›› 2011, Vol. 38 ›› Issue (7): 101-105.

• 生物技术 • 上一篇    下一篇

M41型IBV对输卵管中雌激素、孕激素受体及胰岛素生长因子-Ⅰ表达的影响

路洪岩, 李玉荣, 武现军   

  1. 河北农业大学动物科技学院,河北保定 071001
  • 收稿日期:2010-12-14 修回日期:1900-01-01 出版日期:2011-07-20 发布日期:2011-07-20
  • 通讯作者: 武现军

Influence of IBV-M41 on the Expression of Estrogen Receptor,Progesterone Receptor and Insulin-like Growth Factor-Ⅰ in Oviduct of Layer Chickens

LU Hong-yan, LI Yu-rong, WU Xian-jun   

  1. College of Animal Science and Technology,Hebei Agricultural University,Baoding 071001,China
  • Received:2010-12-14 Revised:1900-01-01 Online:2011-07-20 Published:2011-07-20

摘要: 本试验通过相对定量RT-PCR检测接种M41型鸡传染性支气管炎病毒(infectious bronchitis virus,IBV)对蛋鸡输卵管各部分(蛋白部、峡部、伞部和子宫)雌激素受体(ER)、孕激素受体(PR)及胰岛素生长因子-Ⅰ(IGF-Ⅰ)mRNA表达的影响,研究IBV早期感染(10 d)抑制蛋鸡输卵管发育的作用机制。结果显示,输卵管各部均有ER、PR和IGF-Ⅰ mRNA的表达,染毒组ER、PR mRNA的表达量较正常对照组显著减少(P<0.05),而IGF-Ⅰ的表达量较正常对照组显著增加(P<0.05)。可见IBV可通过降低ER和 PR mRNA的表达水平,抑制输卵管对雌激素的应答能力而影响输卵管的发育;而IGF-Ⅰ可能是以自分泌、旁分泌方式在输卵管局部起作用,是输卵管生长的调节因子。

关键词: IBV; 蛋雏鸡; 输卵管; 雌激素受体; 孕激素受体; 胰岛素生长因子-Ⅰ

Abstract: RT-PCR was used to detect the estrogen receptors(ER)/ progesterone receptors(PR) mRNA and insulin-like growth factor-Ⅰ(IGF-Ⅰ)expression of the oviduct of the magnum, isthmus,fimbria and shell gland. The results as follows: there were expression of ER /PR mRNA and IGF-Ⅰ in oviduct,the expression of ER and PR in vaccinated of IBV-M41 was significantly decreaced (P<0.05),but the expression of IGF-Ⅰ was significantly increased (P<0.05). The study revealed that IBV could affect the development of oviduct by reducing the expression of ER and PR,and so downgrade responsing capabilities of the estrogen, IGF-Ⅰ acted on the oviduct through autocrine or paracrine. It was probable regulatory factor in the growth of leiomyom.

Key words: infectious bronchitis virus (IBV); layer chickens; oviduct; estrogen receptors(ER); progesterone receptors(PR); insulin-like growth factor-Ⅰ(IGF-Ⅰ)

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