›› 2011, Vol. 38 ›› Issue (7): 105-107.

• 生物技术 • 上一篇    下一篇

和田黑鸡mtDNA控制区D-Loop序列分析

於建国1, 陈静波1, 多来提尼牙孜·依明2, 杨飞3, 张伟2, 邓生荣3   

  1. 1. 新疆畜牧科学院畜牧所,新疆乌鲁木齐 830000;2. 民丰县畜牧兽医局,新疆民丰 848500;3. 和田地区畜牧兽医局,新疆和田 848500
  • 收稿日期:2010-11-16 修回日期:1900-01-01 出版日期:2011-07-20 发布日期:2011-07-20
  • 通讯作者: 陈静波

Analysis of Mitochondrial DNA D-Loop Sequences in Hetian Black Chicken

YU Jian-guo1, CHEN Jing-bo1, DAOLAITINIYAZI Yi-ming2, YANG Fei3, ZHANG Wei2, DENG Sheng-rong3   

  1. 1. Xinjiang Academy of Animal Sciences,Urumqi 830000,China;2. Bureau of Animal Husbandry and Veterinary in Cele County,Minfeng 848500,China;3. Bureau of Animal Husbandry and Veterinary in Hetan Station,Hetian 848500,China
  • Received:2010-11-16 Revised:1900-01-01 Online:2011-07-20 Published:2011-07-20

摘要: 试验旨在测定和田黑鸡mtDNA D-Loop序列,并分析其与其他5个品种鸡间的同源性及亲缘关系。结果发现,和田黑鸡mtDNA D-Loop序列全长为1230 bp,用DNAMAN软件进行序列比对,发现和田黑鸡与GenBank登录的5个品种鸡的同源性都在98%以上,测定样品mtDNA D-Loop核苷酸序列中富含A+T。聚类分析结果显示,和田黑鸡与丝羽乌骨鸡、红原鸡亲缘关系较近。

关键词: 和田黑鸡; 线粒体DNA; D-Loop; 同源性; 聚类分析

Abstract: This experiment designed to measure Hetian Black chicken mtDNA D-Loop sequence, with a length of 1230 bp for more than the sequence and Hetian Black chicken and GenBank received five kinds of homology was 98% or more,the sample mtDNA D-Loop sequence A/T enriched content. Cluster analysis display Hetian Black chicken were closed to the Silky chicken and Red junglefowl.

Key words: Hetian Black chicken; mitochondria DNA; D-Loop; homology; cluster analysis

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