种中均存在多态位点;χ2适合性检验表明大白猪在该位点偏离Hardy-Weinberg平衡状态(P<0.05),而长白猪和杜洛克猪在该位点处于Hardy-Weinberg平衡状态(P>0.05)。其中,大白猪中优势基因型为BB基因型,而长白猪和杜洛克猪中优势基因型为AA基因型。在繁殖性能上,长白猪BB基因型群体第2胎总产仔数、产活仔数、断奶仔猪数、初生窝重、21日龄断奶窝重分别高于AA基因型0.97头、1.28头、0.92头、1.74 kg和4.42 kg,高于AB基因型0.92头、1.02头、0.98头、1.43 kg和4.98 kg;第3胎次中总体上BB基因型也为有利基因型。

关键词: RBP4基因; 繁殖性能; 大白猪; 长白猪; 杜洛克猪

Abstract: The polymorphisms of MspⅠ site at exton 4 of RBP4 gene in Yorkshire, Landrace and Duroc populations were detected with PCR-RFLP method, meanwhile the total number born (TNB), number born alive (NBA), number of alive weaning litter (NW), body weight of litter (BWL), weaning weight of litter (WWL) and weaning survive rate of different genotypes for the 2nd and 3rd parity were measured, and then the relationship between reproductive performance and different genotypes was analyzed in this study. The results showed that the polymorphic sites of RBP4 genes were found in all three breeds, the results of χ2 test indicated that this site of Yorkshire didn’t fit Hardy-Weinberg equilibrium(P<0.05), while this site of Landrace and Duroc fitted Hardy-Weinberg equilibrium (P>0.05) . In addition, the dominant genotype in Yorkshire was AA genotype,however in Landrace and Duroc were BB genotype. For the reproductive performance,BB genotype of Landrace in 2nd parity produced 0.97 TNB, 1.28 NBA, 0.92 NW, 1.74 kg BWL and 4.42 kg WWL more than AA genotype, and produced 0.92 TNB, 1.02 NBA, 0.98 NW, 1.43 kg BWL and 4.98 kg WWL more than AA genotype. On the whole, BB genotype was also favourable genotype in 3rd parity.

Key words: RBP4 gene; reproductive performance; Yorkshire; Landrace; Duroc

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