关键词: 山羊; MSTN基因; 克隆; 生物信息学

Abstract: In this article, myostatin (MSTN)gene of goat were cloned and bioformatics analysis were studied to provide theoretical reference for further research.The results showed that the length of MSTN gene was 1128 bp which encoding 375 amnio acid,and GenBank accession number was GU183368. MSTN protein sequence of goat had highly homology compared with sheep, pig, rabbit, horse, human, bovin,which was 93%,89%,88%,88%,88%,87% and 85%, respectively. The gene had highly conservation;hydrophobic analysis showed that most amino acid were hydropathicity amino acids;protein transmembrane helices analysis showed that there were one transmembrane helices each in inside to outside helices and outside to inside helices;signal peptide analysis showed that signal peptide sequence length was 70;protein localization sites prediction showed that it was a typeⅡ membrane protein and most of the protein located in golgi body, plasma membrane and endoplasmic reticulum membrane,some in endoplasmic reticulum. The structure and function domain forecasting showed that there were 3 N-myristoylation site,2 N-glycosylation site, 5 protein kinase C phosphorylation site, 5 casein kinase Ⅱ phosphorylation site,1 tyrosine kinase phosphorylation site, 1 EF-hand calcium-binding domain,1 leucine zipper pattern, and 1 TGF -beta family signature. Secondary structure predictions showed that there were 25.33% alpha helix,3.20% beta turn,50.13% random coil,21.33% extended strand.

Key words: goat; MSTN gene; cloning; bioformatics