游泳应激对小鼠肾脏、肺脏和脑组织脂质过氧化状态的影响

›› 2012, Vol. 39 ›› Issue (10): 159-162.

游泳应激对小鼠肾脏、肺脏和脑组织脂质过氧化状态的影响

李留安^1,2, 彭峰³, 杨凤²

1. 南京农业大学畜牧学博士后科研流动站,江苏南京 210095;
2. 天津农学院动物科学系,天津 300384;
3. 河南省诸美种猪育种集团有限公司,河南正阳 463612

收稿日期:2012-02-29 出版日期:2012-10-20 发布日期:2012-10-19
作者简介:李留安(1979-),男,河南人,副教授,博士,从事动物生理生化研究。
基金资助:
中国博士后科学基金项目(2011M500937);江苏省博士后科研资助计划项目(1102078C)。

Effects of Swimming Stress on Lipid Peroxidation of Kidneys, Lungs and Brain Tissues in Mice

LI Liu-an^1,2, PENG Feng³, YANG Feng²

1. Animal Husbandry Post-doctoral Station, Nanjing Agricultural University, Nanjing 210095, China;
2. Department of Animal Science, Tianjin Agricultural University, Tianjin 300384, China;
3. Co., Ltd. of Zhumei Pig Breeding Group of Henan Province, Zhengyang 463612, China

Received:2012-02-29 Online:2012-10-20 Published:2012-10-19

摘要/Abstract

摘要： 为探讨游泳应激对小鼠肾脏、肺脏和脑组织脂质过氧化水平的影响,本试验选取50只小鼠进行游泳试验,于游泳前、游泳10、20、30、50 min后分别随机选取10只小鼠眼眶采血致死,采集肾脏、肺脏和脑组织,测定样品中MDA含量、SOD和GSH-Px活性。结果表明,10或20 min游泳应激后,小鼠肾脏、肺脏和脑组织MDA含量均显著增加(P<0.05),之后均有不同程度的降低;游泳过程中小鼠肾脏SOD活性持续升高,30和50 min后均显著升高(P<0.05),游泳20 min后肺脏SOD活性显著降低(P<0.05),30 min后有所回升,50 min后又轻微下降,游泳过程中脑组织SOD活性均无显著性变化;游泳20和30 min后,肾脏GSH-Px活性显著增加(P<0.05),50 min后显著下降(P<0.05),游泳前40 min过程中肺脏GSH-Px持续下降,30 min组显著降低(P<0.05),游泳10和20 min后,脑组织GSH-Px活性显著降低(P<0.05),之后发生波动性变化。结果提示,游泳应激显著影响小鼠肾脏、肺脏和脑组织脂质过氧化水平,推测游泳应激可能引起小鼠全身脂质过氧化反应。

关键词: 游泳应激; 小鼠; 肾脏; 肺脏; 脑组织; 脂质过氧化

Abstract: In order to research the effects of swimming stress on lipid peroxidation of kidneys, lungs and brain tissues in mice. 50 Kunming mice were conducted the swimming experiment, 10 mice selected randomly were sacrificed before swimming and at 10, 20, 30, 50 min after swimming respectively, kidneys, lungs and brain tissues of mice were collected, maleic dialdehyde (MDA) contents, superoxide dismutase (SOD) activities and glutathione peroxidase (GSH-Px) activities were determined by spectrophotometry. This results showed that MDA contents of kidneys, lungs and brain tissues of mice at 10 or 20 min after swimming were increased significantly (P<0.05), and then decreased in a different extent; SOD activities of kidneys during the swimming process continued to rise, and 30 and 50 min groups significantly increased (P<0.05), SOD activities of lungs at 20 min after swimming reduced markedly (P<0.05) and increased slightly at 30 min after swimming, a little decrease was found at 50 min after swimming again, SOD activities of brain tissues were no significant difference during the whole swimming progress; GSH-Px activities of kidneys at 20 and 30 min after swimming significantly increased (P<0.05) and decreased markedly at 50 min after swimming(P<0.05), GSH-Px activities of lungs during the first 40 min swimming process continued to decrease and significantly reduced at 30 min swimming group (P<0.05), GSH-Px activities of brain tissues at 10 and 20 min after swimming significantly decreased (P<0.05) and then fluctuant changes occured. These results indicated that lipid peroxidation of kidneys, lungs and brain tissues in mice was affected by swimming stress and speculated that swimming stress may cause lipid peroxidation reaction of the whole body of mice.

Key words: swimming stress; mice; kidneys; lungs; brain tissues; lipid peroxidation

中图分类号:

S815

李留安, 彭峰, 杨凤. 游泳应激对小鼠肾脏、肺脏和脑组织脂质过氧化状态的影响[J]. , 2012, 39(10): 159-162.

LI Liu-an, PENG Feng, YANG Feng. Effects of Swimming Stress on Lipid Peroxidation of Kidneys, Lungs and Brain Tissues in Mice[J]. , 2012, 39(10): 159-162.

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