关键词: 猪伪狂犬病; gE基因缺失疫苗; 免疫试验

Abstract: In order to master the immune effectiveness of gE gene deleted vaccine of pseudorabies virus and formulate rational immunization programs, the immunity tests had been done with domestic and imported gE gene deleted attenuated vaccine of pseudorabies virus by 4 different immune procedures in 250 sows and 1000 piglets. During the trial, according to the proportion of regular collecting the blood of immune pigs, the antibodies were detected by ELISA diagnostic kits. Trials had proved that whether domestic or imported gene deleted vaccine of pseudorabies virus could produce a good immune effects which were good whether immunization with the child, or twice a year, or every 4 months. Boar antibodies passed rate of 100%, the piglets antibodies passed rate of 100% before 49-day-old, decreased gradually after 75-day-old and disappeared after 120-day-old. To make a stronger immune system of pigs and don’t give the opportunity of the wild poisonous invasion, it proposes the appropriate time for the second booster immunization after the first immunization of the piglets. Only the boars and sows were immunized in spring and autumn, but the piglet group was not immunized and the antibodies of piglets will reduce negative after 35 days, which can not resist the wild poisonous invasion, this method of immunization should not be promoted.

Key words: pseudorabies; gE gene deleted vaccine; immunity test