›› 2011, Vol. 38 ›› Issue (2): 96-98.

• 生物技术 • 上一篇    下一篇

民猪脂蛋白脂酶基因在冷诱导后表达变化的研究

张冬杰,刘娣,汪亮,别墅,孙洪涛,杨国伟,张海波   

  1. (黑龙江省农业科学院,黑龙江哈尔滨 150086)
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2011-02-20 发布日期:2011-02-20
  • 通讯作者: 刘娣

Study on Expression of Lipoprotein Lipase Gene in Min Pig during Cold Induced

ZHANG Dong-jie, LIU Di, WANG Liang, BIE Shu,SUN Hong-tao, YANG Guo-wei, ZHANG Hai-bo

  

  1. (Heilongjiang Academy of Agricultural Sciences, Harbin 150086,China)
  • Received:1900-01-01 Revised:1900-01-01 Online:2011-02-20 Published:2011-02-20
  • Contact: LIU Di

摘要: 脂蛋白脂酶(lipoprotein lipase,LPL)是动物分解代谢的限速酶,是影响脂肪代谢通路中的一个重要基因。本研究将LPL基因作为影响民猪抗寒特性的候选基因,对其在心脏、肝脏、胃、脾脏、肾脏、肺脏、大肠、小肠、子宫、卵巢、背肌、腿肌、脂肪共计13个组织内的表达情况和低温冷诱导后在民猪肌肉组织内的表达变化情况进行了分析。结果表明,LPL基因在所检测的13个组织中均有表达,但表达量存在差异,心脏、背肌、腿肌、脂肪组织中的表达量较高,子宫和卵巢组织的表达量较低;LPL基因在民猪被冷诱导后表达水平无显著变化。

关键词: 民猪; LPL基因; 组织表达; 冷诱导

Abstract: Lipoprotein lipase (LPL) plays a central role in normal lipid metabolism as the key enzyme involved in the hydrolysis of triglycerides present in chylomicrons and very low density lipoproteins. In this research, LPL was seen as a candidate gene for cold resistance, analysis the expression of LPL in heart, liver, stomach, spleen, kidney, lung, large intestine, small intestin, uterus, ovary, muscles of back, leg muscle, fat tissues and during cold induced. The results showed that LPL was an abroad expression gene, but the level was different. It was higher expressed in heart, muscles of back, leg muscle and fat, but lower expressed in uterus, ovary. The expression level of LPL was not different after cold induced.

Key words: Min pig; LPL gene; expression; cold induced

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